Diagnostic Accuracy of a Direct Panfungal Polymerase Chain Reaction Assay Performed on Stained Cytology Slides

Diagnostic Accuracy of a Direct Panfungal Polymerase Chain Reaction Assay Performed on Stained Cytology Slides

Molecular strategies are more and more being utilized to stained cytology slides for the analysis of neoplastic and infectious ailments. Such strategies for the identification of fungi from stained cytology slides haven’t but been evaluated. This research aimed to evaluate the diagnostic accuracy of direct (with out nucleic acid isolation) panfungal polymerase chain response (PCR) adopted by sequencing for identification of fungi and oomycetes on stained cytology slides from canines, cats, horses, and different species. Thirty-six circumstances had been recognized with cytologically identifiable fungi/oomycetes and concurrent identification through fungal tradition or immunoassay.

Twenty-nine controls had been recognized with no cytologically or histologically seen organisms and a concurrent adverse fungal tradition. Direct PCR concentrating on the inner transcribed spacer area adopted by sequencing was carried out on one cytology slide from every case and management, and the sensitivity and specificity of the assay had been calculated. The sensitivity of the panfungal PCR assay carried out on stained cytology slides was 67% total, 73% excluding circumstances with oomycetes, and 86% when contemplating solely slides with plentiful fungi. The specificity was 62%, which was attributed to amplification of fungal DNA from management slides with no seen fungus and adverse tradition outcomes.

Workplace-collected blood spots deposited on filter paper had been analysed with multiplexed affinity-based protein assays and located to be appropriate for proteomics evaluation. The protein extension assay (PEA) was used to characterize 92 proteins utilizing 1.2 mm punches in repeated samples collected from 20 employees. Overall, 97.8% of the samples and 91.3% of the analysed proteins handed high quality management. Both inside and between spot correlations utilizing six replicates from the identical particular person had been above 0.99, suggesting that comparable ranges are obtained from a number of punches from the identical spot and from consecutive spots.

Protein ranges from dried blood and moist serum from the identical people had been in contrast and the bulk of the analysed proteins had been discovered to be considerably correlated. These outcomes open up for simplified pattern assortment of blood in discipline circumstances for proteomic evaluation, but additionally spotlight that not all proteins might be robustly measured from dried complete blood. Direct panfungal PCR is succesful of offering genus- or species-level identification of fungi from stained cytology slides. Given the potential of panfungal PCR to amplify contaminant fungal DNA, this assay needs to be carried out on slides with seen fungi and interpreted along side morphologic evaluation by a medical pathologist.

Paper-based loop-mediated isothermal amplification and lateral stream (LAMP-LF) assay for identification of tissues of cattle origin

The current research was made with the aims of growth and standardization of cattle particular paper-based loop-mediated isothermal amplification cum lateral stream assay (LAMP-LFA), as a Point-of-care check (POCT) for identification of tissue of cattle origin. The parts of standardized LAMP response using cattle particular primer units had been lyophilized over paper buttons, recognized finest because the provider of LAMP reagents. Based on possible LAMP amplicon, a pair of probes was designed, tagged and its hybridization with the amplified product of paper LAMP response was optimized. The parts of lateral stream assay for detection of probe hybridized LAMP merchandise had been standardized. Analysis of profitable amplification was made by utilizing HNB dye, LAMP-LFA strip, and likewise by the standard ladder-like sample on gel electrophoresis.

The assay was discovered extremely particular for cattle with an analytical sensitivity of 0.1 pg of absolute DNA. Laboratory validation carried out on samples from completely different people of cattle, coded samples, binary meat admixture, and heat-processed cattle tissues substantiated the accuracy of the assay. Comparison with pre-standardized species-specific PCR assay taken as gold requirements revealed 100% conformity. The discipline utility of the developed assay was additional established by its compatibility with the business package eliminating the prolonged DNA extraction step and storage stability of LAMP reagent provider buttons for Four months below refrigeration. Thus, the developed assay succesful of the end result inside Three h in resource-limited settings can be utilized as POCT for identification of tissue of cattle origin.

 Diagnostic Accuracy of a Direct Panfungal Polymerase Chain Reaction Assay Performed on Stained Cytology Slides

High-Speed Lens-Free Holographic Sensing of Protein Molecules Using Quantitative Agglutination Assays

Accurate, cost-effective, easy-to-use, and point-of-care sensors for protein biomarker ranges are necessary for illness diagnostics. A cheap and compact readout method that has been used for a number of diagnostic functions is lens-free holographic microscopy, which offers an ultralarge discipline of view and submicron decision when it’s coupled with pixel super-resolution strategies. Despite its potential as a diagnostic method, lens-free microscopy has not beforehand been utilized to quantitative protein molecule sensing in answer, which might simplify sensing protocols and finally allow measurements of binding kinetics in physiological circumstances.

Here, we sense interferon-γ (an immune system biomarker) and NeutrAvidin molecules in answer by combining lens-free microscopy with a one-step bead-based agglutination assay, enabled by a customized high-speed light-emitting diode (LED) array and automatic picture processing routines. We name this a quantitative large-area binding (QLAB) sensor.

Red Active Caspase Staining Kit
K2052-25 25 assays
EUR 238
Red Active Caspase-9 Staining Kit
K2055-100 100 assays
EUR 572
Red Active Caspase-3 Staining Kit
K2053-25 25 assays
EUR 238
Red Active Caspase-8 Staining Kit
K2054-25 25 assays
EUR 238
CaspGLOW? Red Active Caspase Staining Kit
K190-25
EUR 240
CaspGLOW? Red Active Caspase-3 Staining Kit
K193-25
EUR 240
CaspGLOW? Red Active Caspase-8 Staining Kit
K198-25
EUR 240
CaspGLOW Red Active Caspase 9 Staining Kit
55R-1300 25 assays
EUR 296
Description: Caspase 9 Staining Kit for use activity in the research laboratory
CaspGLOW? Red Active Caspase-9 Staining Kit
K199-100
EUR 517
Fluorescein Active Caspase-9 Staining Kit
K2051-25 25 assays
EUR 238
Red Active Caspase Staining Kit
K2052-100 100 assays
EUR 572
CaspGLOW? Fluorescein Active Caspase-9 Staining Kit
K189-25
EUR 240
CaspGLOW Red Active Caspase Staining Kit
55R-1297 25 assays
EUR 296
Description: Caspase Staining Kit for use activity in the research laboratory
Red Active Caspase-3 Staining Kit
K2053-100 100 assays
EUR 572
Red Active Caspase-8 Staining Kit
K2054-100 100 assays
EUR 572
CaspGLOW? Red Active Caspase Staining Kit
K190-100
EUR 523
Fluorescein Active Caspase Staining Kit
K2047-25 25 assays
EUR 238
CaspGLOW Red Active Caspase 3 Staining Kit
55R-1298 25 assays
EUR 296
Description: Caspase 3 Staining Kit for use activity in the research laboratory
CaspGLOW Red Active Caspase 8 Staining Kit
55R-1299 25 assays
EUR 296
Description: Caspase 8 Staining Kit for use activity in the research laboratory
CaspGLOW? Red Active Caspase-3 Staining Kit
K193-100
EUR 533
CaspGLOW? Red Active Caspase-8 Staining Kit
K198-100
EUR 523
CaspGLOW Active Caspase 9 Staining Kit
55R-1296 25 assays
EUR 296
Description: Caspase 9 Staining Kit for use activity in the research laboratory
Fluorescein Active Caspase-9 Staining Kit
K2051-100 100 assays
EUR 572
CaspGLOW? Fluorescein Active Caspase Staining Kit
K180-25
EUR 234
Fluorescein Active Caspase-12 Staining Kit
K2046-25 25 assays
EUR 238
Fluorescein Active Caspase-2 Staining Kit
K2048-25 25 assays
EUR 238
Fluorescein Active Caspase-3 Staining Kit
K2049-25 25 assays
EUR 238
Fluorescein Active Caspase-8 Staining Kit
K2050-25 25 assays
EUR 238
CaspGLOW? Fluorescein Active Caspase-2 Staining Kit
K182-25
EUR 234
CaspGLOW? Fluorescein Active Caspase-3 Staining Kit
K183-25
EUR 234
CaspGLOW? Fluorescein Active Caspase-8 Staining Kit
K188-25
EUR 240
CaspGLOW? Fluorescein Active Caspase-12 Staining Kit
K172-25
EUR 234
CaspGLOW? Fluorescein Active Caspase-9 Staining Kit
K189-100
EUR 550
CaspGLOW Active Caspase Staining Kit
55R-1292 25 assays
EUR 296
Description: Caspase Staining Kit for use activity in the research laboratory
CaspGLOW™ Green Active Caspase-3/7 Staining Kit
K2030-25 25 assays
EUR 227
CaspGLOW Active Caspase 12 Staining Kit
55R-1291 25 assays
EUR 282
Description: Caspase 12 Staining Kit for use activity in the research laboratory
CaspGLOW Active Caspase 2 Staining Kit
55R-1293 25 assays
EUR 284
Description: Caspase 2 Staining Kit for use activity in the research laboratory
CaspGLOW Active Caspase 3 Staining Kit
55R-1294 25 assays
EUR 296
Description: Caspase 3 Staining Kit for use activity in the research laboratory
CaspGLOW Active Caspase 8 Staining Kit
55R-1295 25 assays
EUR 296
Description: Caspase 8 Staining Kit for use activity in the research laboratory
CaspGLOW? Fluorescein Active Caspase Staining Kit
K180-100
EUR 550
Live-or-Dye NuCFix Red Staining Kit (200 assays)
32010 1kit
EUR 272
Description: Minimum order quantity: 1 unit of 1kit
CaspGLOW? Fluorescein Active Caspase-2 Staining Kit
K182-100
EUR 523
CaspGLOW? Fluorescein Active Caspase-3 Staining Kit
K183-100
EUR 550
CaspGLOW? Fluorescein Active Caspase-8 Staining Kit
K188-100
EUR 550
CaspGLOW? Fluorescein Active Caspase-12 Staining Kit
K172-100
EUR 523
Caspase 9 Assay Kit, red
KF17302 25 Tests
EUR 304
Caspase-9 (Active) Antibody
3149-100
EUR 512
Active Caspase 9 antibody
70R-11648 100 ug
EUR 645
Description: Rabbit polyclonal Active Caspase 9 antibody
Live-or-Dye NuCFix Red Staining Kit, Trial Size (50 assays)
32010-T 1kit
EUR 116
Description: Minimum order quantity: 1 unit of 1kit
Polyclonal Caspase-9 (Active) Antibody
APR00031G 0.1mg
EUR 528
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Caspase-9 (Active) . This antibody is tested and proven to work in the following applications:
Active Caspase-1, rat recombinant
7524-25
EUR 338
Red-Color AP Staining Kit
AP100R-1 50 Assays
EUR 262
  • Category: Stem Cells
Caspase-9 Fluorometric Assay Kit
K2018-25 25 assays
EUR 238
Caspase-9 Colorimetric Assay Kit
K2019-25 25 assays
EUR 251
Caspase-9 Fluorometric Assay Kit
K118-25
EUR 207
Caspase-9 Colorimetric Assay Kit
K119-25
EUR 218
Caspase-9, human recombinant
1089-25
EUR 283
Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (25 assays)
30011-1 1KIT
EUR 204
Description: Minimum order quantity: 1 unit of 1KIT
Yeast Viability Staining Kit (Green/Red)
31063-1 1kit
EUR 472
Description: Minimum order quantity: 1 unit of 1kit
StemTAG Alkaline Phosphatase Staining Kit (Red)
CBA-300 100 assays
EUR 461
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.
Lipid (Oil Red O) staining Kit
K580-24
EUR 452
Caspase-9, human recombinant protein
E1009-25 25 units
EUR 308
Description: Caspase-9 belongs to the caspase-family of cysteine proteases. Caspase-9 also exists in cells as an inactive proenzyme like other caspases. Procaspase-9 is processed at aspartate residues to form active caspase-9 during the initiation of apoptosis.
CASPASE3 DEVDR110 ASSAY (25 ASSAYS)
30008-1 1KIT
EUR 204
Description: Minimum order quantity: 1 unit of 1KIT
Sirius Red/Fast Green Collagen Staining Kit
9046 30-50 samples
EUR 137
Description: Sirius Red/Fast Green Collagen Staining Kit
Caspase-3, active
RA15046 50 ug
EUR 383
Caspase Fluorometric Substrate Set II
K133-9-25
EUR 669
Caspase Colorimetric Substrate Set II
K134-9-25
EUR 659
Cell Navigator™ Lysosome Staining Kit *Red Fluorescence*
22658 500 Tests
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Cell Navigator™ Mitochondrion Staining Kit *Red Fluorescence*
22668 500 Assays
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Caspase Fluorometric Substrate Set II Plus
K137-9-25
EUR 833
Caspase Colorimetric Substrate Set II Plus
K138-9-25
EUR 833
Active Interleukin 25 (IL25)
4-APB694Hu01
  • EUR 637.60
  • EUR 274.00
  • EUR 2116.00
  • EUR 772.00
  • EUR 1444.00
  • EUR 490.00
  • EUR 5140.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: Q9H293
  • Buffer composition: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 18.5kDa
  • Isoelectric Point: 8.6
Description: Recombinant Human Interleukin 25 expressed in: E.coli
Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Red Fluorescence*
22817 100 Tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Caspase-9
GT15045 100 ul
EUR 500
Live-or-Dye UV/448 Fixable Viability Staining Kit (200 assays)
32002 1kit
EUR 283
Description: Minimum order quantity: 1 unit of 1kit
Live-or-Dye 405/452 Fixable Viability Staining Kit (200 assays)
32003 1kit
EUR 283
Description: Minimum order quantity: 1 unit of 1kit
Live-or-Dye 488/515 Fixable Viability Staining Kit (200 assays)
32004 1kit
EUR 283
Description: Minimum order quantity: 1 unit of 1kit
Live-or-Dye 568/583 Fixable Viability Staining Kit (200 assays)
32005 1kit
EUR 283
Description: Minimum order quantity: 1 unit of 1kit
Live-or-Dye 594/614 Fixable Viability Staining Kit (200 assays)
32006 1kit
EUR 283
Description: Minimum order quantity: 1 unit of 1kit
Live-or-Dye 640/662 Fixable Viability Staining Kit (200 assays)
32007 1kit
EUR 283
Description: Minimum order quantity: 1 unit of 1kit
Live-or-Dye 750/777 Fixable Viability Staining Kit (200 assays)
32008 1kit
EUR 283
Description: Minimum order quantity: 1 unit of 1kit
Live-or-Dye 405/545 Fixable Viability Staining Kit (200 assays)
32009 1kit
EUR 283
Description: Minimum order quantity: 1 unit of 1kit
Caspase-3 (Active) Antibody
3015-100
EUR 523
Caspase-3 (Active) Antibody
3015-30T
EUR 224
Caspase-6 (Active) Antibody
3156-100
EUR 446
Active Caspase 6 antibody
70R-11655 100 ug
EUR 558
Description: Rabbit polyclonal Active Caspase 6 antibody
Active Caspase 8 antibody
70R-11724 100 ug
EUR 436
Description: Rabbit polyclonal Active Caspase 8 antibody
Caspase-8 (Active) Antibody
3259-100
EUR 338
Caspase-8 (Active) Antibody
3259-30T
EUR 146
Active Caspase 8 antibody
10R-8030 100 ug
EUR 558
Description: Mouse monoclonal Active Caspase 8 antibody
Active Caspase-3 Antibody
48667-100ul 100ul
EUR 333
Active Caspase-3 Antibody
48667-50ul 50ul
EUR 239
Active Caspase 1 (CASP1)
4-APB592Ra01
  • EUR 924.32
  • EUR 350.00
  • EUR 3191.20
  • EUR 1130.40
  • EUR 2160.80
  • EUR 682.00
  • EUR 7828.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P43527
  • Buffer composition: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 21.2kDa
  • Isoelectric Point: 7.7
Description: Recombinant Rat Caspase 1 expressed in: E.coli
Caspase 3 active Antibody
abx200557-100ug 100 ug
EUR 328
  • Shipped within 3-5 working days.
Active Caspase-3 Antibody
20-abx125287
  • EUR 592.00
  • EUR 857.00
  • EUR 411.00
  • 100 ul
  • 200 ul
  • 50 ul
  • Shipped within 5-10 working days.
Anti Caspase-3 active
CAS3PU 0,1 mg
EUR 215.1
Cell Navigator™ Lysosome Staining Kit *Deep Red Fluorescence*
22659 500 Tests
EUR 176
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200
Cell Navigator™ Mitochondrion Staining Kit *Deep Red Fluorescence*
22669 500 Assays
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

The high-speed gentle supply offers, for the primary time, pixel super-resolved imaging of >104 2 μm beads in answer present process Brownian movement, with out vital movement blur. The automated picture processing routines allow the counting of particular person beads and clusters, offering a quantitative sensor readout that relies upon on each bead and analyte concentrations. Fits to the chemical binding idea are offered. For NeutrAvidin, we discover a restrict of detection (LOD) of <27 ng/mL (450 pM) and a dynamic vary of 2-Four orders of magnitude. For mouse interferon-γ, the LOD is <Three ng/mL (200 pM) and the dynamic vary is at the very least Four orders of magnitude. The QLAB sensor holds promise for point-of-care functions in low-resource communities and the place protocol simplicity is necessary.

Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells

Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells

The means of autophagy is a necessary mobile mechanism, required to take care of basic cell well being by the elimination of dysfunctional organelles, such because the ER, peroxisomes and mitochondria, in addition to protein aggregates, and micro organism. Autophagy is an especially dynamic course of, and instruments are continually being developed to check the varied steps of this course of. This protocol particulars a technique to check the top steps of autophagy-lysosomal fusion and the formation of the autolysosome. Many strategies have been used to check the varied steps of the autophagy course of.

Here we describe the RedGreen-assay (RG-assay), an immunofluorescence-based method used to visualise the concentrating on of substrates to the autolysosome in reside cells. This method takes benefit of the low lysosomal pH and over-expression of a tandem GFP-mCherry tagged protein focused to an organelle of curiosity. While within the impartial cytosol or autophagosome, each GFP and RFP will fluoresce. However, inside the autolysosome, the GFP sign is quenched as a result of low pH surroundings and the RFP emission sign will predominate. This method is quickly quantifiable and amenable to excessive throughput experiments. Additionally, by tagging the GFP-RFP tandem fluorescent protein with organelle particular concentrating on sequences, it may be used to measure a variety of substrates of autophagy.

Quantifying the ratio of alternatively spliced mRNA variants of genes with identified various splicing variants is extremely related for many purposes. Herein, we describe the validation of a quantitative PCR design for the simplified quantification of identified mRNA splice variants. The assay makes use of a single-common primer pair, twin probe design for the willpower of splicing variants in a single nicely configuration. We used murine XBP-1 splicing variants, XBP-1S and XBP-1U, to validate and show the efficiency traits of this method. Using artificial XBP-1S and XBP-1U cDNA in addition to cDNA synthesized from mouse beta-cell line MIN6, we established the efficiency parameters and dynamic vary of the assay. Reliable quantification of each variants at various focus gradients was proven.

Platelet Isolation and Activation Assays

Platelets regulate hemostasis and are the important thing determinants of pathogenic thrombosis following atherosclerotic plaque rupture. Platelets flow into in an inactive state, however develop into activated in response to wreck to the endothelium, which exposes thrombogenic materials resembling collagen to the blood circulate. Activation leads to plenty of responses, together with secretion of soluble bioactive molecules through the discharge of alpha and dense granules, activation of membrane adhesion receptors, launch of microparticles, and externalization of phosphatidylserine.

These processes facilitate agency adhesion to websites of damage and the recruitment and activation of different platelets and leukocytes, leading to aggregation and thrombus formation. Platelet activation drives the hemostatic response, and likewise contributes to pathogenic thrombus formation. Thus, quantification of platelet-associated responses is vital to many pathophysiologically related processes. Here we describe protocols for isolating, counting, and activating platelets, and for the fast quantification of cell floor proteins utilizing circulate cytometry.

Extracellular vesicles (EVs) are produced by all domains of life together with Bacteria, Archaea and Eukarya. EVs are important for mobile physiology and comprise assorted cargo: virulence components, cell wall transforming enzymes, extracellular matrix parts and even nucleic acids and metabolites. While varied protocols for isolating EVs have been established for mammalian cells, the sphere is actively growing instruments to check EVs in different organisms.

In this protocol we describe our strategies to carry out density gradient purification of EVs in bacterial cells, permitting for separation of EV subpopulations, adopted by safety assays for EV cargo characterization. Furthermore, we devised a protocol which includes a fluorescent conjugate of fatty acids into EVs, the primary to permit reside-cell EV monitoring to look at launch of EVs, together with throughout an infection of mammalian cells by pathogenic micro organism. These protocols are highly effective instruments for EV researchers as they allow the statement of EV launch and the research of the mechanisms of their formation and launch.

 Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells

Ex vivo Drosophila Wing Imaginal Disc Culture and Furin Inhibitor Assay

Furin is an evolutionarily conserved proprotein convertase (PC) household enzyme with a broad vary of substrates which might be important for developmental, homeostatic, and illness pathways. Classical genetic approaches and in vitro biochemical or cell organic assays recognized that precursor types of most development issue household proteins are processed by Furin. To quantitatively assess the potential function of Furin in cleaving and modulating intercellular dispersion of a Drosophila signaling protein, we developed a easy assay by combining genetics, ex vivo organ tradition, pharmacological remedy, and imaging analyses.

Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (25 assays)

30011-1 1KIT
EUR 204
Description: Minimum order quantity: 1 unit of 1KIT

Caspase 9 Colorimetric Assay Kit

55R-1281 25 assays
EUR 296
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase-9 Colorimetric Assay Kit

K2019-100 100 assays
EUR 529

Caspase-9 Colorimetric Assay Kit

K2019-200 200 assays
EUR 696

Caspase-9 Colorimetric Assay Kit

K2019-400 400 assays
EUR 1156

Caspase-9 Colorimetric Assay Kit

K119-100
EUR 490

Caspase-9 Colorimetric Assay Kit

K119-200
EUR 620

Caspase-9 Colorimetric Assay Kit

K119-400
EUR 958

Caspase-5 Colorimetric Assay Kit

K2196-25 25 assays
EUR 251

Caspase-10 Colorimetric Assay Kit

K2197-25 25 assays
EUR 251

Caspase-4 Colorimetric Assay Kit

K2199-25 25 assays
EUR 266

Caspase-8 Colorimetric Assay Kit

K2013-25 25 assays
EUR 238

Caspase-6 Colorimetric Assay Kit

K2015-25 25 assays
EUR 238

Caspase-2 Colorimetric Assay Kit

K2017-25 25 assays
EUR 251

Caspase-8 Colorimetric Assay Kit

K113-25
EUR 207

Caspase-6 Colorimetric Assay Kit

K115-25
EUR 202

Caspase-2 Colorimetric Assay Kit

K117-25
EUR 213

Caspase-3 Colorimetric Assay Kit

K2008-25 25 assays
EUR 238

Caspase-1 Colorimetric Assay Kit

K2011-25 25 assays
EUR 238

Caspase-3 Colorimetric Assay Kit

K106-25
EUR 207

Caspase-5 Colorimetric Assay Kit

K123-25
EUR 213

Caspase-10 Colorimetric Assay Kit

K125-25
EUR 213

Caspase-4 Colorimetric Assay Kit

K127-25
EUR 229

Caspase-1 Colorimetric Assay Kit

K111-25
EUR 207

Caspase-9 Fluorometric Assay Kit

K2018-25 25 assays
EUR 238

Caspase-9 Fluorometric Assay Kit

K118-25
EUR 207

CASPASE3 DEVDR110 ASSAY (25 ASSAYS)

30008-1 1KIT
EUR 204
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit (100 assays)

30008-2 1KIT
EUR 383
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (100 assays)

30011-2 1KIT
EUR 383
Description: Minimum order quantity: 1 unit of 1KIT

Caspase Colorimetric Substrate Set II

K134-9-25
EUR 659

Caspase 9 Assay Kit

20-abx299021
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.

Caspase 9 Assay Kit

20-abx299035
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.

Caspase Colorimetric Substrate Set II Plus

K138-9-25
EUR 833

Caspase 3 Colorimetric Assay Kit

55R-1270 25 assays
EUR 277
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

Caspase 1 Colorimetric Assay Kit

55R-1273 25 assays
EUR 277
Description: Assay Kit for detection of Capase 1 activity in the research laboratory

Caspase 8 Colorimetric Assay Kit

55R-1275 25 assays
EUR 277
Description: Assay Kit for detection of Capase 8 activity in the research laboratory

Caspase 6 Colorimetric Assay Kit

55R-1277 25 assays
EUR 284
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 2 Colorimetric Assay Kit

55R-1279 25 assays
EUR 303
Description: Assay Kit for detection of Capase 2 activity in the research laboratory

Caspase 5 Colorimetric Assay Kit

55R-1283 25 assays
EUR 303
Description: Assay Kit for detection of Capase 5 activity in the research laboratory

Caspase 10 Colorimetric Assay Kit

55R-1285 25 assays
EUR 303
Description: Assay Kit for detection of Capase 10 activity in the research laboratory

Caspase 4 Colorimetric Assay Kit

55R-1287 25 assays
EUR 312
Description: Assay Kit for detection of Capase 4 activity in the research laboratory

Caspase-5 Colorimetric Assay Kit

K2196-100 100 assays
EUR 529

Caspase-5 Colorimetric Assay Kit

K2196-200 200 assays
EUR 725

Caspase-5 Colorimetric Assay Kit

K2196-400 400 assays
EUR 1156

Caspase-10 Colorimetric Assay Kit

K2197-100 100 assays
EUR 529

Caspase-10 Colorimetric Assay Kit

K2197-200 200 assays
EUR 738

Caspase-10 Colorimetric Assay Kit

K2197-400 400 assays
EUR 1114

Caspase-4 Colorimetric Assay Kit

K2199-100 100 assays
EUR 557

Caspase-4 Colorimetric Assay Kit

K2199-200 200 assays
EUR 738

Caspase-4 Colorimetric Assay Kit

K2199-400 400 assays
EUR 1114

Caspase-8 Colorimetric Assay Kit

K2013-100 100 assays
EUR 474

Caspase-8 Colorimetric Assay Kit

K2013-200 200 assays
EUR 696

Caspase-8 Colorimetric Assay Kit

K2013-400 400 assays
EUR 1086

Caspase-6 Colorimetric Assay Kit

K2015-100 100 assays
EUR 487

Caspase-6 Colorimetric Assay Kit

K2015-200 200 assays
EUR 696

Caspase-6 Colorimetric Assay Kit

K2015-400 400 assays
EUR 1101

Caspase-2 Colorimetric Assay Kit

K2017-100 100 assays
EUR 529

Caspase-2 Colorimetric Assay Kit

K2017-200 200 assays
EUR 725

Caspase-2 Colorimetric Assay Kit

K2017-400 400 assays
EUR 1156

Caspase-8 Colorimetric Assay Kit

K113-100
EUR 479

Caspase-8 Colorimetric Assay Kit

K113-200
EUR 620

Caspase-8 Colorimetric Assay Kit

K113-400
EUR 958

Caspase-6 Colorimetric Assay Kit

K115-100
EUR 452

Caspase-6 Colorimetric Assay Kit

K115-200
EUR 615

Caspase-6 Colorimetric Assay Kit

K115-400
EUR 958

Caspase-2 Colorimetric Assay Kit

K117-100
EUR 468

Caspase-2 Colorimetric Assay Kit

K117-200
EUR 615

Caspase-2 Colorimetric Assay Kit

K117-400
EUR 958

Caspase-3 Colorimetric Assay Kit

K2008-100 100 assays
EUR 474

Caspase-3 Colorimetric Assay Kit

K2008-200 200 assays
EUR 696

Caspase-3 Colorimetric Assay Kit

K2008-400 400 assays
EUR 1086

Caspase-1 Colorimetric Assay Kit

K2011-200 200 assays
EUR 696

Caspase-1 Colorimetric Assay Kit

K2011-400 400 assays
EUR 1086

Caspase-3 Colorimetric Assay Kit

K106-100
EUR 479

Caspase-3 Colorimetric Assay Kit

K106-200
EUR 631

Caspase-3 Colorimetric Assay Kit

K106-400
EUR 958

Caspase-5 Colorimetric Assay Kit

K123-100
EUR 468

Caspase-5 Colorimetric Assay Kit

K123-200
EUR 615

Caspase-5 Colorimetric Assay Kit

K123-400
EUR 958

Caspase-10 Colorimetric Assay Kit

K125-100
EUR 463

Caspase-10 Colorimetric Assay Kit

K125-200
EUR 615

Caspase-10 Colorimetric Assay Kit

K125-400
EUR 958

Caspase-4 Colorimetric Assay Kit

K127-100
EUR 468

Caspase-4 Colorimetric Assay Kit

K127-200
EUR 615

Caspase-4 Colorimetric Assay Kit

K127-400
EUR 958

Caspase-1 Colorimetric Assay Kit

K111-100
EUR 479

Caspase-1 Colorimetric Assay Kit

K111-200
EUR 620

Caspase-1 Colorimetric Assay Kit

K111-400
EUR 958

Caspase 9 Fluorometric Assay Kit

55R-1280 25 assays
EUR 277
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase 9 Assay Kit, green

KF17206 25 Tests
EUR 304

Caspase 9 Assay Kit, red

KF17302 25 Tests
EUR 304

Caspase-9 Fluorometric Assay Kit

K2018-100 100 assays
EUR 502

Caspase-9 Fluorometric Assay Kit

K2018-200 200 assays
EUR 669

Caspase-9 Fluorometric Assay Kit

K2018-400 400 assays
EUR 1114

Caspase-9 Fluorometric Assay Kit

K118-100
EUR 468

Caspase-9 Fluorometric Assay Kit

K118-200
EUR 615

Caspase-9 Fluorometric Assay Kit

K118-400
EUR 958

human MMP-9 activity assay kit 96-assays

QZBmmp9H 1 plate 96 assays
EUR 501
The protocol herein describes easy methods to ex vivo tradition Drosophila wing imaginal discs expressing a fluorescently tagged Drosophila Fibroblast Growth Factor (FGF, Branchless/Bnl) over an extended time period within the presence of Furin inhibitors and monitor the cleavage and intercellular dispersion of the truncated Bnl components utilizing microscopy. Although the assay described right here is for assessing the impact of Furin inhibition on Bnl cleavage within the Drosophila larval wing imaginal disc, the precept and methodology can simply be adopted for every other alerts, tissue techniques, or organisms. This technique and protocol present an assay for analyzing Furin exercise on a particular substrate by straight visualizing the spatiotemporal distribution of its truncated components in an ex vivo-cultured organ.

Gentamicin Protection Assay to Determine the Number of Intracellular Bacteria during Infection of Human TC7 Intestinal Epithelial Cells by Shigella flexneri

Gentamicin Protection Assay to Determine the Number of Intracellular Bacteria during Infection of Human TC7 Intestinal Epithelial Cells by Shigella flexneri

Shigella flexneri is an intracellular bacterial pathogen that positive aspects entry to the intestine epithelium utilizing a specialised Type III Secretion System (T3SS). Various determinants mediating this invasive an infection have been experimentally verified utilizing the classical gentamicin safety assay introduced right here. In this assay epithelial cell strains are contaminated by micro organism in vitro and the extracellular micro organism are killed by gentamicin. The internalized micro organism, that are shielded from the bactericidal motion of gentamicin, are recovered by lysing the epithelial cells and enumerated by figuring out the colonies fashioned on strong medium.

Various methods primarily based on gentle microscopy, reminiscent of immunofluorescence and micro organism expressing fluorescent proteins, are additionally used for finding out intracellular micro organism. However, these methods should not solely labor intensive and require refined tools, however principally are additionally not quantitative. Despite being a straightforward quantitative technique to research invasiveness of micro organism, the gentamicin safety assay can’t distinguish between the survival and multiplication of the internalized micro organism over longer incubation durations.

To alleviate the problems created by multiplication and dissemination of internalized micro organism, complementary assays like plaque formation assays are required. This protocol presents a straightforward and cost-effective technique to decide the invasiveness and the capability to set up an an infection of Shigella below totally different circumstances. Automatic and visible interpretation of outcome bands have been additionally in contrast for the immunochromatography-based BinaxNOW and ImmuView UATs.

Urinary antigen exams (UATs) are sometimes used to diagnose Legionnaires’ illness as they’re speedy and simple to carry out on readily obtainable urine samples with out the want for specialised abilities in contrast to standard strategies. Recently developed automated readers for UATs could present goal outcomes interpretation, particularly in instances of weak outcome bands. Using 53 outlined affected person urine samples, we evaluated the efficiency of the BinaxNOW Legionella Antigen Card (Abbott), ImmuView S. pneumoniae and Legionella (SSI Diagnostica), STANDARD F Legionella Ag FIA (SD Biosensor), and Sofia Legionella FIA (Quidel) concurrently with their respective automated readers.

SARS-CoV-2 lateral circulation assays for attainable use in nationwide covid-19 seroprevalence surveys (React 2): diagnostic accuracy research

ensitivity analyses have been carried out on sera saved from 320 earlier contributors in the React 2 programme with confirmed earlier extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) an infection. Specificity analyses have been carried out on 1000 prepandemic serum samples. 100 new contributors with confirmed earlier SARS-CoV-2 an infection attended research clinics for finger prick testing.  Laboratory sensitivity and specificity analyses have been carried out for seven LFIAs on a minimal of 200 serum samples from contributors with confirmed SARS-CoV-2 an infection and 500 prepandemic serum samples, respectively.
Three LFIAs have been discovered to have a laboratory sensitivity superior to the finger prick sensitivity of the LFIA presently utilized in React 2 seroprevalence research (84%). These LFIAs have been then additional evaluated by way of finger prick testing on contributors with confirmed earlier SARS-CoV-2 an infection: two LFIAs (Surescreen, Panbio) have been evaluated in clinics in June-July 2020 and the third LFIA (AbC-19) in September 2020. A spike protein enzyme linked immunoassay and hybrid double antigen binding assay have been used as laboratory reference requirements.
The accuracy of LFIAs in detecting immunoglobulin G (IgG) antibodies to SARS-CoV-2 in contrast with two reference requirements. The sensitivity and specificity of seven new LFIAs that have been analysed utilizing sera diverse from 69% to 100%, and from 98.6% to 100%, respectively (in contrast with the two reference requirements). Sensitivity on finger prick testing was 77% (95% confidence interval 61.4% to 88.2%) for Panbio, 86% (72.7% to 94.8%) for Surescreen, and 69% (53.8% to 81.3%) for AbC-19 in contrast with the reference requirements. Sensitivity for sera from matched medical samples carried out on AbC-19 was considerably larger with serum than finger prick at 92% (80.0% to 97.7%, P=0.01). Antibody titres diverse significantly amongst cohorts. The numbers of constructive samples recognized by finger prick in the lowest antibody titre quarter diverse amongst LFIAs.
Gentamicin Protection Assay to Determine the Number of Intracellular Bacteria during Infection of Human TC7 Intestinal Epithelial Cells by Shigella flexneri

Establishment of Novel Protein Interaction Assays between Sin3 and REST Using Surface Plasmon Resonance and Time-Resolved Fluorescence Energy Transfer

Repressor element-1 (RE-1) or neural restrictive silencer component (NRSE) certain with a zinc finger transcription repressor, RE-1 silencing transcription issue (REST, also called neural restrictive silencer issue, NRSF) has been recognized as a basic repressor component in lots of genes, together with neuronal genes. Genes regulated by REST/NRSF regulate multifaceted neuronal phenotypes, and their defects in the equipment trigger neuropathies, issues of neuron exercise), autism and so forth. In REST repressions, the N-terminal repressor area recruits Sin3B by way of its paired amphipathic helix 1 (PAH1) area, which performs an necessary function as a scaffold for histone deacetylase 1 and a pair of. This equipment has a important function in sustaining neuronal robustness.

Acetylcholinesterase Activity Assay Kit - 100 Assays

AR4001-unit unit
EUR 317

Phosphatidylglycerol Assay Kit (Fluorometric)

K488-100
EUR 566

Phosphatidylethanolamine Assay Kit (Fluorometric)

K499-100
EUR 631

Homocysteine Assay Kit (Fluorometric)

K531-100
EUR 648

Tryptophan Assay Kit (Fluorometric)

K557-100
EUR 642

Cysteine Assay Kit (Fluorometric)

K558-100
EUR 561

Phosphatidylserine Assay Kit (Fluorometric)

K565-100
EUR 620

Phenylalanine Fluorometric Assay Kit

K572-100
EUR 479

Glycine Assay Kit (Fluorometric)

K589-100
EUR 691

Ornithine Assay Kit (Fluorometric)

K939-100
EUR 588

Cardiolipin Assay Kit (Fluorometric)

K944-100
EUR 729

Inosine Fluorometric Assay Kit

K712-100
EUR 533

Methionine Assay Kit (Fluorometric)

K442-100
EUR 664

Zinc Assay Kit (Fluorometric)

K428-100
EUR 523

Adenosine Assay Kit (Fluorometric)

K327-100
EUR 805

Arginine Assay Kit (Fluorometric)

K384-100
EUR 566

Histamine Assay Kit (Fluorometric)

K386-100
EUR 533

Glutathione Fluorometric Assay Kit

K251-100
EUR 479

Glucose Fluorometric Assay Kit

K2221-100 100 assays
EUR 557

Calcium Assay Kit (Fluorometric)

K409-100
EUR 479

Phosphate Assay Kit (Fluorometric)

K420-100
EUR 349

Lactate Fluorometric Assay Kit

K2140-100 100 assays
EUR 641

Citrulline Fluorometric Assay Kit

K2002-100
EUR 620

Phosphate Assay Kit (Fluorometric)

K2076-100 100 assays
EUR 363

Glutathione Fluorometric Assay Kit

K2098-100 100 assays
EUR 502

Glucose Colorimetric/Fluorometric Assay Kit

K606-100
EUR 512

Lactate Colorimetric/Fluorometric Assay Kit

K607-100
EUR 610

Neprilysin Activity Assay Kit (Fluorometric)

K487-100
EUR 610

Adipogenesis Colorimetric/Fluorometric Assay Kit

K610-100
EUR 430

PicoProbe? Fructose Fluorometric Assay Kit

K611-100
EUR 533

Pyruvate Colorimetric/Fluorometric Assay Kit

K609-100
EUR 610

Chitotriosidase Activity Assay Kit (Fluorometric)

K512-100
EUR 620

?-Glucuronidase Activity Assay Kit (Fluorometric)

K514-100
EUR 620

Methyltransferase Activity Assay Kit (Fluorometric)

K521-100
EUR 566

Soluble Collagen Assay Kit (Fluorometric)

K532-100
EUR 620

Albumin (Albuminuria) Fluorometric Assay Kit

K550-100
EUR 523

Aspartate Colorimetric/Fluorometric Assay Kit

K552-100
EUR 490

Sphingomyelinase Activity Fluorometric Assay Kit

K574-100
EUR 457

Phosphatidylcholine Colorimetric/Fluorometric Assay Kit

K576-100
EUR 490

HDL Uptake Assay Kit (Fluorometric)

K586-100
EUR 909

Citrate Colorimetric/Fluorometric Assay Kit

K655-100
EUR 539

PicoProbeTM Phosphatidylinositol Assay Kit (Fluorometric)

K750-100
EUR 599

PicoProbe? Phosphoglucomutase Assay Kit (Fluorometric)

K770-100
EUR 751

DPP4 Activity Fluorometric Assay Kit

K779-100
EUR 544

Protease Activity Fluorometric Assay Kit

K781-100
EUR 387

Renin Activity Fluorometric Assay Kit

K800-100
EUR 523

PicoProbe? Formaldehyde Fluorometric Assay Kit

K805-100
EUR 408

Copper Detection Assay Kit (Fluorometric)

K899-100
EUR 457

Cell Transformation Assay Kit (Fluorometric)

K922-100
EUR 599

Lipoxygenase Activity Assay Kit (Fluorometric)

K978-100
EUR 653

?-Xylosidase Activity Assay Kit (Fluorometric)

K981-100
EUR 588

DL-Serine Assay Kit (Fluorometric)

K743-100
EUR 691

PicoProbe? Glucose Fluorometric Assay Kit

K688-100
EUR 550

Urokinase Activity Fluorometric Assay Kit

K728-100
EUR 544

Neuraminidase Activity Fluorometric Assay Kit

K732-100
EUR 523

Uricase Activity Assay Kit (Fluorometric)

K734-100
EUR 512

Lysophosphatidylcholine Assay Kit (Colorimetric/Fluorometric)

K735-100
EUR 610

PicoProbe? Inulin Assay Kit (Fluorometric)

K737-100
EUR 582

Deubiquitinase Activity Assay Kit (Fluorometric)

K485-100
EUR 533

HDAC6 Activity Assay Kit (Fluorometric)

K466-100
EUR 631

PicoProbe? ?-Hydroxybutyrate Fluorometric Assay Kit

K651-100
EUR 528

Alanine Colorimetric/Fluorometric Assay Kit

K652-100
EUR 479

PicoProbe? Lactulose Fluorometric Assay Kit

K662-100
EUR 648

Lactose Colorimetric/Fluorometric Assay Kit

K624-100
EUR 512

Creatinine Colorimetric/Fluorometric Assay Kit

K625-100
EUR 468

Sucrose Colorimetric/Fluorometric Assay Kit

K626-100
EUR 501

Maltose Colorimetric/Fluorometric Assay Kit

K628-100
EUR 512

Creatine Colorimetric/Fluorometric Assay Kit

K635-100
EUR 479

Sarcosine Colorimetric/Fluorometric Assay Kit

K636-100
EUR 479

PicoProbe? Lactate Fluorometric Assay Kit

K638-100
EUR 620

Glycogen Colorimetric/Fluorometric Assay Kit

K646-100
EUR 610

Starch Colorimetric/Fluorometric Assay Kit

K647-100
EUR 490

Oxaloacetate Colorimetric/Fluorometric Assay Kit

K659-100
EUR 479

Glucose Uptake Fluorometric Assay Kit

K666-100
EUR 1077

Fructose Colorimetric/Fluorometric Assay Kit

K619-100
EUR 501

Ethanol Colorimetric/Fluorometric Assay Kit

K620-100
EUR 561

Galactose Colorimetric/Fluorometric Assay Kit

K621-100
EUR 512

DPP4 Activity Fluorometric Assay Kit

K2178-100 100 assays
EUR 557

Caspase-5 Fluorometric Assay Kit

K2195-100 100 assays
EUR 502

Caspase-4 Fluorometric Assay Kit

K2198-100 100 assays
EUR 529

Alanine Colorimetric/Fluorometric Assay Kit

K2205-100 100 assays
EUR 502

Citrate Colorimetric/Fluorometric Assay Kit

K2207-100 100 assays
EUR 544

Glucose Uptake Fluorometric Assay Kit

K2212-100 100 assays
EUR 1198

GST Fluorometric Activity Assay Kit

K260-100
EUR 468

PicoProbeTM Methylglyoxal Assay Kit (Fluorometric)

K461-100
EUR 555

Glycerophosphorylcholine Assay Kit (Colorimetric/Fluorometric)

K433-100
EUR 566

PicoProbe? Threonine Assay Kit (Fluorometric)

K463-100
EUR 610

Chymotrypsin Activity Assay Kit (Fluorometric)

K352-100
EUR 490

ATP Colorimetric/Fluorometric Assay Kit

K354-100
EUR 582

ADP Colorimetric/Fluorometric Assay Kit

K355-100
EUR 582

FAD Colorimetric/Fluorometric Assay Kit

K357-100
EUR 512

Caspase-8 Fluorometric Assay Kit

K2012-100 100 assays
EUR 474

Caspase-6 Fluorometric Assay Kit

K2014-100 100 assays
EUR 474

Caspase-2 Fluorometric Assay Kit

K2016-100 100 assays
EUR 502

Caspase-9 Fluorometric Assay Kit

K2018-100 100 assays
EUR 502

Acetyl-CoA Fluorometric Assay Kit

K2028-100 100 assays
EUR 544

In this research, so as to set up protein-protein interplay assays mimicking a binding floor between Sin3B and REST, we chosen necessary amino acids from structural data of the PAH1/REST complicated after which tried to reconstitute it utilizing recombinant quick peptides derived from PAH1/REST. Initially, we validated whether or not biotinylated REST interacts with glutathione S-transferase (GST)-tagged PAH1 and whether or not one other PAH1 peptide (PAH1-FLAG) competitively binds with biotinylated REST utilizing floor plasmon resonance (SPR). We noticed a direct interplay and aggressive binding of two PAH1 peptides.

Secondly, so as to set up a high-throughput and high-dynamic-range assay, we utilized an simply carried out novel time-resolved fluorescence vitality switch (TR-FRET) assay, and intently monitored this interplay. Finally, we succeeded in establishing a novel high-quality TR-FRET assay and a novel interplay assay primarily based on SPR.