Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells

Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells

The means of autophagy is a necessary mobile mechanism, required to take care of basic cell well being by the elimination of dysfunctional organelles, such because the ER, peroxisomes and mitochondria, in addition to protein aggregates, and micro organism. Autophagy is an especially dynamic course of, and instruments are continually being developed to check the varied steps of this course of. This protocol particulars a technique to check the top steps of autophagy-lysosomal fusion and the formation of the autolysosome. Many strategies have been used to check the varied steps of the autophagy course of.

Here we describe the RedGreen-assay (RG-assay), an immunofluorescence-based method used to visualise the concentrating on of substrates to the autolysosome in reside cells. This method takes benefit of the low lysosomal pH and over-expression of a tandem GFP-mCherry tagged protein focused to an organelle of curiosity. While within the impartial cytosol or autophagosome, each GFP and RFP will fluoresce. However, inside the autolysosome, the GFP sign is quenched as a result of low pH surroundings and the RFP emission sign will predominate. This method is quickly quantifiable and amenable to excessive throughput experiments. Additionally, by tagging the GFP-RFP tandem fluorescent protein with organelle particular concentrating on sequences, it may be used to measure a variety of substrates of autophagy.

Quantifying the ratio of alternatively spliced mRNA variants of genes with identified various splicing variants is extremely related for many purposes. Herein, we describe the validation of a quantitative PCR design for the simplified quantification of identified mRNA splice variants. The assay makes use of a single-common primer pair, twin probe design for the willpower of splicing variants in a single nicely configuration. We used murine XBP-1 splicing variants, XBP-1S and XBP-1U, to validate and show the efficiency traits of this method. Using artificial XBP-1S and XBP-1U cDNA in addition to cDNA synthesized from mouse beta-cell line MIN6, we established the efficiency parameters and dynamic vary of the assay. Reliable quantification of each variants at various focus gradients was proven.

Platelet Isolation and Activation Assays

Platelets regulate hemostasis and are the important thing determinants of pathogenic thrombosis following atherosclerotic plaque rupture. Platelets flow into in an inactive state, however develop into activated in response to wreck to the endothelium, which exposes thrombogenic materials resembling collagen to the blood circulate. Activation leads to plenty of responses, together with secretion of soluble bioactive molecules through the discharge of alpha and dense granules, activation of membrane adhesion receptors, launch of microparticles, and externalization of phosphatidylserine.

These processes facilitate agency adhesion to websites of damage and the recruitment and activation of different platelets and leukocytes, leading to aggregation and thrombus formation. Platelet activation drives the hemostatic response, and likewise contributes to pathogenic thrombus formation. Thus, quantification of platelet-associated responses is vital to many pathophysiologically related processes. Here we describe protocols for isolating, counting, and activating platelets, and for the fast quantification of cell floor proteins utilizing circulate cytometry.

Extracellular vesicles (EVs) are produced by all domains of life together with Bacteria, Archaea and Eukarya. EVs are important for mobile physiology and comprise assorted cargo: virulence components, cell wall transforming enzymes, extracellular matrix parts and even nucleic acids and metabolites. While varied protocols for isolating EVs have been established for mammalian cells, the sphere is actively growing instruments to check EVs in different organisms.

In this protocol we describe our strategies to carry out density gradient purification of EVs in bacterial cells, permitting for separation of EV subpopulations, adopted by safety assays for EV cargo characterization. Furthermore, we devised a protocol which includes a fluorescent conjugate of fatty acids into EVs, the primary to permit reside-cell EV monitoring to look at launch of EVs, together with throughout an infection of mammalian cells by pathogenic micro organism. These protocols are highly effective instruments for EV researchers as they allow the statement of EV launch and the research of the mechanisms of their formation and launch.

 Lysosome Targeting RedGreen-assay: Selective Autophagy Sensing Assay for Mammalian Cells

Ex vivo Drosophila Wing Imaginal Disc Culture and Furin Inhibitor Assay

Furin is an evolutionarily conserved proprotein convertase (PC) household enzyme with a broad vary of substrates which might be important for developmental, homeostatic, and illness pathways. Classical genetic approaches and in vitro biochemical or cell organic assays recognized that precursor types of most development issue household proteins are processed by Furin. To quantitatively assess the potential function of Furin in cleaving and modulating intercellular dispersion of a Drosophila signaling protein, we developed a easy assay by combining genetics, ex vivo organ tradition, pharmacological remedy, and imaging analyses.

Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (25 assays)

30011-1 1KIT
EUR 204
Description: Minimum order quantity: 1 unit of 1KIT

Caspase 9 Colorimetric Assay Kit

55R-1281 25 assays
EUR 296
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase-9 Colorimetric Assay Kit

K2019-100 100 assays
EUR 529

Caspase-9 Colorimetric Assay Kit

K2019-200 200 assays
EUR 696

Caspase-9 Colorimetric Assay Kit

K2019-400 400 assays
EUR 1156

Caspase-9 Colorimetric Assay Kit

K119-100
EUR 490

Caspase-9 Colorimetric Assay Kit

K119-200
EUR 620

Caspase-9 Colorimetric Assay Kit

K119-400
EUR 958

Caspase-5 Colorimetric Assay Kit

K2196-25 25 assays
EUR 251

Caspase-10 Colorimetric Assay Kit

K2197-25 25 assays
EUR 251

Caspase-4 Colorimetric Assay Kit

K2199-25 25 assays
EUR 266

Caspase-8 Colorimetric Assay Kit

K2013-25 25 assays
EUR 238

Caspase-6 Colorimetric Assay Kit

K2015-25 25 assays
EUR 238

Caspase-2 Colorimetric Assay Kit

K2017-25 25 assays
EUR 251

Caspase-8 Colorimetric Assay Kit

K113-25
EUR 207

Caspase-6 Colorimetric Assay Kit

K115-25
EUR 202

Caspase-2 Colorimetric Assay Kit

K117-25
EUR 213

Caspase-3 Colorimetric Assay Kit

K2008-25 25 assays
EUR 238

Caspase-1 Colorimetric Assay Kit

K2011-25 25 assays
EUR 238

Caspase-3 Colorimetric Assay Kit

K106-25
EUR 207

Caspase-5 Colorimetric Assay Kit

K123-25
EUR 213

Caspase-10 Colorimetric Assay Kit

K125-25
EUR 213

Caspase-4 Colorimetric Assay Kit

K127-25
EUR 229

Caspase-1 Colorimetric Assay Kit

K111-25
EUR 207

Caspase-9 Fluorometric Assay Kit

K2018-25 25 assays
EUR 238

Caspase-9 Fluorometric Assay Kit

K118-25
EUR 207

CASPASE3 DEVDR110 ASSAY (25 ASSAYS)

30008-1 1KIT
EUR 204
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit (100 assays)

30008-2 1KIT
EUR 383
Description: Minimum order quantity: 1 unit of 1KIT

Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (100 assays)

30011-2 1KIT
EUR 383
Description: Minimum order quantity: 1 unit of 1KIT

Caspase Colorimetric Substrate Set II

K134-9-25
EUR 659

Caspase 9 Assay Kit

20-abx299021
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.

Caspase 9 Assay Kit

20-abx299035
  • EUR 1302.00
  • EUR 582.00
  • 100 tests
  • 25 tests
  • Shipped within 1 week.

Caspase Colorimetric Substrate Set II Plus

K138-9-25
EUR 833

Caspase 3 Colorimetric Assay Kit

55R-1270 25 assays
EUR 277
Description: Assay Kit for detection of Capase 3 activity in the research laboratory

Caspase 1 Colorimetric Assay Kit

55R-1273 25 assays
EUR 277
Description: Assay Kit for detection of Capase 1 activity in the research laboratory

Caspase 8 Colorimetric Assay Kit

55R-1275 25 assays
EUR 277
Description: Assay Kit for detection of Capase 8 activity in the research laboratory

Caspase 6 Colorimetric Assay Kit

55R-1277 25 assays
EUR 284
Description: Assay Kit for detection of Capase 6 activity in the research laboratory

Caspase 2 Colorimetric Assay Kit

55R-1279 25 assays
EUR 303
Description: Assay Kit for detection of Capase 2 activity in the research laboratory

Caspase 5 Colorimetric Assay Kit

55R-1283 25 assays
EUR 303
Description: Assay Kit for detection of Capase 5 activity in the research laboratory

Caspase 10 Colorimetric Assay Kit

55R-1285 25 assays
EUR 303
Description: Assay Kit for detection of Capase 10 activity in the research laboratory

Caspase 4 Colorimetric Assay Kit

55R-1287 25 assays
EUR 312
Description: Assay Kit for detection of Capase 4 activity in the research laboratory

Caspase-5 Colorimetric Assay Kit

K2196-100 100 assays
EUR 529

Caspase-5 Colorimetric Assay Kit

K2196-200 200 assays
EUR 725

Caspase-5 Colorimetric Assay Kit

K2196-400 400 assays
EUR 1156

Caspase-10 Colorimetric Assay Kit

K2197-100 100 assays
EUR 529

Caspase-10 Colorimetric Assay Kit

K2197-200 200 assays
EUR 738

Caspase-10 Colorimetric Assay Kit

K2197-400 400 assays
EUR 1114

Caspase-4 Colorimetric Assay Kit

K2199-100 100 assays
EUR 557

Caspase-4 Colorimetric Assay Kit

K2199-200 200 assays
EUR 738

Caspase-4 Colorimetric Assay Kit

K2199-400 400 assays
EUR 1114

Caspase-8 Colorimetric Assay Kit

K2013-100 100 assays
EUR 474

Caspase-8 Colorimetric Assay Kit

K2013-200 200 assays
EUR 696

Caspase-8 Colorimetric Assay Kit

K2013-400 400 assays
EUR 1086

Caspase-6 Colorimetric Assay Kit

K2015-100 100 assays
EUR 487

Caspase-6 Colorimetric Assay Kit

K2015-200 200 assays
EUR 696

Caspase-6 Colorimetric Assay Kit

K2015-400 400 assays
EUR 1101

Caspase-2 Colorimetric Assay Kit

K2017-100 100 assays
EUR 529

Caspase-2 Colorimetric Assay Kit

K2017-200 200 assays
EUR 725

Caspase-2 Colorimetric Assay Kit

K2017-400 400 assays
EUR 1156

Caspase-8 Colorimetric Assay Kit

K113-100
EUR 479

Caspase-8 Colorimetric Assay Kit

K113-200
EUR 620

Caspase-8 Colorimetric Assay Kit

K113-400
EUR 958

Caspase-6 Colorimetric Assay Kit

K115-100
EUR 452

Caspase-6 Colorimetric Assay Kit

K115-200
EUR 615

Caspase-6 Colorimetric Assay Kit

K115-400
EUR 958

Caspase-2 Colorimetric Assay Kit

K117-100
EUR 468

Caspase-2 Colorimetric Assay Kit

K117-200
EUR 615

Caspase-2 Colorimetric Assay Kit

K117-400
EUR 958

Caspase-3 Colorimetric Assay Kit

K2008-100 100 assays
EUR 474

Caspase-3 Colorimetric Assay Kit

K2008-200 200 assays
EUR 696

Caspase-3 Colorimetric Assay Kit

K2008-400 400 assays
EUR 1086

Caspase-1 Colorimetric Assay Kit

K2011-200 200 assays
EUR 696

Caspase-1 Colorimetric Assay Kit

K2011-400 400 assays
EUR 1086

Caspase-3 Colorimetric Assay Kit

K106-100
EUR 479

Caspase-3 Colorimetric Assay Kit

K106-200
EUR 631

Caspase-3 Colorimetric Assay Kit

K106-400
EUR 958

Caspase-5 Colorimetric Assay Kit

K123-100
EUR 468

Caspase-5 Colorimetric Assay Kit

K123-200
EUR 615

Caspase-5 Colorimetric Assay Kit

K123-400
EUR 958

Caspase-10 Colorimetric Assay Kit

K125-100
EUR 463

Caspase-10 Colorimetric Assay Kit

K125-200
EUR 615

Caspase-10 Colorimetric Assay Kit

K125-400
EUR 958

Caspase-4 Colorimetric Assay Kit

K127-100
EUR 468

Caspase-4 Colorimetric Assay Kit

K127-200
EUR 615

Caspase-4 Colorimetric Assay Kit

K127-400
EUR 958

Caspase-1 Colorimetric Assay Kit

K111-100
EUR 479

Caspase-1 Colorimetric Assay Kit

K111-200
EUR 620

Caspase-1 Colorimetric Assay Kit

K111-400
EUR 958

Caspase 9 Fluorometric Assay Kit

55R-1280 25 assays
EUR 277
Description: Assay Kit for detection of Capase 9 activity in the research laboratory

Caspase 9 Assay Kit, green

KF17206 25 Tests
EUR 304

Caspase 9 Assay Kit, red

KF17302 25 Tests
EUR 304

Caspase-9 Fluorometric Assay Kit

K2018-100 100 assays
EUR 502

Caspase-9 Fluorometric Assay Kit

K2018-200 200 assays
EUR 669

Caspase-9 Fluorometric Assay Kit

K2018-400 400 assays
EUR 1114

Caspase-9 Fluorometric Assay Kit

K118-100
EUR 468

Caspase-9 Fluorometric Assay Kit

K118-200
EUR 615

Caspase-9 Fluorometric Assay Kit

K118-400
EUR 958

human MMP-9 activity assay kit 96-assays

QZBmmp9H 1 plate 96 assays
EUR 501
The protocol herein describes easy methods to ex vivo tradition Drosophila wing imaginal discs expressing a fluorescently tagged Drosophila Fibroblast Growth Factor (FGF, Branchless/Bnl) over an extended time period within the presence of Furin inhibitors and monitor the cleavage and intercellular dispersion of the truncated Bnl components utilizing microscopy. Although the assay described right here is for assessing the impact of Furin inhibition on Bnl cleavage within the Drosophila larval wing imaginal disc, the precept and methodology can simply be adopted for every other alerts, tissue techniques, or organisms. This technique and protocol present an assay for analyzing Furin exercise on a particular substrate by straight visualizing the spatiotemporal distribution of its truncated components in an ex vivo-cultured organ.

A Highly Sensitive, Reproducible Assay for Determining 4-hydroxynonenal Protein Adducts in Biological Material

A Highly Sensitive, Reproducible Assay for Determining 4-hydroxynonenal Protein Adducts in Biological Material

Oxidative stress is related to quite a few illnesses, and markers of oxidative stress in organic materials have gotten a mainstay of each experimental and scientific/epidemiological analysis. Lipid peroxidation is a serious type of oxidative stress, however resulting from their speedy degradation and instability, lipid peroxides are notoriously tough to measure, significantly in organic specimens the place their manufacturing and removing are constantly occuring. Thus, a generally used surrogate marker of lipid peroxidation is protein adducts of 4-Hydroxynonenal (HNE), an α, β-unsaturated hydroxyalkenal (i.e., a reactive aldehyde) fashioned through degradation of oxidized polyunsaturated fatty acids (PUFAs).

HNE adducts will be measured through commercially-available immunosorbent assays, however these have their limitations resulting from extreme prices, and reproducibility amongst laboratories is difficult resulting from variability in assay sensitivity, process, and reagents. Here we current a reproducible, facile, and economically conservative protocol for quantifying HNE protein adducts. The key to this protocol is to generate HNE-adduct requirements by incubating bovine serum albumin (BSA) with HNE. These requirements are then adsorbed to immunsorbent plastic in a multi-well plate format alongside organic samples.

An enzyme-linked immunosorbent assay (ELISA) is then carried out on the multi-well plate utilizing commercially-available major and secondary antibodies, and a peroxide-based fluorescent growing reagent. This protocol is very delicate and provides benefits to business sources in that it permits for reproducible, high-throughput quantitation of HNE adducts in a lot of samples. As such, it might be helpful as a biomarker of power oxidative stress for experimental and scientific research.

Understanding the molecular mechanism governing the higher-order regulation of actin dynamics requires chemically-defined and quantitative assays. Recently, the membrane reworking giant GTPase, dynamin, has been recognized as a brand new actin cross-linking molecule. Dynamin regulates actin cytoskeleton via binding to, self-assembling round, and aligning them into actin bundles. Here we make the most of dynamin for example and current a easy protocol to investigate the actin bundling exercise in vitro. This protocol particulars the strategy for F-actin reconstitution in addition to quantitative and qualitative analyses for actin bundling exercise of dynamins. Measurement of the actin bundling exercise of different actin-binding proteins might also be utilized to this protocol with acceptable changes relying on the protein of curiosity.

Genetic identification of medicinally used Salacia species by nrDNA ITS sequences and a PCR-RFLP assay for authentication of Salacia-related well being meals

The roots and stems of a number of Salacia species have been used as conventional medicines, particularly in Ayurvedic medical system for the therapy of diabetes, rheumatism, gonorrhea, amenorrhea, pores and skin illnesses, and so forth. Due to reported proof supporting Salacia’s useful results in early-stage diabetes and different lifestyle-related illnesses, Salacia-based dietary dietary supplements and well being meals have been gaining reputation in Japan and different nations in current years. However, because of the morphological similarities between Salacia crops, significantly in the medicinally used elements (roots and stems), the authentication of the botanical identities of Salacia-derived merchandise is difficult. This research goals to develop a genetic strategy to authenticate the medicinally used Salacia species and to find out the botanical sources of the commercially obtainable Salacia-derived merchandise.
 The sequences of nuclear DNA inside transcribed spacer (ITS) and chloroplast trnK-rps16 area had been decided and in contrast between 10 plant specimens from three medicinally used Salacia species in addition to 48 samples of business crude medicine. Moreover, a PCR-restriction fragment size polymorphism (RFLP) assay was developed for speedy identification primarily based on the ITS sequences. The plant specimens from the three medicinally used Salacia species confirmed three foremost kinds of sequences in each ITS (sorts I, II, III) and trnK-rps16 (i, ii, iii) areas.
Combined the sequences of ITS and trnK-rps16 areas, S. reticulata and S. oblonga had sort I-i and kind III-iii or comparable sequences, respectively. S. chinensis had sort II-ii or II(536M)-i sequences. Forty-eight samples of business crude medicine had been recognized primarily based on ITS and trnK-rps16 DNA barcode. A handy PCR-RFLP assay utilizing Cac8I restriction enzyme was established and utilized to determine the botanical sources of well being meals merchandise bought from on-line retailers. All the twelve samples had been recognized as S. chinensis.
The nrDNA ITS sequences offered helpful info to authenticate Salacia species and to elucidate the phylogenetic relationship inside the Salacia genus. Genetic identification outcomes revealed that S. chinensis and S. reticulate are the most important sources of commercially obtainable Salacia-products. Based on the ITS sequences, a handy PCR-RFLP assay was established for the identification of the medicinally used Salacia species in addition to their derived well being meals merchandise.
 A Highly Sensitive, Reproducible Assay for Determining 4-hydroxynonenal Protein Adducts in Biological Material

The Ring-Pull Assay for Mechanical Properties of Fibrous Soft Tissues – An Analysis of the Uniaxial Approximation and a Correction for Nonlinear Thick-Walled Tissues

The ring-pull check, the place a hoop of tissue is hooked through two pins and stretched, is a well-liked biomechanical check, particularly for small arteries. Although handy and dependable, the ring check produces inhomogeneous pressure, making willpower of fabric parameters non-trivial. To decide correction elements between ring-pull-estimated and true tissue properties.  A finite-element mannequin of ring pulling was constructed for a pattern with nonlinear, anisotropic mechanical conduct typical of arteries. The pin power and pattern cross-section had been used to compute an obvious modulus at small and huge pressure, which had been in comparison with the desired properties. The ensuing corrections had been validated with experiments on porcine and ovine arteries.

Acid Phosphatase Activity Fluorometric Assay Kit

K421-500
EUR 349

Alkaline Phosphatase Activity Colorimetric Assay Kit

K412-500
EUR 539

Alkaline Phosphatase Activity Colorimetric Assay Kit

K2075-500 500 assays
EUR 544

StemTAG Alkaline Phosphatase Staining and Activity Assay Kit, Fluorometric

CBA-308 2 x 100 assays
EUR 705
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Combo Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining as well as in a 96-well plate with either colorimetric or fluorescence detection.

Alkaline Phosphatase Activity Assay Kit

55R-1402 500 assays
EUR 740
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory

Alkaline Phosphatase Activity Assay Kit

55R-1406 500 assays
EUR 438
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory

Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric)

K829-100
EUR 533

StemTAG Alkaline Phosphatase Activity Assay Kit, Colorimetric

CBA-301 100 assays
EUR 403
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Kits provide reagents for quantifying alkaline phosphatase activity in a convenient 96-well plate format, with either colorimetric or fluorescence detection.

Acetylcholinesterase Activity Assay Kit - 100 Assays

AR4001-unit unit
EUR 317

Neprilysin Activity Assay Kit (Fluorometric)

K487-100
EUR 610

Chitotriosidase Activity Assay Kit (Fluorometric)

K512-100
EUR 620

?-Glucuronidase Activity Assay Kit (Fluorometric)

K514-100
EUR 620

Methyltransferase Activity Assay Kit (Fluorometric)

K521-100
EUR 566

Sphingomyelinase Activity Fluorometric Assay Kit

K574-100
EUR 457

DPP4 Activity Fluorometric Assay Kit

K779-100
EUR 544

Protease Activity Fluorometric Assay Kit

K781-100
EUR 387

Renin Activity Fluorometric Assay Kit

K800-100
EUR 523

Lipoxygenase Activity Assay Kit (Fluorometric)

K978-100
EUR 653

?-Xylosidase Activity Assay Kit (Fluorometric)

K981-100
EUR 588

Urokinase Activity Fluorometric Assay Kit

K728-100
EUR 544

Neuraminidase Activity Fluorometric Assay Kit

K732-100
EUR 523

Uricase Activity Assay Kit (Fluorometric)

K734-100
EUR 512

Deubiquitinase Activity Assay Kit (Fluorometric)

K485-100
EUR 533

HDAC6 Activity Assay Kit (Fluorometric)

K466-100
EUR 631

DPP4 Activity Fluorometric Assay Kit

K2178-100 100 assays
EUR 557

GST Fluorometric Activity Assay Kit

K260-100
EUR 468

Chymotrypsin Activity Assay Kit (Fluorometric)

K352-100
EUR 490

Calpain Activity Fluorometric Assay Kit

K2062-100 100 assays
EUR 627

HAT Activity Fluorometric Assay Kit

K334-100
EUR 539

HDAC3 Activity Fluorometric Assay Kit

K343-100
EUR 479

Sirtuin Activity Assay Kit (Fluorometric)

K324-100
EUR 572

HDAC Activity Fluorometric Assay Kit

K330-100
EUR 490

Plasmin Activity Assay Kit (Fluorometric)

K381-100
EUR 457

Thrombin Activity Fluorometric Assay Kit

K373-100
EUR 697

Calpain Activity Fluorometric Assay Kit

K240-100
EUR 588

Proteasome Activity Fluorometric Assay Kit

K245-100
EUR 479

Lysozyme Activity Assay Kit (Fluorometric)

K236-100
EUR 490

Neuraminidase Activity Fluorometric Assay Kit

K2230-100 100 assays
EUR 599

HDAC8 Activity Fluorometric Assay Kit

K348-100
EUR 490

PLTP Activity Fluorometric Assay Kit

K2087-100 100 assays
EUR 669

CETP Activity Fluorometric Assay Kit

K2089-100 100 assays
EUR 725

Proteasome Activity Fluorometric Assay Kit

K2096-100 100 assays
EUR 502

GST Fluorometric Activity Assay Kit

K2105-100 100 assays
EUR 502

CD38 Activity Assay Kit (Fluorometric)

K2042-100 100 assays
EUR 581

Sialyltransferase Activity Assay Kit (Fluorometric)

K2048-100 100 assays
EUR 601

Acid Phosphatase Activity Colorimetric Assay Kit

K411-500
EUR 359

Diamine Oxidase Activity Assay Kit (Fluorometric)

K496-100
EUR 566

Oxalate Oxidase Activity Assay Kit (Fluorometric)

K509-100
EUR 479

?-L-Fucosidase Activity Assay Kit (Fluorometric)

K542-100
EUR 523

Cyclooxygenase (COX) Activity Assay Kit (Fluorometric)

K549-100
EUR 419

PLTP Activity Fluorometric Assay Kit II

K593-100
EUR 588

CETP Activity Fluorometric Assay Kit II

K595-100
EUR 642

Asparaginase Activity Colorimetric/Fluorometric Assay Kit

K754-100
EUR 550

PicoProbe? Hexokinase Activity Assay Kit (Fluorometric)

K769-100
EUR 637

Peroxidase Activity Colorimetric/Fluorometric Assay Kit

K772-100
EUR 387

Catalase Activity Colorimetric/Fluorometric Assay Kit

K773-100
EUR 419

MMP-3 Activity Fluorometric Assay Kit

K783-100
EUR 512

Rat Renin Activity Fluorometric Assay Kit

K806-100
EUR 648

Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit

K807-100
EUR 865

TEV Protease Activity Assay Kit (Fluorometric)

K842-100
EUR 501

Total Phosphodiesterase Activity Assay Kit (Fluorometric)

K927-100
EUR 615

Lysyl Oxidase Activity Assay Kit (Fluorometric)

K928-100
EUR 533

PicoProbeTM Glucokinase Activity Assay Kit (Fluorometric)

K969-100
EUR 593

Aromatase (CYP19A) Activity Assay Kit (Fluorometric)

K983-100
EUR 620

Cystathionine ? Synthase Activity Assay Kit (Fluorometric)

K998-100
EUR 620

Myeloperoxidase (MPO) Fluorometric Activity Assay Kit

K745-100
EUR 550

Enteropeptidase/Enterokinase Activity Fluorometric Assay Kit

K758-100
EUR 610

Enolase Activity Colorimetric/Fluorometric Assay Kit

K691-100
EUR 794

Lipoprotein Lipase Activity Fluorometric Assay Kit

K721-100
EUR 539

Lipase Activity Fluorometric Assay Kit III

K724-100
EUR 533

Myeloperoxidase (MPO) Fluorometric Activity Assay Kit

K2169-100 100 assays
EUR 572

Catalase Activity Colorimetric/Fluorometric Assay Kit

K2177-100 100 assays
EUR 418

Pepsin/Pepsinogen Activity Assay Kit (Fluorometric)

K446-100
EUR 468

Cytidine Deaminase Activity Assay Kit (Fluorometric)

K451-100
EUR 468

DNAse I Activity Assay Kit (Fluorometric)

K429-100
EUR 697

Factor VIIIa Activity Assay Kit (Fluorometric)

K358-100
EUR 539

HAT (H4) Activity Fluorometric Assay Kit

K336-100
EUR 501

Adenosine Deaminase Activity Assay Kit (Fluorometric)

K328-100
EUR 457

InSitu HDAC Activity Fluorometric Assay Kit

K339-100
EUR 479

Neutrophil Elastase Activity Assay kit (Fluorometric)

K383-100
EUR 419

Beta-Secretase Activity Fluorometric Assay Kit

K360-100
EUR 620

Factor Xa Activity Fluorometric Assay Kit

K361-100
EUR 620

?-Secretase (BACE1) Activity Assay Kit (Fluorometric)

K388-100
EUR 604

Factor IXa Activity Assay Kit (Fluorometric)

K364-100
EUR 490

Phospholipase A2 Activity Assay Kit (Fluorometric)

K400-100
EUR 566

Cathepsin B Activity Fluorometric Assay Kit

K2151-100 100 assays
EUR 516

Cathepsin K Activity Fluorometric Assay Kit

K2152-100 100 assays
EUR 516

Cathepsin L Activity Fluorometric Assay Kit

K2153-100 100 assays
EUR 516

Cathepsin D Activity Fluorometric Assay Kit

K2154-100 100 assays
EUR 516

Cathepsin S Activity Fluorometric Assay Kit

K2155-100 100 assays
EUR 516

Cathepsin E Activity Assay Kit (Fluorometric)

K165-100
EUR 637

Granzyme B Activity Fluorometric Assay Kit

K168-100
EUR 533

Granzyme B Activity Fluorometric Assay Kit

K2001-100 100 assays
EUR 586

Lipase Activity Fluorometric Assay Kit III

K2095-100 100 assays
EUR 586

Cathepsin B Activity Fluorometric Assay Kit

K140-100
EUR 512

Cathepsin K Activity Fluorometric Assay Kit

K141-100
EUR 512

Cathepsin L Activity Fluorometric Assay Kit

K142-100
EUR 512
The correction was additional utilized to experiments on mouse aortic rings to find out materials and failure properties. Calculating pressure primarily based on centerline stretch somewhat than inner-wall or outer-wall stretch afforded higher estimation of tissue properties. Additional correction elements had been developed primarily based on ring wall thickness H, centerline ring radius R c , and pin radius a. The corrected estimates for tissue properties had been in good settlement with uniaxial stretch experiments.

Gentamicin Protection Assay to Determine the Number of Intracellular Bacteria during Infection of Human TC7 Intestinal Epithelial Cells by Shigella flexneri

Gentamicin Protection Assay to Determine the Number of Intracellular Bacteria during Infection of Human TC7 Intestinal Epithelial Cells by Shigella flexneri

Shigella flexneri is an intracellular bacterial pathogen that positive aspects entry to the intestine epithelium utilizing a specialised Type III Secretion System (T3SS). Various determinants mediating this invasive an infection have been experimentally verified utilizing the classical gentamicin safety assay introduced right here. In this assay epithelial cell strains are contaminated by micro organism in vitro and the extracellular micro organism are killed by gentamicin. The internalized micro organism, that are shielded from the bactericidal motion of gentamicin, are recovered by lysing the epithelial cells and enumerated by figuring out the colonies fashioned on strong medium.

Various methods primarily based on gentle microscopy, reminiscent of immunofluorescence and micro organism expressing fluorescent proteins, are additionally used for finding out intracellular micro organism. However, these methods should not solely labor intensive and require refined tools, however principally are additionally not quantitative. Despite being a straightforward quantitative technique to research invasiveness of micro organism, the gentamicin safety assay can’t distinguish between the survival and multiplication of the internalized micro organism over longer incubation durations.

To alleviate the problems created by multiplication and dissemination of internalized micro organism, complementary assays like plaque formation assays are required. This protocol presents a straightforward and cost-effective technique to decide the invasiveness and the capability to set up an an infection of Shigella below totally different circumstances. Automatic and visible interpretation of outcome bands have been additionally in contrast for the immunochromatography-based BinaxNOW and ImmuView UATs.

Urinary antigen exams (UATs) are sometimes used to diagnose Legionnaires’ illness as they’re speedy and simple to carry out on readily obtainable urine samples with out the want for specialised abilities in contrast to standard strategies. Recently developed automated readers for UATs could present goal outcomes interpretation, particularly in instances of weak outcome bands. Using 53 outlined affected person urine samples, we evaluated the efficiency of the BinaxNOW Legionella Antigen Card (Abbott), ImmuView S. pneumoniae and Legionella (SSI Diagnostica), STANDARD F Legionella Ag FIA (SD Biosensor), and Sofia Legionella FIA (Quidel) concurrently with their respective automated readers.

SARS-CoV-2 lateral circulation assays for attainable use in nationwide covid-19 seroprevalence surveys (React 2): diagnostic accuracy research

ensitivity analyses have been carried out on sera saved from 320 earlier contributors in the React 2 programme with confirmed earlier extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2) an infection. Specificity analyses have been carried out on 1000 prepandemic serum samples. 100 new contributors with confirmed earlier SARS-CoV-2 an infection attended research clinics for finger prick testing.  Laboratory sensitivity and specificity analyses have been carried out for seven LFIAs on a minimal of 200 serum samples from contributors with confirmed SARS-CoV-2 an infection and 500 prepandemic serum samples, respectively.
Three LFIAs have been discovered to have a laboratory sensitivity superior to the finger prick sensitivity of the LFIA presently utilized in React 2 seroprevalence research (84%). These LFIAs have been then additional evaluated by way of finger prick testing on contributors with confirmed earlier SARS-CoV-2 an infection: two LFIAs (Surescreen, Panbio) have been evaluated in clinics in June-July 2020 and the third LFIA (AbC-19) in September 2020. A spike protein enzyme linked immunoassay and hybrid double antigen binding assay have been used as laboratory reference requirements.
The accuracy of LFIAs in detecting immunoglobulin G (IgG) antibodies to SARS-CoV-2 in contrast with two reference requirements. The sensitivity and specificity of seven new LFIAs that have been analysed utilizing sera diverse from 69% to 100%, and from 98.6% to 100%, respectively (in contrast with the two reference requirements). Sensitivity on finger prick testing was 77% (95% confidence interval 61.4% to 88.2%) for Panbio, 86% (72.7% to 94.8%) for Surescreen, and 69% (53.8% to 81.3%) for AbC-19 in contrast with the reference requirements. Sensitivity for sera from matched medical samples carried out on AbC-19 was considerably larger with serum than finger prick at 92% (80.0% to 97.7%, P=0.01). Antibody titres diverse significantly amongst cohorts. The numbers of constructive samples recognized by finger prick in the lowest antibody titre quarter diverse amongst LFIAs.
Gentamicin Protection Assay to Determine the Number of Intracellular Bacteria during Infection of Human TC7 Intestinal Epithelial Cells by Shigella flexneri

Establishment of Novel Protein Interaction Assays between Sin3 and REST Using Surface Plasmon Resonance and Time-Resolved Fluorescence Energy Transfer

Repressor element-1 (RE-1) or neural restrictive silencer component (NRSE) certain with a zinc finger transcription repressor, RE-1 silencing transcription issue (REST, also called neural restrictive silencer issue, NRSF) has been recognized as a basic repressor component in lots of genes, together with neuronal genes. Genes regulated by REST/NRSF regulate multifaceted neuronal phenotypes, and their defects in the equipment trigger neuropathies, issues of neuron exercise), autism and so forth. In REST repressions, the N-terminal repressor area recruits Sin3B by way of its paired amphipathic helix 1 (PAH1) area, which performs an necessary function as a scaffold for histone deacetylase 1 and a pair of. This equipment has a important function in sustaining neuronal robustness.

Acetylcholinesterase Activity Assay Kit - 100 Assays

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In this research, so as to set up protein-protein interplay assays mimicking a binding floor between Sin3B and REST, we chosen necessary amino acids from structural data of the PAH1/REST complicated after which tried to reconstitute it utilizing recombinant quick peptides derived from PAH1/REST. Initially, we validated whether or not biotinylated REST interacts with glutathione S-transferase (GST)-tagged PAH1 and whether or not one other PAH1 peptide (PAH1-FLAG) competitively binds with biotinylated REST utilizing floor plasmon resonance (SPR). We noticed a direct interplay and aggressive binding of two PAH1 peptides.

Secondly, so as to set up a high-throughput and high-dynamic-range assay, we utilized an simply carried out novel time-resolved fluorescence vitality switch (TR-FRET) assay, and intently monitored this interplay. Finally, we succeeded in establishing a novel high-quality TR-FRET assay and a novel interplay assay primarily based on SPR.