Diagnostic Accuracy of a Direct Panfungal Polymerase Chain Reaction Assay Performed on Stained Cytology Slides

Diagnostic Accuracy of a Direct Panfungal Polymerase Chain Reaction Assay Performed on Stained Cytology Slides

Molecular strategies are more and more being utilized to stained cytology slides for the analysis of neoplastic and infectious ailments. Such strategies for the identification of fungi from stained cytology slides haven’t but been evaluated. This research aimed to evaluate the diagnostic accuracy of direct (with out nucleic acid isolation) panfungal polymerase chain response (PCR) adopted by sequencing for identification of fungi and oomycetes on stained cytology slides from canines, cats, horses, and different species. Thirty-six circumstances had been recognized with cytologically identifiable fungi/oomycetes and concurrent identification through fungal tradition or immunoassay.

Twenty-nine controls had been recognized with no cytologically or histologically seen organisms and a concurrent adverse fungal tradition. Direct PCR concentrating on the inner transcribed spacer area adopted by sequencing was carried out on one cytology slide from every case and management, and the sensitivity and specificity of the assay had been calculated. The sensitivity of the panfungal PCR assay carried out on stained cytology slides was 67% total, 73% excluding circumstances with oomycetes, and 86% when contemplating solely slides with plentiful fungi. The specificity was 62%, which was attributed to amplification of fungal DNA from management slides with no seen fungus and adverse tradition outcomes.

Workplace-collected blood spots deposited on filter paper had been analysed with multiplexed affinity-based protein assays and located to be appropriate for proteomics evaluation. The protein extension assay (PEA) was used to characterize 92 proteins utilizing 1.2 mm punches in repeated samples collected from 20 employees. Overall, 97.8% of the samples and 91.3% of the analysed proteins handed high quality management. Both inside and between spot correlations utilizing six replicates from the identical particular person had been above 0.99, suggesting that comparable ranges are obtained from a number of punches from the identical spot and from consecutive spots.

Protein ranges from dried blood and moist serum from the identical people had been in contrast and the bulk of the analysed proteins had been discovered to be considerably correlated. These outcomes open up for simplified pattern assortment of blood in discipline circumstances for proteomic evaluation, but additionally spotlight that not all proteins might be robustly measured from dried complete blood. Direct panfungal PCR is succesful of offering genus- or species-level identification of fungi from stained cytology slides. Given the potential of panfungal PCR to amplify contaminant fungal DNA, this assay needs to be carried out on slides with seen fungi and interpreted along side morphologic evaluation by a medical pathologist.

Paper-based loop-mediated isothermal amplification and lateral stream (LAMP-LF) assay for identification of tissues of cattle origin

The current research was made with the aims of growth and standardization of cattle particular paper-based loop-mediated isothermal amplification cum lateral stream assay (LAMP-LFA), as a Point-of-care check (POCT) for identification of tissue of cattle origin. The parts of standardized LAMP response using cattle particular primer units had been lyophilized over paper buttons, recognized finest because the provider of LAMP reagents. Based on possible LAMP amplicon, a pair of probes was designed, tagged and its hybridization with the amplified product of paper LAMP response was optimized. The parts of lateral stream assay for detection of probe hybridized LAMP merchandise had been standardized. Analysis of profitable amplification was made by utilizing HNB dye, LAMP-LFA strip, and likewise by the standard ladder-like sample on gel electrophoresis.

The assay was discovered extremely particular for cattle with an analytical sensitivity of 0.1 pg of absolute DNA. Laboratory validation carried out on samples from completely different people of cattle, coded samples, binary meat admixture, and heat-processed cattle tissues substantiated the accuracy of the assay. Comparison with pre-standardized species-specific PCR assay taken as gold requirements revealed 100% conformity. The discipline utility of the developed assay was additional established by its compatibility with the business package eliminating the prolonged DNA extraction step and storage stability of LAMP reagent provider buttons for Four months below refrigeration. Thus, the developed assay succesful of the end result inside Three h in resource-limited settings can be utilized as POCT for identification of tissue of cattle origin.

 Diagnostic Accuracy of a Direct Panfungal Polymerase Chain Reaction Assay Performed on Stained Cytology Slides

High-Speed Lens-Free Holographic Sensing of Protein Molecules Using Quantitative Agglutination Assays

Accurate, cost-effective, easy-to-use, and point-of-care sensors for protein biomarker ranges are necessary for illness diagnostics. A cheap and compact readout method that has been used for a number of diagnostic functions is lens-free holographic microscopy, which offers an ultralarge discipline of view and submicron decision when it’s coupled with pixel super-resolution strategies. Despite its potential as a diagnostic method, lens-free microscopy has not beforehand been utilized to quantitative protein molecule sensing in answer, which might simplify sensing protocols and finally allow measurements of binding kinetics in physiological circumstances.

Here, we sense interferon-γ (an immune system biomarker) and NeutrAvidin molecules in answer by combining lens-free microscopy with a one-step bead-based agglutination assay, enabled by a customized high-speed light-emitting diode (LED) array and automatic picture processing routines. We name this a quantitative large-area binding (QLAB) sensor.

CaspGLOW? Red Active Caspase Staining Kit

K190-25
EUR 240

Red Active Caspase-3 Staining Kit

K2053-25 25 assays
EUR 238

Red Active Caspase-8 Staining Kit

K2054-25 25 assays
EUR 238

CaspGLOW? Red Active Caspase-3 Staining Kit

K193-25
EUR 240

CaspGLOW? Red Active Caspase-8 Staining Kit

K198-25
EUR 240

Fluorescein Active Caspase-9 Staining Kit

K2051-25 25 assays
EUR 238

CaspGLOW Red Active Caspase 9 Staining Kit

55R-1300 25 assays
EUR 296
Description: Caspase 9 Staining Kit for use activity in the research laboratory

CaspGLOW? Red Active Caspase-9 Staining Kit

K199-100
EUR 517

Red Active Caspase Staining Kit

K2052-100 100 assays
EUR 572

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-25
EUR 240

CaspGLOW Red Active Caspase Staining Kit

55R-1297 25 assays
EUR 296
Description: Caspase Staining Kit for use activity in the research laboratory

CaspGLOW? Red Active Caspase Staining Kit

K190-100
EUR 523

Red Active Caspase-3 Staining Kit

K2053-100 100 assays
EUR 572

Red Active Caspase-8 Staining Kit

K2054-100 100 assays
EUR 572

Fluorescein Active Caspase Staining Kit

K2047-25 25 assays
EUR 238

CaspGLOW Red Active Caspase 3 Staining Kit

55R-1298 25 assays
EUR 296
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase 8 Staining Kit

55R-1299 25 assays
EUR 296
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW? Red Active Caspase-3 Staining Kit

K193-100
EUR 533

CaspGLOW? Red Active Caspase-8 Staining Kit

K198-100
EUR 523

CaspGLOW Active Caspase 9 Staining Kit

55R-1296 25 assays
EUR 296
Description: Caspase 9 Staining Kit for use activity in the research laboratory

Fluorescein Active Caspase-9 Staining Kit

K2051-100 100 assays
EUR 572

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-25
EUR 234

Fluorescein Active Caspase-12 Staining Kit

K2046-25 25 assays
EUR 238

Fluorescein Active Caspase-2 Staining Kit

K2048-25 25 assays
EUR 238

Fluorescein Active Caspase-3 Staining Kit

K2049-25 25 assays
EUR 238

Fluorescein Active Caspase-8 Staining Kit

K2050-25 25 assays
EUR 238

CaspGLOW? Fluorescein Active Caspase-2 Staining Kit

K182-25
EUR 234

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit

K183-25
EUR 234

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit

K188-25
EUR 240

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit

K172-25
EUR 234

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-100
EUR 550

CaspGLOW Active Caspase Staining Kit

55R-1292 25 assays
EUR 296
Description: Caspase Staining Kit for use activity in the research laboratory

Fluorescein Active Caspase Staining Kit

K2047-100 100 assays
EUR 572

CaspGLOW™ Green Active Caspase-3/7 Staining Kit

K2030-25 25 assays
EUR 227

CaspGLOW Active Caspase 12 Staining Kit

55R-1291 25 assays
EUR 282
Description: Caspase 12 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 2 Staining Kit

55R-1293 25 assays
EUR 284
Description: Caspase 2 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 3 Staining Kit

55R-1294 25 assays
EUR 296
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 8 Staining Kit

55R-1295 25 assays
EUR 296
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-100
EUR 550

Fluorescein Active Caspase-12 Staining Kit

K2046-100 100 assays
EUR 572

Fluorescein Active Caspase-2 Staining Kit

K2048-100 100 assays
EUR 572

Fluorescein Active Caspase-3 Staining Kit

K2049-100 100 assays
EUR 572

Fluorescein Active Caspase-8 Staining Kit

K2050-100 100 assays
EUR 572

Live-or-Dye NuCFix Red Staining Kit (200 assays)

32010 1kit
EUR 272
Description: Minimum order quantity: 1 unit of 1kit

Caspase 9 Assay Kit, red

KF17302 25 Tests
EUR 304

CaspGLOW? Fluorescein Active Caspase-2 Staining Kit

K182-100
EUR 523

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit

K183-100
EUR 550

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit

K188-100
EUR 550

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit

K172-100
EUR 523

Caspase-9 (Active) Antibody

3149-100
EUR 512

Active Caspase 9 antibody

70R-11648 100 ug
EUR 645
Description: Rabbit polyclonal Active Caspase 9 antibody

Live-or-Dye NuCFix Red Staining Kit, Trial Size (50 assays)

32010-T 1kit
EUR 116
Description: Minimum order quantity: 1 unit of 1kit

Polyclonal Caspase-9 (Active) Antibody

APR00031G 0.1mg
EUR 528
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human Caspase-9 (Active) . This antibody is tested and proven to work in the following applications:

Active Caspase-1, rat recombinant

7524-25
EUR 338

Caspase-9 Fluorometric Assay Kit

K118-25
EUR 207

Caspase-9 Colorimetric Assay Kit

K119-25
EUR 218

Caspase-9 Fluorometric Assay Kit

K2018-25 25 assays
EUR 238

Caspase-9 Colorimetric Assay Kit

K2019-25 25 assays
EUR 251

Red-Color AP Staining Kit

AP100R-1 50 Assays
EUR 262

Caspase-9, human recombinant

1089-25
EUR 283

Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (25 assays)

30011-1 1KIT
EUR 204
Description: Minimum order quantity: 1 unit of 1KIT

Lipid (Oil Red O) staining Kit

K580-24
EUR 452

Yeast Viability Staining Kit (Green/Red)

31063-1 1kit
EUR 472
Description: Minimum order quantity: 1 unit of 1kit

StemTAG Alkaline Phosphatase Staining Kit (Red)

CBA-300 100 assays
EUR 461
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

Caspase-9, human recombinant protein

E1009-25 25 units
EUR 308
Description: Caspase-9 belongs to the caspase-family of cysteine proteases. Caspase-9 also exists in cells as an inactive proenzyme like other caspases. Procaspase-9 is processed at aspartate residues to form active caspase-9 during the initiation of apoptosis.

CASPASE3 DEVDR110 ASSAY (25 ASSAYS)

30008-1 1KIT
EUR 204
Description: Minimum order quantity: 1 unit of 1KIT

Sirius Red/Fast Green Collagen Staining Kit

9046 30-50 samples
EUR 137
Description: Sirius Red/Fast Green Collagen Staining Kit

Caspase Fluorometric Substrate Set II

K133-9-25
EUR 669

Caspase Colorimetric Substrate Set II

K134-9-25
EUR 659

Caspase-3, active

RA15046 50 ug
EUR 383

Caspase Fluorometric Substrate Set II Plus

K137-9-25
EUR 833

Caspase Colorimetric Substrate Set II Plus

K138-9-25
EUR 833

Caspase-9

GT15045 100 ul
EUR 500

Cell Navigator™ Lysosome Staining Kit *Red Fluorescence*

22658 500 Tests
EUR 176

Cell Navigator™ Mitochondrion Staining Kit *Red Fluorescence*

22668 500 Assays
EUR 219

Active Interleukin 25 (IL25)

4-APB694Hu01
  • EUR 637.60
  • EUR 274.00
  • EUR 2116.00
  • EUR 772.00
  • EUR 1444.00
  • EUR 490.00
  • EUR 5140.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Human Interleukin 25 expressed in: E.coli

Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Red Fluorescence*

22817 100 Tests
EUR 219

Caspase-3 (Active) Antibody

3015-100
EUR 523

Caspase-3 (Active) Antibody

3015-30T
EUR 224

Caspase-6 (Active) Antibody

3156-100
EUR 446

Active Caspase 6 antibody

70R-11655 100 ug
EUR 558
Description: Rabbit polyclonal Active Caspase 6 antibody

Active Caspase 8 antibody

70R-11724 100 ug
EUR 436
Description: Rabbit polyclonal Active Caspase 8 antibody

Caspase-8 (Active) Antibody

3259-100
EUR 338

Caspase-8 (Active) Antibody

3259-30T
EUR 146

Active Caspase 8 antibody

10R-8030 100 ug
EUR 558
Description: Mouse monoclonal Active Caspase 8 antibody

Active Caspase 1 (CASP1)

4-APB592Ra01
  • EUR 924.32
  • EUR 350.00
  • EUR 3191.20
  • EUR 1130.40
  • EUR 2160.80
  • EUR 682.00
  • EUR 7828.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Rat Caspase 1 expressed in: E.coli

Active Caspase-3 Antibody

20-abx125287
  • EUR 592.00
  • EUR 857.00
  • EUR 411.00
  • 100 ul
  • 200 ul
  • 50 ul

Caspase 3 active Antibody

abx200557-100ug 100 ug
EUR 328

Active Caspase-3 Antibody

48667-100ul 100ul
EUR 333

Active Caspase-3 Antibody

48667-50ul 50ul
EUR 239

Anti Caspase-3 active

CAS3PU 0,1 mg
EUR 215.1

Caspase 9 Assay Kit

20-abx299021
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 9 Assay Kit

20-abx299035
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Prosi Silver Staining Kit for 25 mini gel

S3200-020 1kit
EUR 361

Live-or-Dye UV/448 Fixable Viability Staining Kit (200 assays)

32002 1kit
EUR 283
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 405/452 Fixable Viability Staining Kit (200 assays)

32003 1kit
EUR 283
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 488/515 Fixable Viability Staining Kit (200 assays)

32004 1kit
EUR 283
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 568/583 Fixable Viability Staining Kit (200 assays)

32005 1kit
EUR 283
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 594/614 Fixable Viability Staining Kit (200 assays)

32006 1kit
EUR 283
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 640/662 Fixable Viability Staining Kit (200 assays)

32007 1kit
EUR 283
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 750/777 Fixable Viability Staining Kit (200 assays)

32008 1kit
EUR 283
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 405/545 Fixable Viability Staining Kit (200 assays)

32009 1kit
EUR 283
Description: Minimum order quantity: 1 unit of 1kit

Cell Navigator™ Lysosome Staining Kit *Deep Red Fluorescence*

22659 500 Tests
EUR 176

Cell Navigator™ Mitochondrion Staining Kit *Deep Red Fluorescence*

22669 500 Assays
EUR 219

Caspase Screening Kit

K2056-25 25 assays
EUR 279

Human Active caspase 3 ELISA kit

E01A0015-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Human Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Active caspase 3 ELISA kit

E01A0015-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Human Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Active caspase 3 ELISA kit

E02A0015-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Active caspase 3 ELISA kit

E02A0015-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Active caspase 3 ELISA kit

E02A0015-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Active caspase 3 ELISA kit

E04A0015-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rabbit Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Active caspase 3 ELISA kit

E04A0015-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rabbit Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Active caspase 3 ELISA kit

E04A0015-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rabbit Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Active caspase 3 ELISA kit

E03A0015-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Mouse Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Active caspase 3 ELISA kit

E03A0015-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Mouse Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Active caspase 3 ELISA kit

E03A0015-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Mouse Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Active caspase 3 ELISA kit

E07A0015-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Porcine Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Active caspase 3 ELISA kit

E07A0015-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Porcine Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Active caspase 3 ELISA kit

E07A0015-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Porcine Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Active caspase 3 ELISA kit

E09A0015-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Monkey Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Active caspase 3 ELISA kit

E09A0015-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Monkey Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Active caspase 3 ELISA kit

E09A0015-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Monkey Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Active caspase 3 ELISA kit

E06A0015-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Goat Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Active caspase 3 ELISA kit

E06A0015-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Goat Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Active caspase 3 ELISA kit

E06A0015-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Goat Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Active caspase 3 ELISA kit

E08A0015-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Canine Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Active caspase 3 ELISA kit

E08A0015-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Canine Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Active caspase 3 ELISA kit

E08A0015-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Canine Active caspase 3 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

JC-1 Mitochondrial Membrane Potential Detection Kit (25 assays)

30001-T 1kit
EUR 127
Description: Minimum order quantity: 1 unit of 1kit

human MMP-9 activity assay kit 96-assays

QZBmmp9H 1 plate 96 assays
EUR 501

mouse MMP-9 activity assay kit 96-assays

QZBmmp9M 1 plate 96 assays
EUR 501

Caspase 9 antibody

70R-49474 100 ul
EUR 244
Description: Purified Polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-49475 100 ul
EUR 244
Description: Purified Polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-51476 100 ul
EUR 244
Description: Purified Polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-33457 100 ug
EUR 327
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-33459 100 ug
EUR 327
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

70R-33461 100 ug
EUR 327
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 Antibody

AF6348 200ul
EUR 304
Description: Caspase 9 Antibody detects endogenous levels of total Caspase 9.

Caspase 9 Antibody

ABF6348 100 ug
EUR 438

anti-Caspase 9

YF-PA23361 50 ul
EUR 334
Description: Mouse polyclonal to Caspase 9

Caspase 9 protein

30R-2406 25 units
EUR 375
Description: Purified recombinant Human Caspase 9 protein

Caspase 9 protein

30R-2407 50 ug
EUR 581
Description: Purified recombinant Human Caspase 9 protein

Caspase-9 Antibody

3016-100
EUR 338

Caspase-9 Antibody

3016-30T
EUR 146

Caspase 9 antibody

20R-1425 100 ug
EUR 673
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

20R-1468 100 ug
EUR 673
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

20R-1472 100 ug
EUR 781
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

20R-1557 100 ug
EUR 673
Description: Rabbit polyclonal Caspase 9 antibody

Caspase 9 antibody

20R-2497 50 ug
EUR 281
Description: Rabbit polyclonal Caspase 9 antibody

The high-speed gentle supply offers, for the primary time, pixel super-resolved imaging of >104 2 μm beads in answer present process Brownian movement, with out vital movement blur. The automated picture processing routines allow the counting of particular person beads and clusters, offering a quantitative sensor readout that relies upon on each bead and analyte concentrations. Fits to the chemical binding idea are offered. For NeutrAvidin, we discover a restrict of detection (LOD) of <27 ng/mL (450 pM) and a dynamic vary of 2-Four orders of magnitude. For mouse interferon-γ, the LOD is <Three ng/mL (200 pM) and the dynamic vary is at the very least Four orders of magnitude. The QLAB sensor holds promise for point-of-care functions in low-resource communities and the place protocol simplicity is necessary.

Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells

Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells

The means of autophagy is a necessary mobile mechanism, required to take care of basic cell well being by the elimination of dysfunctional organelles, such because the ER, peroxisomes and mitochondria, in addition to protein aggregates, and micro organism. Autophagy is an especially dynamic course of, and instruments are continually being developed to check the varied steps of this course of. This protocol particulars a technique to check the top steps of autophagy-lysosomal fusion and the formation of the autolysosome. Many strategies have been used to check the varied steps of the autophagy course of.

Here we describe the RedGreen-assay (RG-assay), an immunofluorescence-based method used to visualise the concentrating on of substrates to the autolysosome in reside cells. This method takes benefit of the low lysosomal pH and over-expression of a tandem GFP-mCherry tagged protein focused to an organelle of curiosity. While within the impartial cytosol or autophagosome, each GFP and RFP will fluoresce. However, inside the autolysosome, the GFP sign is quenched as a result of low pH surroundings and the RFP emission sign will predominate. This method is quickly quantifiable and amenable to excessive throughput experiments. Additionally, by tagging the GFP-RFP tandem fluorescent protein with organelle particular concentrating on sequences, it may be used to measure a variety of substrates of autophagy.

Quantifying the ratio of alternatively spliced mRNA variants of genes with identified various splicing variants is extremely related for many purposes. Herein, we describe the validation of a quantitative PCR design for the simplified quantification of identified mRNA splice variants. The assay makes use of a single-common primer pair, twin probe design for the willpower of splicing variants in a single nicely configuration. We used murine XBP-1 splicing variants, XBP-1S and XBP-1U, to validate and show the efficiency traits of this method. Using artificial XBP-1S and XBP-1U cDNA in addition to cDNA synthesized from mouse beta-cell line MIN6, we established the efficiency parameters and dynamic vary of the assay. Reliable quantification of each variants at various focus gradients was proven.

Platelet Isolation and Activation Assays

Platelets regulate hemostasis and are the important thing determinants of pathogenic thrombosis following atherosclerotic plaque rupture. Platelets flow into in an inactive state, however develop into activated in response to wreck to the endothelium, which exposes thrombogenic materials resembling collagen to the blood circulate. Activation leads to plenty of responses, together with secretion of soluble bioactive molecules through the discharge of alpha and dense granules, activation of membrane adhesion receptors, launch of microparticles, and externalization of phosphatidylserine.

These processes facilitate agency adhesion to websites of damage and the recruitment and activation of different platelets and leukocytes, leading to aggregation and thrombus formation. Platelet activation drives the hemostatic response, and likewise contributes to pathogenic thrombus formation. Thus, quantification of platelet-associated responses is vital to many pathophysiologically related processes. Here we describe protocols for isolating, counting, and activating platelets, and for the fast quantification of cell floor proteins utilizing circulate cytometry.

Extracellular vesicles (EVs) are produced by all domains of life together with Bacteria, Archaea and Eukarya. EVs are important for mobile physiology and comprise assorted cargo: virulence components, cell wall transforming enzymes, extracellular matrix parts and even nucleic acids and metabolites. While varied protocols for isolating EVs have been established for mammalian cells, the sphere is actively growing instruments to check EVs in different organisms.

In this protocol we describe our strategies to carry out density gradient purification of EVs in bacterial cells, permitting for separation of EV subpopulations, adopted by safety assays for EV cargo characterization. Furthermore, we devised a protocol which includes a fluorescent conjugate of fatty acids into EVs, the primary to permit reside-cell EV monitoring to look at launch of EVs, together with throughout an infection of mammalian cells by pathogenic micro organism. These protocols are highly effective instruments for EV researchers as they allow the statement of EV launch and the research of the mechanisms of their formation and launch.

 Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells

Ex vivo Drosophila Wing Imaginal Disc Culture and Furin Inhibitor Assay

Furin is an evolutionarily conserved proprotein convertase (PC) household enzyme with a broad vary of substrates which might be important for developmental, homeostatic, and illness pathways. Classical genetic approaches and in vitro biochemical or cell organic assays recognized that precursor types of most development issue household proteins are processed by Furin. To quantitatively assess the potential function of Furin in cleaving and modulating intercellular dispersion of a Drosophila signaling protein, we developed a easy assay by combining genetics, ex vivo organ tradition, pharmacological remedy, and imaging analyses.

Caspase-9 Colorimetric Assay Kit

K119-200
EUR 620

Caspase-9 Colorimetric Assay Kit

K119-400
EUR 958

Caspase-9 Colorimetric Assay Kit

K2019-100 100 assays
EUR 529

Caspase-9 Colorimetric Assay Kit

K2019-200 200 assays
EUR 696

Caspase-9 Colorimetric Assay Kit

K2019-400 400 assays
EUR 1156

Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (25 assays)

30011-1 1KIT
EUR 204
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-8 Colorimetric Assay Kit

K113-25
EUR 207

Caspase-6 Colorimetric Assay Kit

K115-25
EUR 202

Caspase-2 Colorimetric Assay Kit

K117-25
EUR 213

Caspase-3 Colorimetric Assay Kit

K106-25
EUR 207

Caspase-5 Colorimetric Assay Kit

K123-25
EUR 213

Caspase-10 Colorimetric Assay Kit

K125-25
EUR 213

Caspase-4 Colorimetric Assay Kit

K127-25
EUR 229

Caspase-1 Colorimetric Assay Kit

K111-25
EUR 207

Caspase-3 Colorimetric Assay Kit

K2008-25 25 assays
EUR 238

Caspase-1 Colorimetric Assay Kit

K2011-25 25 assays
EUR 238

Caspase-8 Colorimetric Assay Kit

K2013-25 25 assays
EUR 238

Caspase-6 Colorimetric Assay Kit

K2015-25 25 assays
EUR 238

Caspase-2 Colorimetric Assay Kit

K2017-25 25 assays
EUR 251

Caspase-5 Colorimetric Assay Kit

K2196-25 25 assays
EUR 251

Caspase-10 Colorimetric Assay Kit

K2197-25 25 assays
EUR 251

Caspase-4 Colorimetric Assay Kit

K2199-25 25 assays
EUR 266

Caspase-9 Fluorometric Assay Kit

K118-25
EUR 207

Caspase-9 Fluorometric Assay Kit

K2018-25 25 assays
EUR 238

CASPASE3 DEVDR110 ASSAY (25 ASSAYS)

30008-1 1KIT
EUR 204
Description: Minimum order quantity: 1 unit of 1KIT

Caspase Colorimetric Substrate Set II

K134-9-25
EUR 659

Caspase 9 Assay Kit

20-abx299021
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase 9 Assay Kit

20-abx299035
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests

Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit (100 assays)

30008-2 1KIT
EUR 383
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (100 assays)

30011-2 1KIT
EUR 383
Description: Minimum order quantity: 1 unit of 1KIT

Caspase Colorimetric Substrate Set II Plus

K138-9-25
EUR 833

Caspase 3 Colorimetric Assay Kit

55R-1270 25 assays
EUR 277
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

Caspase 1 Colorimetric Assay Kit

55R-1273 25 assays
EUR 277
Description: Assay Kit for detection of Capase 1 activity in the research laboratory

Caspase 8 Colorimetric Assay Kit

55R-1275 25 assays
EUR 277
Description: Assay Kit for detection of Capase 8 activity in the research laboratory

Caspase 6 Colorimetric Assay Kit

55R-1277 25 assays
EUR 284
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 2 Colorimetric Assay Kit

55R-1279 25 assays
EUR 303
Description: Assay Kit for detection of Capase 2 activity in the research laboratory

Caspase 5 Colorimetric Assay Kit

55R-1283 25 assays
EUR 303
Description: Assay Kit for detection of Capase 5 activity in the research laboratory

Caspase 10 Colorimetric Assay Kit

55R-1285 25 assays
EUR 303
Description: Assay Kit for detection of Capase 10 activity in the research laboratory

Caspase 4 Colorimetric Assay Kit

55R-1287 25 assays
EUR 312
Description: Assay Kit for detection of Capase 4 activity in the research laboratory

Caspase-8 Colorimetric Assay Kit

K113-100
EUR 479

Caspase-8 Colorimetric Assay Kit

K113-200
EUR 620

Caspase-8 Colorimetric Assay Kit

K113-400
EUR 958

Caspase-6 Colorimetric Assay Kit

K115-100
EUR 452

Caspase-6 Colorimetric Assay Kit

K115-200
EUR 615

Caspase-6 Colorimetric Assay Kit

K115-400
EUR 958

Caspase-2 Colorimetric Assay Kit

K117-100
EUR 468

Caspase-2 Colorimetric Assay Kit

K117-200
EUR 615

Caspase-2 Colorimetric Assay Kit

K117-400
EUR 958

Caspase-3 Colorimetric Assay Kit

K106-100
EUR 479

Caspase-3 Colorimetric Assay Kit

K106-200
EUR 631

Caspase-3 Colorimetric Assay Kit

K106-400
EUR 958

Caspase-5 Colorimetric Assay Kit

K123-100
EUR 468

Caspase-5 Colorimetric Assay Kit

K123-200
EUR 615

Caspase-5 Colorimetric Assay Kit

K123-400
EUR 958

Caspase-10 Colorimetric Assay Kit

K125-100
EUR 463

Caspase-10 Colorimetric Assay Kit

K125-200
EUR 615

Caspase-10 Colorimetric Assay Kit

K125-400
EUR 958

Caspase-4 Colorimetric Assay Kit

K127-100
EUR 468

Caspase-4 Colorimetric Assay Kit

K127-200
EUR 615

Caspase-4 Colorimetric Assay Kit

K127-400
EUR 958

Caspase-1 Colorimetric Assay Kit

K111-100
EUR 479

Caspase-1 Colorimetric Assay Kit

K111-200
EUR 620

Caspase-1 Colorimetric Assay Kit

K111-400
EUR 958

Caspase-3 Colorimetric Assay Kit

K2008-100 100 assays
EUR 474

Caspase-3 Colorimetric Assay Kit

K2008-200 200 assays
EUR 696

Caspase-3 Colorimetric Assay Kit

K2008-400 400 assays
EUR 1086

Caspase-1 Colorimetric Assay Kit

K2011-100 100 assays
EUR 474

Caspase-1 Colorimetric Assay Kit

K2011-200 200 assays
EUR 696

Caspase-1 Colorimetric Assay Kit

K2011-400 400 assays
EUR 1086

Caspase-8 Colorimetric Assay Kit

K2013-100 100 assays
EUR 474

Caspase-8 Colorimetric Assay Kit

K2013-200 200 assays
EUR 696

Caspase-8 Colorimetric Assay Kit

K2013-400 400 assays
EUR 1086

Caspase-6 Colorimetric Assay Kit

K2015-100 100 assays
EUR 487

Caspase-6 Colorimetric Assay Kit

K2015-200 200 assays
EUR 696

Caspase-6 Colorimetric Assay Kit

K2015-400 400 assays
EUR 1101

Caspase-2 Colorimetric Assay Kit

K2017-100 100 assays
EUR 529

Caspase-2 Colorimetric Assay Kit

K2017-200 200 assays
EUR 725

Caspase-2 Colorimetric Assay Kit

K2017-400 400 assays
EUR 1156

Caspase-5 Colorimetric Assay Kit

K2196-100 100 assays
EUR 529

Caspase-5 Colorimetric Assay Kit

K2196-200 200 assays
EUR 725

Caspase-5 Colorimetric Assay Kit

K2196-400 400 assays
EUR 1156

Caspase-10 Colorimetric Assay Kit

K2197-100 100 assays
EUR 529

Caspase-10 Colorimetric Assay Kit

K2197-200 200 assays
EUR 738

Caspase-10 Colorimetric Assay Kit

K2197-400 400 assays
EUR 1114

Caspase-4 Colorimetric Assay Kit

K2199-100 100 assays
EUR 557

Caspase-4 Colorimetric Assay Kit

K2199-200 200 assays
EUR 738

Caspase-4 Colorimetric Assay Kit

K2199-400 400 assays
EUR 1114

Caspase 9 Fluorometric Assay Kit

55R-1280 25 assays
EUR 277
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase-9 Fluorometric Assay Kit

K118-100
EUR 468

Caspase-9 Fluorometric Assay Kit

K118-200
EUR 615

Caspase-9 Fluorometric Assay Kit

K118-400
EUR 958

Caspase 9 Assay Kit, green

KF17206 25 Tests
EUR 304

Caspase 9 Assay Kit, red

KF17302 25 Tests
EUR 304

Caspase-9 Fluorometric Assay Kit

K2018-100 100 assays
EUR 502

Caspase-9 Fluorometric Assay Kit

K2018-200 200 assays
EUR 669

Caspase-9 Fluorometric Assay Kit

K2018-400 400 assays
EUR 1114

Caspase-6 Fluorometric Assay Kit

K114-25
EUR 202

Caspase-2 Fluorometric Assay Kit

K116-25
EUR 202

Caspase-3 Fluorometric Assay Kit

K105-25
EUR 207

Caspase-10 Fluorometric Assay Kit

K124-25
EUR 202

Caspase-4 Fluorometric Assay Kit

K126-25
EUR 213

Caspase-12 Fluorometric Assay Kit

K139-25
EUR 256

Caspase-5 Fluorometric Assay Kit

K122-25
EUR 202

Caspase-1 Fluorometric Assay Kit

K110-25
EUR 207

Caspase-8 Fluorometric Assay Kit

K112-25
EUR 207

Caspase-3 Fluorometric Assay Kit

K2007-25 25 assays
EUR 238

Caspase-1 Fluorometric Assay Kit

K2010-25 25 assays
EUR 238

Caspase-8 Fluorometric Assay Kit

K2012-25 25 assays
EUR 238

Caspase-6 Fluorometric Assay Kit

K2014-25 25 assays
EUR 238

Caspase-2 Fluorometric Assay Kit

K2016-25 25 assays
EUR 238

Caspase-12 Fluorometric Assay Kit

K2150-25 25 assays
EUR 251

Caspase-5 Fluorometric Assay Kit

K2195-25 25 assays
EUR 238

Caspase-4 Fluorometric Assay Kit

K2198-25 25 assays
EUR 251

human MMP-9 activity assay kit 96-assays

QZBmmp9H 1 plate 96 assays
EUR 501

mouse MMP-9 activity assay kit 96-assays

QZBmmp9M 1 plate 96 assays
EUR 501

hydroxyproline assay kit 96-assays

QZBhypro1 1 plate 96 assays
EUR 323

Caspase-9, human recombinant

1089-25
EUR 283

Caspase Colorimetric Substrate Set

K132-7-25
EUR 544

Fluorescein Active Caspase-9 Staining Kit

K2051-25 25 assays
EUR 238

Red Active Caspase-9 Staining Kit

K2055-25 25 assays
EUR 238

Caspase 9 Colorimetric Cell-Based ELISA Kit

EKC1089 100ul
EUR 572

Caspase-9, human recombinant protein

E1009-25 25 units
EUR 308
Description: Caspase-9 belongs to the caspase-family of cysteine proteases. Caspase-9 also exists in cells as an inactive proenzyme like other caspases. Procaspase-9 is processed at aspartate residues to form active caspase-9 during the initiation of apoptosis.

Acetylcholinesterase Activity Assay Kit - 100 Assays

AR4001-unit unit
EUR 317

OxiSelect AOPP Assay Kit (200 assays)

STA-318 200 assays
EUR 635
Description: Advanced Oxidation Protein Products (AOPP) are uremic toxins created during oxidative stress through the reaction of plasma proteins with chlorinated oxidants such as chloramines or hypochlorous acid. AOPP levels are elevated in patients with renal complications, diabetes mellitus, and atherosclerosis, as well as HIV-positive patients. Our OxiSelect AOPP Assay Kit provides a convenient method to assess oxidative stress. The kit includes a Chloramine standard and an AOPP Human Serum Albumin conjugate for use as a positive control.

soluble collagen assay kit 96-assays

QZBcol1-1 1 plate 96 assays
EUR 334

hydroxyproline assay kit 2x 96-assays

QZBhypro2 2 plates 192 assays
EUR 590

total collagen assay kit 96-assays

QZBtotcol1 1 plate 96 assays
EUR 334

total protein assay kit 96-assays

QZBtotprot1 1 plate 96 assays
EUR 288

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-25
EUR 240

CaspGLOW? Red Active Caspase-9 Staining Kit

K199-25
EUR 240

Caspase Colorimetric Substrate Set Plus

K136-7-25
EUR 707