Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells

Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells

The means of autophagy is a necessary mobile mechanism, required to take care of basic cell well being by the elimination of dysfunctional organelles, such because the ER, peroxisomes and mitochondria, in addition to protein aggregates, and micro organism. Autophagy is an especially dynamic course of, and instruments are continually being developed to check the varied steps of this course of. This protocol particulars a technique to check the top steps of autophagy-lysosomal fusion and the formation of the autolysosome. Many strategies have been used to check the varied steps of the autophagy course of.

Here we describe the RedGreen-assay (RG-assay), an immunofluorescence-based method used to visualise the concentrating on of substrates to the autolysosome in reside cells. This method takes benefit of the low lysosomal pH and over-expression of a tandem GFP-mCherry tagged protein focused to an organelle of curiosity. While within the impartial cytosol or autophagosome, each GFP and RFP will fluoresce. However, inside the autolysosome, the GFP sign is quenched as a result of low pH surroundings and the RFP emission sign will predominate. This method is quickly quantifiable and amenable to excessive throughput experiments. Additionally, by tagging the GFP-RFP tandem fluorescent protein with organelle particular concentrating on sequences, it may be used to measure a variety of substrates of autophagy.

Quantifying the ratio of alternatively spliced mRNA variants of genes with identified various splicing variants is extremely related for many purposes. Herein, we describe the validation of a quantitative PCR design for the simplified quantification of identified mRNA splice variants. The assay makes use of a single-common primer pair, twin probe design for the willpower of splicing variants in a single nicely configuration. We used murine XBP-1 splicing variants, XBP-1S and XBP-1U, to validate and show the efficiency traits of this method. Using artificial XBP-1S and XBP-1U cDNA in addition to cDNA synthesized from mouse beta-cell line MIN6, we established the efficiency parameters and dynamic vary of the assay. Reliable quantification of each variants at various focus gradients was proven.

Platelet Isolation and Activation Assays

Platelets regulate hemostasis and are the important thing determinants of pathogenic thrombosis following atherosclerotic plaque rupture. Platelets flow into in an inactive state, however develop into activated in response to wreck to the endothelium, which exposes thrombogenic materials resembling collagen to the blood circulate. Activation leads to plenty of responses, together with secretion of soluble bioactive molecules through the discharge of alpha and dense granules, activation of membrane adhesion receptors, launch of microparticles, and externalization of phosphatidylserine.

These processes facilitate agency adhesion to websites of damage and the recruitment and activation of different platelets and leukocytes, leading to aggregation and thrombus formation. Platelet activation drives the hemostatic response, and likewise contributes to pathogenic thrombus formation. Thus, quantification of platelet-associated responses is vital to many pathophysiologically related processes. Here we describe protocols for isolating, counting, and activating platelets, and for the fast quantification of cell floor proteins utilizing circulate cytometry.

Extracellular vesicles (EVs) are produced by all domains of life together with Bacteria, Archaea and Eukarya. EVs are important for mobile physiology and comprise assorted cargo: virulence components, cell wall transforming enzymes, extracellular matrix parts and even nucleic acids and metabolites. While varied protocols for isolating EVs have been established for mammalian cells, the sphere is actively growing instruments to check EVs in different organisms.

In this protocol we describe our strategies to carry out density gradient purification of EVs in bacterial cells, permitting for separation of EV subpopulations, adopted by safety assays for EV cargo characterization. Furthermore, we devised a protocol which includes a fluorescent conjugate of fatty acids into EVs, the primary to permit reside-cell EV monitoring to look at launch of EVs, together with throughout an infection of mammalian cells by pathogenic micro organism. These protocols are highly effective instruments for EV researchers as they allow the statement of EV launch and the research of the mechanisms of their formation and launch.

 Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells

Ex vivo Drosophila Wing Imaginal Disc Culture and Furin Inhibitor Assay

Furin is an evolutionarily conserved proprotein convertase (PC) household enzyme with a broad vary of substrates which might be important for developmental, homeostatic, and illness pathways. Classical genetic approaches and in vitro biochemical or cell organic assays recognized that precursor types of most development issue household proteins are processed by Furin. To quantitatively assess the potential function of Furin in cleaving and modulating intercellular dispersion of a Drosophila signaling protein, we developed a easy assay by combining genetics, ex vivo organ tradition, pharmacological remedy, and imaging analyses.

Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (25 assays)

30011-1 1KIT
EUR 204
Description: Minimum order quantity: 1 unit of 1KIT

Caspase 9 Colorimetric Assay Kit

55R-1281 25 assays
EUR 296
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase-9 Colorimetric Assay Kit

K2019-100 100 assays
EUR 529

Caspase-9 Colorimetric Assay Kit

K2019-200 200 assays
EUR 696

Caspase-9 Colorimetric Assay Kit

K2019-400 400 assays
EUR 1156

Caspase-9 Colorimetric Assay Kit

K119-100
EUR 490

Caspase-9 Colorimetric Assay Kit

K119-200
EUR 620

Caspase-9 Colorimetric Assay Kit

K119-400
EUR 958

Caspase-5 Colorimetric Assay Kit

K123-25
EUR 213

Caspase-10 Colorimetric Assay Kit

K125-25
EUR 213

Caspase-4 Colorimetric Assay Kit

K127-25
EUR 229

Caspase-3 Colorimetric Assay Kit

K2008-25 25 assays
EUR 238

Caspase-1 Colorimetric Assay Kit

K2011-25 25 assays
EUR 238

Caspase-8 Colorimetric Assay Kit

K2013-25 25 assays
EUR 238

Caspase-6 Colorimetric Assay Kit

K2015-25 25 assays
EUR 238

Caspase-2 Colorimetric Assay Kit

K2017-25 25 assays
EUR 251

Caspase-5 Colorimetric Assay Kit

K2196-25 25 assays
EUR 251

Caspase-10 Colorimetric Assay Kit

K2197-25 25 assays
EUR 251

Caspase-4 Colorimetric Assay Kit

K2199-25 25 assays
EUR 266

Caspase-8 Colorimetric Assay Kit

K113-25
EUR 207

Caspase-6 Colorimetric Assay Kit

K115-25
EUR 202

Caspase-2 Colorimetric Assay Kit

K117-25
EUR 213

Caspase-3 Colorimetric Assay Kit

K106-25
EUR 207

Caspase-1 Colorimetric Assay Kit

K111-25
EUR 207

Caspase-9 Fluorometric Assay Kit

K2018-25 25 assays
EUR 238

Caspase-9 Fluorometric Assay Kit

K118-25
EUR 207

CASPASE3 DEVDR110 ASSAY (25 ASSAYS)

30008-1 1KIT
EUR 204
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit (100 assays)

30008-2 1KIT
EUR 383
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (100 assays)

30011-2 1KIT
EUR 383
Description: Minimum order quantity: 1 unit of 1KIT

Caspase Colorimetric Substrate Set II

K134-9-25
EUR 659

Caspase 9 Assay Kit

20-abx299021
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.

Caspase 9 Assay Kit

20-abx299035
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.

Caspase 3 Colorimetric Assay Kit

55R-1270 25 assays
EUR 277
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

Caspase 1 Colorimetric Assay Kit

55R-1273 25 assays
EUR 277
Description: Assay Kit for detection of Capase 1 activity in the research laboratory

Caspase 8 Colorimetric Assay Kit

55R-1275 25 assays
EUR 277
Description: Assay Kit for detection of Capase 8 activity in the research laboratory

Caspase 6 Colorimetric Assay Kit

55R-1277 25 assays
EUR 284
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 2 Colorimetric Assay Kit

55R-1279 25 assays
EUR 303
Description: Assay Kit for detection of Capase 2 activity in the research laboratory

Caspase 5 Colorimetric Assay Kit

55R-1283 25 assays
EUR 303
Description: Assay Kit for detection of Capase 5 activity in the research laboratory

Caspase 10 Colorimetric Assay Kit

55R-1285 25 assays
EUR 303
Description: Assay Kit for detection of Capase 10 activity in the research laboratory

Caspase 4 Colorimetric Assay Kit

55R-1287 25 assays
EUR 312
Description: Assay Kit for detection of Capase 4 activity in the research laboratory

Caspase-5 Colorimetric Assay Kit

K123-100
EUR 468

Caspase-5 Colorimetric Assay Kit

K123-200
EUR 615

Caspase-5 Colorimetric Assay Kit

K123-400
EUR 958

Caspase-10 Colorimetric Assay Kit

K125-100
EUR 463

Caspase-10 Colorimetric Assay Kit

K125-200
EUR 615

Caspase-10 Colorimetric Assay Kit

K125-400
EUR 958

Caspase-4 Colorimetric Assay Kit

K127-100
EUR 468

Caspase-4 Colorimetric Assay Kit

K127-200
EUR 615

Caspase-4 Colorimetric Assay Kit

K127-400
EUR 958

Caspase-3 Colorimetric Assay Kit

K2008-100 100 assays
EUR 474

Caspase-3 Colorimetric Assay Kit

K2008-200 200 assays
EUR 696

Caspase-3 Colorimetric Assay Kit

K2008-400 400 assays
EUR 1086

Caspase-1 Colorimetric Assay Kit

K2011-200 200 assays
EUR 696

Caspase-1 Colorimetric Assay Kit

K2011-400 400 assays
EUR 1086

Caspase-8 Colorimetric Assay Kit

K2013-100 100 assays
EUR 474

Caspase-8 Colorimetric Assay Kit

K2013-200 200 assays
EUR 696

Caspase-8 Colorimetric Assay Kit

K2013-400 400 assays
EUR 1086

Caspase-6 Colorimetric Assay Kit

K2015-100 100 assays
EUR 487

Caspase-6 Colorimetric Assay Kit

K2015-200 200 assays
EUR 696

Caspase-6 Colorimetric Assay Kit

K2015-400 400 assays
EUR 1101

Caspase-2 Colorimetric Assay Kit

K2017-100 100 assays
EUR 529

Caspase-2 Colorimetric Assay Kit

K2017-200 200 assays
EUR 725

Caspase-2 Colorimetric Assay Kit

K2017-400 400 assays
EUR 1156

Caspase-5 Colorimetric Assay Kit

K2196-100 100 assays
EUR 529

Caspase-5 Colorimetric Assay Kit

K2196-200 200 assays
EUR 725

Caspase-5 Colorimetric Assay Kit

K2196-400 400 assays
EUR 1156

Caspase-10 Colorimetric Assay Kit

K2197-100 100 assays
EUR 529

Caspase-10 Colorimetric Assay Kit

K2197-200 200 assays
EUR 738

Caspase-10 Colorimetric Assay Kit

K2197-400 400 assays
EUR 1114

Caspase-4 Colorimetric Assay Kit

K2199-100 100 assays
EUR 557

Caspase-4 Colorimetric Assay Kit

K2199-200 200 assays
EUR 738

Caspase-4 Colorimetric Assay Kit

K2199-400 400 assays
EUR 1114

Caspase-8 Colorimetric Assay Kit

K113-100
EUR 479

Caspase-8 Colorimetric Assay Kit

K113-200
EUR 620

Caspase-8 Colorimetric Assay Kit

K113-400
EUR 958

Caspase-6 Colorimetric Assay Kit

K115-100
EUR 452

Caspase-6 Colorimetric Assay Kit

K115-200
EUR 615

Caspase-6 Colorimetric Assay Kit

K115-400
EUR 958

Caspase-2 Colorimetric Assay Kit

K117-100
EUR 468

Caspase-2 Colorimetric Assay Kit

K117-200
EUR 615

Caspase-2 Colorimetric Assay Kit

K117-400
EUR 958

Caspase-3 Colorimetric Assay Kit

K106-100
EUR 479

Caspase-3 Colorimetric Assay Kit

K106-200
EUR 631

Caspase-3 Colorimetric Assay Kit

K106-400
EUR 958

Caspase-1 Colorimetric Assay Kit

K111-100
EUR 479

Caspase-1 Colorimetric Assay Kit

K111-200
EUR 620

Caspase-1 Colorimetric Assay Kit

K111-400
EUR 958

Caspase Colorimetric Substrate Set II Plus

K138-9-25
EUR 833

Caspase 9 Fluorometric Assay Kit

55R-1280 25 assays
EUR 277
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase-9 Fluorometric Assay Kit

K2018-100 100 assays
EUR 502

Caspase-9 Fluorometric Assay Kit

K2018-200 200 assays
EUR 669

Caspase-9 Fluorometric Assay Kit

K2018-400 400 assays
EUR 1114

Caspase-9 Fluorometric Assay Kit

K118-100
EUR 468

Caspase-9 Fluorometric Assay Kit

K118-200
EUR 615

Caspase-9 Fluorometric Assay Kit

K118-400
EUR 958

Caspase 9 Assay Kit, green

KF17206 25 Tests
EUR 304

Caspase 9 Assay Kit, red

KF17302 25 Tests
EUR 304

human MMP-9 activity assay kit 96-assays

QZBmmp9H 1 plate 96 assays
EUR 501

mouse MMP-9 activity assay kit 96-assays

QZBmmp9M 1 plate 96 assays
EUR 501

Caspase-12 Fluorometric Assay Kit

K139-25
EUR 256

Caspase-10 Fluorometric Assay Kit

K124-25
EUR 202

Caspase-4 Fluorometric Assay Kit

K126-25
EUR 213

Caspase-3 Fluorometric Assay Kit

K2007-25 25 assays
EUR 238

Caspase-1 Fluorometric Assay Kit

K2010-25 25 assays
EUR 238

Caspase-8 Fluorometric Assay Kit

K2012-25 25 assays
EUR 238

Caspase-6 Fluorometric Assay Kit

K2014-25 25 assays
EUR 238

Caspase-2 Fluorometric Assay Kit

K2016-25 25 assays
EUR 238

Caspase-12 Fluorometric Assay Kit

K2150-25 25 assays
EUR 251

Caspase-5 Fluorometric Assay Kit

K2195-25 25 assays
EUR 238

Caspase-4 Fluorometric Assay Kit

K2198-25 25 assays
EUR 251

Caspase-3 Fluorometric Assay Kit

K105-25
EUR 207

Caspase-8 Fluorometric Assay Kit

K112-25
EUR 207

Caspase-6 Fluorometric Assay Kit

K114-25
EUR 202

Caspase-2 Fluorometric Assay Kit

K116-25
EUR 202

Caspase-5 Fluorometric Assay Kit

K122-25
EUR 202

Caspase-1 Fluorometric Assay Kit

K110-25
EUR 207

hydroxyproline assay kit 96-assays

QZBhypro1 1 plate 96 assays
EUR 323

Caspase Colorimetric Substrate Set

K132-7-25
EUR 544

Caspase-9, human recombinant

1089-25
EUR 283

Fluorescein Active Caspase-9 Staining Kit

K2051-25 25 assays
EUR 238

Red Active Caspase-9 Staining Kit

K2055-25 25 assays
EUR 238

Caspase 9 Colorimetric Cell-Based ELISA Kit

EKC1089 100ul
EUR 572

Acetylcholinesterase Activity Assay Kit - 100 Assays

AR4001-unit unit
EUR 317

soluble collagen assay kit 96-assays

QZBcol1-1 1 plate 96 assays
EUR 334

hydroxyproline assay kit 2x 96-assays

QZBhypro2 2 plates 192 assays
EUR 590

total collagen assay kit 96-assays

QZBtotcol1 1 plate 96 assays
EUR 334

total protein assay kit 96-assays

QZBtotprot1 1 plate 96 assays
EUR 288

OxiSelect AOPP Assay Kit (200 assays)

STA-318 200 assays
EUR 635
Description: Advanced Oxidation Protein Products (AOPP) are uremic toxins created during oxidative stress through the reaction of plasma proteins with chlorinated oxidants such as chloramines or hypochlorous acid. AOPP levels are elevated in patients with renal complications, diabetes mellitus, and atherosclerosis, as well as HIV-positive patients. Our OxiSelect AOPP Assay Kit provides a convenient method to assess oxidative stress. The kit includes a Chloramine standard and an AOPP Human Serum Albumin conjugate for use as a positive control.

Caspase-9, human recombinant protein

E1009-25 25 units
EUR 308
Description: Caspase-9 belongs to the caspase-family of cysteine proteases. Caspase-9 also exists in cells as an inactive proenzyme like other caspases. Procaspase-9 is processed at aspartate residues to form active caspase-9 during the initiation of apoptosis.

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-25
EUR 240

CaspGLOW? Red Active Caspase-9 Staining Kit

K199-25
EUR 240

Caspase Colorimetric Substrate Set Plus

K136-7-25
EUR 707

Caspase 9 Colorimetric Cell-Based ELISA Kit (OKAG00583)

OKAG00583 96 Wells
EUR 596
Description: Description of target: ;Species reactivity: Human, Mouse, Rat;Application: ELISA;Assay info: Assay Type: Cell-Based
Subtype: None
Detection Method: Colorimetric 450 nm;Sensitivity:

MTT Cell Viability Assay Kit (1000 assays)

30006 1KIT
EUR 204
Description: Minimum order quantity: 1 unit of 1KIT

Resazurin Cell Viability Assay Kit (2500 assays)

30025-1 1KIT
EUR 181
Description: Minimum order quantity: 1 unit of 1KIT

soluble collagen assay kit 2x 96-assays

QZBcol1-2 2 plates 192 assays
EUR 614

soluble collagen assay kit 5x 96-assays

QZBcol1-5 5 plates 480 assays
EUR 1196

sensitive tissue collagen assay kit 96-assays

QZBtiscol1 1 plate 96 assays
EUR 411

total collagen assay kit 2x 96-assays

QZBtotcol2 2 plates 192 assays
EUR 614

total protein assay kit 2x 96-assays

QZBtotprot2 2 plates 192 assays
EUR 527

Glutathione Colorimetric Assay Kit

55R-1351 100 assays
EUR 706
Description: Assay Kit for detection of Glutathione activity in the research laboratory

Hexokinase Colorimetric Assay Kit

K2179-100 100 assays
EUR 627

Hemoglobin Colorimetric Assay Kit

K219-200
EUR 381

Acetoacetate Colorimetric Assay Kit

K2204-100 100 assays
EUR 795

AMP Colorimetric Assay Kit

K229-100
EUR 577

Acetate Colorimetric Assay Kit

K2209-100 100 assays
EUR 529

Heme Colorimetric Assay Kit

K2215-100 100 assays
EUR 502

Glutathione Colorimetric Assay Kit

K2106-100 100 assays
EUR 529

Glutamate Colorimetric Assay Kit

K2133-100 100 assays
EUR 544

Malate Colorimetric Assay Kit

K2139-100 100 assays
EUR 502

Calcium Colorimetric Assay Kit

K2067-250 250 assays
EUR 432

Magnesium Colorimetric Assay Kit

K2068-100 100 assays
EUR 502

Iron Colorimetric Assay Kit

K2069-100 100 assays
EUR 544

Phosphate Colorimetric Assay Kit

k2074-500 500 assays
EUR 293
The protocol herein describes easy methods to ex vivo tradition Drosophila wing imaginal discs expressing a fluorescently tagged Drosophila Fibroblast Growth Factor (FGF, Branchless/Bnl) over an extended time period within the presence of Furin inhibitors and monitor the cleavage and intercellular dispersion of the truncated Bnl components utilizing microscopy. Although the assay described right here is for assessing the impact of Furin inhibition on Bnl cleavage within the Drosophila larval wing imaginal disc, the precept and methodology can simply be adopted for every other alerts, tissue techniques, or organisms. This technique and protocol present an assay for analyzing Furin exercise on a particular substrate by straight visualizing the spatiotemporal distribution of its truncated components in an ex vivo-cultured organ.

A Highly Sensitive, Reproducible Assay for Determining 4-hydroxynonenal Protein Adducts in Biological Material

A Highly Sensitive, Reproducible Assay for Determining 4-hydroxynonenal Protein Adducts in Biological Material

Oxidative stress is related to quite a few illnesses, and markers of oxidative stress in organic materials have gotten a mainstay of each experimental and scientific/epidemiological analysis. Lipid peroxidation is a serious type of oxidative stress, however resulting from their speedy degradation and instability, lipid peroxides are notoriously tough to measure, significantly in organic specimens the place their manufacturing and removing are constantly occuring. Thus, a generally used surrogate marker of lipid peroxidation is protein adducts of 4-Hydroxynonenal (HNE), an α, β-unsaturated hydroxyalkenal (i.e., a reactive aldehyde) fashioned through degradation of oxidized polyunsaturated fatty acids (PUFAs).

HNE adducts will be measured through commercially-available immunosorbent assays, however these have their limitations resulting from extreme prices, and reproducibility amongst laboratories is difficult resulting from variability in assay sensitivity, process, and reagents. Here we current a reproducible, facile, and economically conservative protocol for quantifying HNE protein adducts. The key to this protocol is to generate HNE-adduct requirements by incubating bovine serum albumin (BSA) with HNE. These requirements are then adsorbed to immunsorbent plastic in a multi-well plate format alongside organic samples.

An enzyme-linked immunosorbent assay (ELISA) is then carried out on the multi-well plate utilizing commercially-available major and secondary antibodies, and a peroxide-based fluorescent growing reagent. This protocol is very delicate and provides benefits to business sources in that it permits for reproducible, high-throughput quantitation of HNE adducts in a lot of samples. As such, it might be helpful as a biomarker of power oxidative stress for experimental and scientific research.

Understanding the molecular mechanism governing the higher-order regulation of actin dynamics requires chemically-defined and quantitative assays. Recently, the membrane reworking giant GTPase, dynamin, has been recognized as a brand new actin cross-linking molecule. Dynamin regulates actin cytoskeleton via binding to, self-assembling round, and aligning them into actin bundles. Here we make the most of dynamin for example and current a easy protocol to investigate the actin bundling exercise in vitro. This protocol particulars the strategy for F-actin reconstitution in addition to quantitative and qualitative analyses for actin bundling exercise of dynamins. Measurement of the actin bundling exercise of different actin-binding proteins might also be utilized to this protocol with acceptable changes relying on the protein of curiosity.

Genetic identification of medicinally used Salacia species by nrDNA ITS sequences and a PCR-RFLP assay for authentication of Salacia-related well being meals

The roots and stems of a number of Salacia species have been used as conventional medicines, particularly in Ayurvedic medical system for the therapy of diabetes, rheumatism, gonorrhea, amenorrhea, pores and skin illnesses, and so forth. Due to reported proof supporting Salacia’s useful results in early-stage diabetes and different lifestyle-related illnesses, Salacia-based dietary dietary supplements and well being meals have been gaining reputation in Japan and different nations in current years. However, because of the morphological similarities between Salacia crops, significantly in the medicinally used elements (roots and stems), the authentication of the botanical identities of Salacia-derived merchandise is difficult. This research goals to develop a genetic strategy to authenticate the medicinally used Salacia species and to find out the botanical sources of the commercially obtainable Salacia-derived merchandise.
 The sequences of nuclear DNA inside transcribed spacer (ITS) and chloroplast trnK-rps16 area had been decided and in contrast between 10 plant specimens from three medicinally used Salacia species in addition to 48 samples of business crude medicine. Moreover, a PCR-restriction fragment size polymorphism (RFLP) assay was developed for speedy identification primarily based on the ITS sequences. The plant specimens from the three medicinally used Salacia species confirmed three foremost kinds of sequences in each ITS (sorts I, II, III) and trnK-rps16 (i, ii, iii) areas.
Combined the sequences of ITS and trnK-rps16 areas, S. reticulata and S. oblonga had sort I-i and kind III-iii or comparable sequences, respectively. S. chinensis had sort II-ii or II(536M)-i sequences. Forty-eight samples of business crude medicine had been recognized primarily based on ITS and trnK-rps16 DNA barcode. A handy PCR-RFLP assay utilizing Cac8I restriction enzyme was established and utilized to determine the botanical sources of well being meals merchandise bought from on-line retailers. All the twelve samples had been recognized as S. chinensis.
The nrDNA ITS sequences offered helpful info to authenticate Salacia species and to elucidate the phylogenetic relationship inside the Salacia genus. Genetic identification outcomes revealed that S. chinensis and S. reticulate are the most important sources of commercially obtainable Salacia-products. Based on the ITS sequences, a handy PCR-RFLP assay was established for the identification of the medicinally used Salacia species in addition to their derived well being meals merchandise.
 A Highly Sensitive, Reproducible Assay for Determining 4-hydroxynonenal Protein Adducts in Biological Material

The Ring-Pull Assay for Mechanical Properties of Fibrous Soft Tissues – An Analysis of the Uniaxial Approximation and a Correction for Nonlinear Thick-Walled Tissues

The ring-pull check, the place a hoop of tissue is hooked through two pins and stretched, is a well-liked biomechanical check, particularly for small arteries. Although handy and dependable, the ring check produces inhomogeneous pressure, making willpower of fabric parameters non-trivial. To decide correction elements between ring-pull-estimated and true tissue properties.  A finite-element mannequin of ring pulling was constructed for a pattern with nonlinear, anisotropic mechanical conduct typical of arteries. The pin power and pattern cross-section had been used to compute an obvious modulus at small and huge pressure, which had been in comparison with the desired properties. The ensuing corrections had been validated with experiments on porcine and ovine arteries.

Acid Phosphatase Activity Fluorometric Assay Kit

K421-500
EUR 349

Alkaline Phosphatase Activity Colorimetric Assay Kit

K2075-500 500 assays
EUR 544

Alkaline Phosphatase Activity Colorimetric Assay Kit

K412-500
EUR 539

StemTAG Alkaline Phosphatase Staining and Activity Assay Kit, Fluorometric

CBA-308 2 x 100 assays
EUR 705
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Combo Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining as well as in a 96-well plate with either colorimetric or fluorescence detection.

Alkaline Phosphatase Activity Assay Kit

55R-1402 500 assays
EUR 740
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory

Alkaline Phosphatase Activity Assay Kit

55R-1406 500 assays
EUR 438
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory

Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric)

K829-100
EUR 533

StemTAG Alkaline Phosphatase Activity Assay Kit, Colorimetric

CBA-301 100 assays
EUR 403
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Kits provide reagents for quantifying alkaline phosphatase activity in a convenient 96-well plate format, with either colorimetric or fluorescence detection.

Acetylcholinesterase Activity Assay Kit - 100 Assays

AR4001-unit unit
EUR 317

DPP4 Activity Fluorometric Assay Kit

K2178-100 100 assays
EUR 557

Calpain Activity Fluorometric Assay Kit

K240-100
EUR 588

Proteasome Activity Fluorometric Assay Kit

K245-100
EUR 479

Lysozyme Activity Assay Kit (Fluorometric)

K236-100
EUR 490

Neuraminidase Activity Fluorometric Assay Kit

K2230-100 100 assays
EUR 599

GST Fluorometric Activity Assay Kit

K2105-100 100 assays
EUR 502

Calpain Activity Fluorometric Assay Kit

K2062-100 100 assays
EUR 627

PLTP Activity Fluorometric Assay Kit

K2087-100 100 assays
EUR 669

CETP Activity Fluorometric Assay Kit

K2089-100 100 assays
EUR 725

Proteasome Activity Fluorometric Assay Kit

K2096-100 100 assays
EUR 502

GST Fluorometric Activity Assay Kit

K260-100
EUR 468

HDAC3 Activity Fluorometric Assay Kit

K343-100
EUR 479

HDAC8 Activity Fluorometric Assay Kit

K348-100
EUR 490

Thrombin Activity Fluorometric Assay Kit

K373-100
EUR 697

HDAC6 Activity Assay Kit (Fluorometric)

K466-100
EUR 631

HAT Activity Fluorometric Assay Kit

K334-100
EUR 539

Sirtuin Activity Assay Kit (Fluorometric)

K324-100
EUR 572

HDAC Activity Fluorometric Assay Kit

K330-100
EUR 490

Urokinase Activity Fluorometric Assay Kit

K728-100
EUR 544

Plasmin Activity Assay Kit (Fluorometric)

K381-100
EUR 457

Chymotrypsin Activity Assay Kit (Fluorometric)

K352-100
EUR 490

Chitotriosidase Activity Assay Kit (Fluorometric)

K512-100
EUR 620

?-Glucuronidase Activity Assay Kit (Fluorometric)

K514-100
EUR 620

Methyltransferase Activity Assay Kit (Fluorometric)

K521-100
EUR 566

Sphingomyelinase Activity Fluorometric Assay Kit

K574-100
EUR 457

Deubiquitinase Activity Assay Kit (Fluorometric)

K485-100
EUR 533

Neprilysin Activity Assay Kit (Fluorometric)

K487-100
EUR 610

DPP4 Activity Fluorometric Assay Kit

K779-100
EUR 544

Protease Activity Fluorometric Assay Kit

K781-100
EUR 387

Renin Activity Fluorometric Assay Kit

K800-100
EUR 523

Lipoxygenase Activity Assay Kit (Fluorometric)

K978-100
EUR 653

?-Xylosidase Activity Assay Kit (Fluorometric)

K981-100
EUR 588

Neuraminidase Activity Fluorometric Assay Kit

K732-100
EUR 523

Uricase Activity Assay Kit (Fluorometric)

K734-100
EUR 512

CD38 Activity Assay Kit (Fluorometric)

K2042-100 100 assays
EUR 581

Sialyltransferase Activity Assay Kit (Fluorometric)

K2048-100 100 assays
EUR 601

Acid Phosphatase Activity Colorimetric Assay Kit

K411-500
EUR 359

Alkaline Phosphatase Assay Kit

abx096002-100Assays 100 Assays
EUR 316
  • Shipped within 5-10 working days.

Alkaline Phosphatase Assay Kit

abx098406-Hitachi7060R190ml2R245ml1 Hitachi 7060; R1: 90ml×2 R2: 45ml×1
EUR 222
  • Shipped within 5-12 working days.

Alkaline Phosphatase Assay Kit

abx098406-Hitachi7170R132ml4R28ml4 Hitachi 7170; R1: 32ml×4 R2: 8ml×4
EUR 222
  • Shipped within 5-12 working days.

Alkaline Phosphatase Assay Kit

abx098406-Toshiba120R150ml4R250ml1 Toshiba 120; R1: 50ml×4 R2: 50ml×1
EUR 222
  • Shipped within 5-12 working days.

Alkaline Phosphatase Assay Kit

abx098406-Toshiba40R150ml4R250ml1 Toshiba 40; R1: 50ml×4 R2: 50ml×1
EUR 222
  • Shipped within 5-12 working days.

Alkaline Phosphatase Assay Kit

Z5030033 250 assays
EUR 660
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

StemTAG Alkaline Phosphatase Staining and Activity Assay Kit, Colorimetric

CBA-302 2 x 100 assays
EUR 641
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Combo Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining as well as in a 96-well plate with either colorimetric or fluorescence detection.

Cathepsin B Activity Fluorometric Assay Kit

K140-100
EUR 512

Cathepsin K Activity Fluorometric Assay Kit

K141-100
EUR 512

Cathepsin L Activity Fluorometric Assay Kit

K142-100
EUR 512

Cathepsin D Activity Fluorometric Assay Kit

K143-100
EUR 512

Cathepsin E Activity Assay Kit (Fluorometric)

K165-100
EUR 637

Granzyme B Activity Fluorometric Assay Kit

K168-100
EUR 533

Cathepsin S Activity Fluorometric Assay Kit

K144-100
EUR 512

Cathepsin H Activity Fluorometric Assay Kit

K145-100
EUR 479

Granzyme B Activity Fluorometric Assay Kit

K2001-100 100 assays
EUR 586

Cathepsin B Activity Fluorometric Assay Kit

K2151-100 100 assays
EUR 516

Cathepsin K Activity Fluorometric Assay Kit

K2152-100 100 assays
EUR 516

Cathepsin L Activity Fluorometric Assay Kit

K2153-100 100 assays
EUR 516

Cathepsin D Activity Fluorometric Assay Kit

K2154-100 100 assays
EUR 516

Cathepsin S Activity Fluorometric Assay Kit

K2155-100 100 assays
EUR 516

Myeloperoxidase (MPO) Fluorometric Activity Assay Kit

K2169-100 100 assays
EUR 572

Catalase Activity Colorimetric/Fluorometric Assay Kit

K2177-100 100 assays
EUR 418

Lipase Activity Fluorometric Assay Kit III

K2095-100 100 assays
EUR 586

Factor VIIIa Activity Assay Kit (Fluorometric)

K358-100
EUR 539

Beta-Secretase Activity Fluorometric Assay Kit

K360-100
EUR 620

Factor Xa Activity Fluorometric Assay Kit

K361-100
EUR 620

Factor IXa Activity Assay Kit (Fluorometric)

K364-100
EUR 490

Cytidine Deaminase Activity Assay Kit (Fluorometric)

K451-100
EUR 468

Neutrophil Elastase Activity Assay kit (Fluorometric)

K383-100
EUR 419

?-Secretase (BACE1) Activity Assay Kit (Fluorometric)

K388-100
EUR 604

Pepsin/Pepsinogen Activity Assay Kit (Fluorometric)

K446-100
EUR 468

DNAse I Activity Assay Kit (Fluorometric)

K429-100
EUR 697

HAT (H4) Activity Fluorometric Assay Kit

K336-100
EUR 501

InSitu HDAC Activity Fluorometric Assay Kit

K339-100
EUR 479

Adenosine Deaminase Activity Assay Kit (Fluorometric)

K328-100
EUR 457

Myeloperoxidase (MPO) Fluorometric Activity Assay Kit

K745-100
EUR 550

Asparaginase Activity Colorimetric/Fluorometric Assay Kit

K754-100
EUR 550

Enteropeptidase/Enterokinase Activity Fluorometric Assay Kit

K758-100
EUR 610

Lipase Activity Fluorometric Assay Kit III

K724-100
EUR 533

Phospholipase A2 Activity Assay Kit (Fluorometric)

K400-100
EUR 566

Diamine Oxidase Activity Assay Kit (Fluorometric)

K496-100
EUR 566

Oxalate Oxidase Activity Assay Kit (Fluorometric)

K509-100
EUR 479

?-L-Fucosidase Activity Assay Kit (Fluorometric)

K542-100
EUR 523

Cyclooxygenase (COX) Activity Assay Kit (Fluorometric)

K549-100
EUR 419

PLTP Activity Fluorometric Assay Kit II

K593-100
EUR 588

CETP Activity Fluorometric Assay Kit II

K595-100
EUR 642

PicoProbe? Hexokinase Activity Assay Kit (Fluorometric)

K769-100
EUR 637

Peroxidase Activity Colorimetric/Fluorometric Assay Kit

K772-100
EUR 387

Catalase Activity Colorimetric/Fluorometric Assay Kit

K773-100
EUR 419

MMP-3 Activity Fluorometric Assay Kit

K783-100
EUR 512

Rat Renin Activity Fluorometric Assay Kit

K806-100
EUR 648

Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit

K807-100
EUR 865

TEV Protease Activity Assay Kit (Fluorometric)

K842-100
EUR 501

Total Phosphodiesterase Activity Assay Kit (Fluorometric)

K927-100
EUR 615

Lysyl Oxidase Activity Assay Kit (Fluorometric)

K928-100
EUR 533

PicoProbeTM Glucokinase Activity Assay Kit (Fluorometric)

K969-100
EUR 593

Aromatase (CYP19A) Activity Assay Kit (Fluorometric)

K983-100
EUR 620

Cystathionine ? Synthase Activity Assay Kit (Fluorometric)

K998-100
EUR 620

Lipoprotein Lipase Activity Fluorometric Assay Kit

K721-100
EUR 539

Enolase Activity Colorimetric/Fluorometric Assay Kit

K691-100
EUR 794

OxiSelect Catalase Activity Assay Kit, Fluorometric

STA-339 500 assays
EUR 757
Description: Catalase is a ubiquitous enzyme that destroys hydrogen peroxides formed during oxidative stress. Our OxiSelect Catalase Activity Assay Kit measures catalase activity in less than one hour from a variety of samples including blood, cells and tissues. Each kit provides sufficient reagents to perform up to 500 assays in a 96-well microtiter plate. This includes blanks, catalase standards and unknown samples. The OxiSelect Catalase Activity Assay Kit (Fluorometric) provides a 40-fold increase in sensitivity compared to our colorimetric assay.

Alpha-Mannosidase Activity Assay Kit (Fluorometric)

K2041-100 100 assays
EUR 551

Beta-Mannosidase Activity Assay Kit (Fluorometric)

K2045-100 100 assays
EUR 551

Alkaline Sphingomyelinase Activity Assay Kit (Colorimetric)

K987-100
EUR 588

Acid Phosphatase Activity Assay Kit

55R-1405 500 assays
EUR 438
Description: Assay Kit for detection of Acid Phosphatase in the research laboratory

Acid Phosphatase Activity Assay Kit

55R-1866 500 assays
EUR 484
Description: Assay Kit for detection of Acid Phosphatase in the research laboratory

human MMP-14 activity assay kit 96-assays

QZBmmp14H 1 plate 96 assays
EUR 501

human MMP-8 activity assay kit 96-assays

QZBmmp8H 1 plate 96 assays
EUR 501

human MMP-9 activity assay kit 96-assays

QZBmmp9H 1 plate 96 assays
EUR 501

mouse MMP-9 activity assay kit 96-assays

QZBmmp9M 1 plate 96 assays
EUR 501

human MMP-7 activity assay kit 96-assays

QZMmmp7H 1 plate 96 assays
EUR 501

QuantiChrom Alkaline Phosphatase Assay Kit

DALP-250 250
EUR 362
Description: Quantitative determination of alkaline phosphatase ALP activity using stable p-nitrophenol phosphate substrate at 405nm. Kit size: 250 tests. Detection limit: 2 U/L. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

QuantiFluo Alkaline Phosphatase Assay Kit

QFAP-100 100
EUR 232
Description: Quantitative fluorimetric (360/450nm) determination of alkaline phosphatase ALP activity using stable 4-methylumbelliferyl phosphate substrate. Kit size: 100 tests. Detection limit: 0.2 U/L. Shelf life: 2 years. Shipping: RT; storage: -20°C.

Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit

K2223-100 100 assays
EUR 627

Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit

K2224-100 100 assays
EUR 418

Lysosomal ?-Glucosidase (GAA) Activity Assay Kit (Fluorometric)

K187-100
EUR 555

Choline Kinase (ChoK) Activity Assay Kit (Fluorometric)

K476-100
EUR 947

Alpha Galactosidase (?-Gal) Activity Assay Kit (Fluorometric)

K407-100
EUR 528

PicoProbeTM Glutaminase (GLS) Activity Assay Kit (Fluorometric)

K455-100
EUR 555

Soluble Epoxide Hydrolase Activity Assay Kit (Fluorometric)

K477-100
EUR 566

PicoProbe? Aldehyde Dehydrogenase Activity Fluorometric Assay Kit

K741-100
EUR 572

Ethanolamine Kinase (ETNK) Activity Assay Kit (Fluorometric)

K498-100
EUR 533

Caspase-3 Activity Assay Kit II (Fluorometric)

K533-100
EUR 512

Leucine Aminopeptidase (LAP) Activity Assay Kit (Fluorometric)

K534-100
EUR 533

PicoProbe? Malate Dehydrogenase Activity Assay Kit (Fluorometric)

K538-100
EUR 566

Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric)

K561-100
EUR 533

PicoProbe? 15-PGDH Activity Assay Kit (Fluorometric)

K562-100
EUR 533

PicoProbeTM Tyrosine Aminotransferase Activity Assay Kit (Fluorometric)

K486-100
EUR 566

Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric)

K767-100
EUR 762

Glucose Oxidase Activity Colorimetric/Fluorometric Assay Kit

K788-100
EUR 539

Beta Galactosidase (?-Gal) Activity Assay Kit (Fluorometric)

K821-100
EUR 501

HIV-1 Protease Activity Assay Kit (Fluorometric)

K825-100
EUR 566

HIV-2 Protease Activity Assay Kit (Fluorometric)

K845-100
EUR 588

RNase Activity Detection/Quantification Assay Kit (Fluorometric)

K934-100
EUR 479

Paraoxonase 1 (PON1) Activity Assay Kit (Fluorometric)

K999-100
EUR 620

Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit

K710-100
EUR 408

Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit

K709-100
EUR 610
The correction was additional utilized to experiments on mouse aortic rings to find out materials and failure properties. Calculating pressure primarily based on centerline stretch somewhat than inner-wall or outer-wall stretch afforded higher estimation of tissue properties. Additional correction elements had been developed primarily based on ring wall thickness H, centerline ring radius R c , and pin radius a. The corrected estimates for tissue properties had been in good settlement with uniaxial stretch experiments.