Oxidative stress is related to quite a few illnesses, and markers of oxidative stress in organic materials have gotten a mainstay of each experimental and scientific/epidemiological analysis. Lipid peroxidation is a serious type of oxidative stress, however resulting from their speedy degradation and instability, lipid peroxides are notoriously tough to measure, significantly in organic specimens the place their manufacturing and removing are constantly occuring. Thus, a generally used surrogate marker of lipid peroxidation is protein adducts of 4-Hydroxynonenal (HNE), an α, β-unsaturated hydroxyalkenal (i.e., a reactive aldehyde) fashioned through degradation of oxidized polyunsaturated fatty acids (PUFAs).
HNE adducts will be measured through commercially-available immunosorbent assays, however these have their limitations resulting from extreme prices, and reproducibility amongst laboratories is difficult resulting from variability in assay sensitivity, process, and reagents. Here we current a reproducible, facile, and economically conservative protocol for quantifying HNE protein adducts. The key to this protocol is to generate HNE-adduct requirements by incubating bovine serum albumin (BSA) with HNE. These requirements are then adsorbed to immunsorbent plastic in a multi-well plate format alongside organic samples.
An enzyme-linked immunosorbent assay (ELISA) is then carried out on the multi-well plate utilizing commercially-available major and secondary antibodies, and a peroxide-based fluorescent growing reagent. This protocol is very delicate and provides benefits to business sources in that it permits for reproducible, high-throughput quantitation of HNE adducts in a lot of samples. As such, it might be helpful as a biomarker of power oxidative stress for experimental and scientific research.
Understanding the molecular mechanism governing the higher-order regulation of actin dynamics requires chemically-defined and quantitative assays. Recently, the membrane reworking giant GTPase, dynamin, has been recognized as a brand new actin cross-linking molecule. Dynamin regulates actin cytoskeleton via binding to, self-assembling round, and aligning them into actin bundles. Here we make the most of dynamin for example and current a easy protocol to investigate the actin bundling exercise in vitro. This protocol particulars the strategy for F-actin reconstitution in addition to quantitative and qualitative analyses for actin bundling exercise of dynamins. Measurement of the actin bundling exercise of different actin-binding proteins might also be utilized to this protocol with acceptable changes relying on the protein of curiosity.
Genetic identification of medicinally used Salacia species by nrDNA ITS sequences and a PCR-RFLP assay for authentication of Salacia-related well being meals
The roots and stems of a number of Salacia species have been used as conventional medicines, particularly in Ayurvedic medical system for the therapy of diabetes, rheumatism, gonorrhea, amenorrhea, pores and skin illnesses, and so forth. Due to reported proof supporting Salacia’s useful results in early-stage diabetes and different lifestyle-related illnesses, Salacia-based dietary dietary supplements and well being meals have been gaining reputation in Japan and different nations in current years. However, because of the morphological similarities between Salacia crops, significantly in the medicinally used elements (roots and stems), the authentication of the botanical identities of Salacia-derived merchandise is difficult. This research goals to develop a genetic strategy to authenticate the medicinally used Salacia species and to find out the botanical sources of the commercially obtainable Salacia-derived merchandise.
The sequences of nuclear DNA inside transcribed spacer (ITS) and chloroplast trnK-rps16 area had been decided and in contrast between 10 plant specimens from three medicinally used Salacia species in addition to 48 samples of business crude medicine. Moreover, a PCR-restriction fragment size polymorphism (RFLP) assay was developed for speedy identification primarily based on the ITS sequences. The plant specimens from the three medicinally used Salacia species confirmed three foremost kinds of sequences in each ITS (sorts I, II, III) and trnK-rps16 (i, ii, iii) areas.
Combined the sequences of ITS and trnK-rps16 areas, S. reticulata and S. oblonga had sort I-i and kind III-iii or comparable sequences, respectively. S. chinensis had sort II-ii or II(536M)-i sequences. Forty-eight samples of business crude medicine had been recognized primarily based on ITS and trnK-rps16 DNA barcode. A handy PCR-RFLP assay utilizing Cac8I restriction enzyme was established and utilized to determine the botanical sources of well being meals merchandise bought from on-line retailers. All the twelve samples had been recognized as S. chinensis.
The nrDNA ITS sequences offered helpful info to authenticate Salacia species and to elucidate the phylogenetic relationship inside the Salacia genus. Genetic identification outcomes revealed that S. chinensis and S. reticulate are the most important sources of commercially obtainable Salacia-products. Based on the ITS sequences, a handy PCR-RFLP assay was established for the identification of the medicinally used Salacia species in addition to their derived well being meals merchandise.
The Ring-Pull Assay for Mechanical Properties of Fibrous Soft Tissues – An Analysis of the Uniaxial Approximation and a Correction for Nonlinear Thick-Walled Tissues
The ring-pull check, the place a hoop of tissue is hooked through two pins and stretched, is a well-liked biomechanical check, particularly for small arteries. Although handy and dependable, the ring check produces inhomogeneous pressure, making willpower of fabric parameters non-trivial. To decide correction elements between ring-pull-estimated and true tissue properties. A finite-element mannequin of ring pulling was constructed for a pattern with nonlinear, anisotropic mechanical conduct typical of arteries. The pin power and pattern cross-section had been used to compute an obvious modulus at small and huge pressure, which had been in comparison with the desired properties. The ensuing corrections had been validated with experiments on porcine and ovine arteries.
StemTAG Alkaline Phosphatase
Staining and Activity Assay Kit, Fluorometric |
CBA-308 |
Cell Biolabs |
2 x 100 assays |
EUR 705 |
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Combo Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining as well as in a 96-well plate with either colorimetric or fluorescence detection. |
Alkaline Phosphatase Activity Assay Kit |
55R-1402 |
Fitzgerald |
500 assays |
EUR 740 |
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory |
Alkaline Phosphatase Activity Assay Kit |
55R-1406 |
Fitzgerald |
500 assays |
EUR 438 |
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory |
Protein Tyrosine Phosphatase Activity Assay Kit (Fluorometric) |
K829-100 |
Biovision |
|
EUR 533 |
StemTAG Alkaline Phosphatase Activity Assay Kit, Colorimetric |
CBA-301 |
Cell Biolabs |
100 assays |
EUR 403 |
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Kits provide reagents for quantifying alkaline phosphatase activity in a convenient 96-well plate format, with either colorimetric or fluorescence detection. |
Neprilysin Activity Assay Kit (Fluorometric) |
K487-100 |
Biovision |
|
EUR 610 |
Chitotriosidase Activity Assay Kit (Fluorometric) |
K512-100 |
Biovision |
|
EUR 620 |
?-Glucuronidase Activity Assay Kit (Fluorometric) |
K514-100 |
Biovision |
|
EUR 620 |
Methyltransferase Activity Assay Kit (Fluorometric) |
K521-100 |
Biovision |
|
EUR 566 |
Sphingomyelinase Activity Fluorometric Assay Kit |
K574-100 |
Biovision |
|
EUR 457 |
DPP4 Activity Fluorometric Assay Kit |
K779-100 |
Biovision |
|
EUR 544 |
Protease Activity Fluorometric Assay Kit |
K781-100 |
Biovision |
|
EUR 387 |
Renin Activity Fluorometric Assay Kit |
K800-100 |
Biovision |
|
EUR 523 |
Lipoxygenase Activity Assay Kit (Fluorometric) |
K978-100 |
Biovision |
|
EUR 653 |
?-Xylosidase Activity Assay Kit (Fluorometric) |
K981-100 |
Biovision |
|
EUR 588 |
Urokinase Activity Fluorometric Assay Kit |
K728-100 |
Biovision |
|
EUR 544 |
Neuraminidase Activity Fluorometric Assay Kit |
K732-100 |
Biovision |
|
EUR 523 |
Uricase Activity Assay Kit (Fluorometric) |
K734-100 |
Biovision |
|
EUR 512 |
Deubiquitinase Activity Assay Kit (Fluorometric) |
K485-100 |
Biovision |
|
EUR 533 |
HDAC6 Activity Assay Kit (Fluorometric) |
K466-100 |
Biovision |
|
EUR 631 |
GST Fluorometric Activity Assay Kit |
K260-100 |
Biovision |
|
EUR 468 |
Chymotrypsin Activity Assay Kit (Fluorometric) |
K352-100 |
Biovision |
|
EUR 490 |
HAT Activity Fluorometric Assay Kit |
K334-100 |
Biovision |
|
EUR 539 |
HDAC3 Activity Fluorometric Assay Kit |
K343-100 |
Biovision |
|
EUR 479 |
Sirtuin Activity Assay Kit (Fluorometric) |
K324-100 |
Biovision |
|
EUR 572 |
HDAC Activity Fluorometric Assay Kit |
K330-100 |
Biovision |
|
EUR 490 |
Plasmin Activity Assay Kit (Fluorometric) |
K381-100 |
Biovision |
|
EUR 457 |
Thrombin Activity Fluorometric Assay Kit |
K373-100 |
Biovision |
|
EUR 697 |
Calpain Activity Fluorometric Assay Kit |
K240-100 |
Biovision |
|
EUR 588 |
Proteasome Activity Fluorometric Assay Kit |
K245-100 |
Biovision |
|
EUR 479 |
Lysozyme Activity Assay Kit (Fluorometric) |
K236-100 |
Biovision |
|
EUR 490 |
HDAC8 Activity Fluorometric Assay Kit |
K348-100 |
Biovision |
|
EUR 490 |
HDAC Activity Fluorometric Assay Kit |
K2031-100 |
ApexBio |
100 assays |
EUR 502 |
HAT Activity Fluorometric Assay Kit |
K2034-100 |
ApexBio |
100 assays |
EUR 557 |
Calpain Activity Fluorometric Assay Kit |
K2062-100 |
ApexBio |
100 assays |
EUR 627 |
PLTP Activity Fluorometric Assay Kit |
K2087-100 |
ApexBio |
100 assays |
EUR 669 |
CETP Activity Fluorometric Assay Kit |
K2089-100 |
ApexBio |
100 assays |
EUR 725 |
Proteasome Activity Fluorometric Assay Kit |
K2096-100 |
ApexBio |
100 assays |
EUR 502 |
GST Fluorometric Activity Assay Kit |
K2105-100 |
ApexBio |
100 assays |
EUR 502 |
DPP4 Activity Fluorometric Assay Kit |
K2178-100 |
ApexBio |
100 assays |
EUR 557 |
Neuraminidase Activity Fluorometric Assay Kit |
K2230-100 |
ApexBio |
100 assays |
EUR 599 |
Acetylcholinesterase Activity Assay Kit - 100 Assays |
AR4001-unit |
BosterBio |
unit |
EUR 317 |
Acid Phosphatase Activity Colorimetric Assay Kit |
K411-500 |
Biovision |
|
EUR 359 |
Alkaline Phosphatase Assay Kit |
Z5030033 |
Biochain |
250 assays |
EUR 660 |
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Alkaline Phosphatase Assay Kit |
abx096002-100Assays |
Abbexa |
100 Assays |
EUR 316 |
|
Alkaline Phosphatase Assay Kit |
abx098406-Hitachi7060R190ml2R245ml1 |
Abbexa |
Hitachi 7060; R1: 90ml×2 R2: 45ml×1 |
EUR 222 |
|
Alkaline Phosphatase Assay Kit |
abx098406-Hitachi7170R132ml4R28ml4 |
Abbexa |
Hitachi 7170; R1: 32ml×4 R2: 8ml×4 |
EUR 222 |
|
Alkaline Phosphatase Assay Kit |
abx098406-Toshiba120R150ml4R250ml1 |
Abbexa |
Toshiba 120; R1: 50ml×4 R2: 50ml×1 |
EUR 222 |
|
Alkaline Phosphatase Assay Kit |
abx098406-Toshiba40R150ml4R250ml1 |
Abbexa |
Toshiba 40; R1: 50ml×4 R2: 50ml×1 |
EUR 222 |
|
Beta-Mannosidase Activity Assay Kit (Fluorometric) |
K2045-100 |
Biovision |
100 assays |
EUR 551 |
Diamine Oxidase Activity Assay Kit (Fluorometric) |
K496-100 |
Biovision |
|
EUR 566 |
Oxalate Oxidase Activity Assay Kit (Fluorometric) |
K509-100 |
Biovision |
|
EUR 479 |
?-L-Fucosidase Activity Assay Kit (Fluorometric) |
K542-100 |
Biovision |
|
EUR 523 |
Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) |
K549-100 |
Biovision |
|
EUR 419 |
PLTP Activity Fluorometric Assay Kit II |
K593-100 |
Biovision |
|
EUR 588 |
CETP Activity Fluorometric Assay Kit II |
K595-100 |
Biovision |
|
EUR 642 |
Asparaginase Activity Colorimetric/Fluorometric Assay Kit |
K754-100 |
Biovision |
|
EUR 550 |
PicoProbe? Hexokinase Activity Assay Kit (Fluorometric) |
K769-100 |
Biovision |
|
EUR 637 |
Peroxidase Activity Colorimetric/Fluorometric Assay Kit |
K772-100 |
Biovision |
|
EUR 387 |
Catalase Activity Colorimetric/Fluorometric Assay Kit |
K773-100 |
Biovision |
|
EUR 419 |
MMP-3 Activity Fluorometric Assay Kit |
K783-100 |
Biovision |
|
EUR 512 |
Rat Renin Activity Fluorometric Assay Kit |
K806-100 |
Biovision |
|
EUR 648 |
Adenosylhomocysteinase (AHCY) Activity Fluorometric Assay Kit |
K807-100 |
Biovision |
|
EUR 865 |
TEV Protease Activity Assay Kit (Fluorometric) |
K842-100 |
Biovision |
|
EUR 501 |
Total Phosphodiesterase Activity Assay Kit (Fluorometric) |
K927-100 |
Biovision |
|
EUR 615 |
Lysyl Oxidase Activity Assay Kit (Fluorometric) |
K928-100 |
Biovision |
|
EUR 533 |
PicoProbeTM Glucokinase Activity Assay Kit (Fluorometric) |
K969-100 |
Biovision |
|
EUR 593 |
Aromatase (CYP19A) Activity Assay Kit (Fluorometric) |
K983-100 |
Biovision |
|
EUR 620 |
Cystathionine ? Synthase Activity Assay Kit (Fluorometric) |
K998-100 |
Biovision |
|
EUR 620 |
Myeloperoxidase (MPO) Fluorometric Activity Assay Kit |
K745-100 |
Biovision |
|
EUR 550 |
Enteropeptidase/Enterokinase Activity Fluorometric Assay Kit |
K758-100 |
Biovision |
|
EUR 610 |
Enolase Activity Colorimetric/Fluorometric Assay Kit |
K691-100 |
Biovision |
|
EUR 794 |
Lipoprotein Lipase Activity Fluorometric Assay Kit |
K721-100 |
Biovision |
|
EUR 539 |
Lipase Activity Fluorometric Assay Kit III |
K724-100 |
Biovision |
|
EUR 533 |
Pepsin/Pepsinogen Activity Assay Kit (Fluorometric) |
K446-100 |
Biovision |
|
EUR 468 |
Cytidine Deaminase Activity Assay Kit (Fluorometric) |
K451-100 |
Biovision |
|
EUR 468 |
DNAse I Activity Assay Kit (Fluorometric) |
K429-100 |
Biovision |
|
EUR 697 |
Factor VIIIa Activity Assay Kit (Fluorometric) |
K358-100 |
Biovision |
|
EUR 539 |
HAT (H4) Activity Fluorometric Assay Kit |
K336-100 |
Biovision |
|
EUR 501 |
Adenosine Deaminase Activity Assay Kit (Fluorometric) |
K328-100 |
Biovision |
|
EUR 457 |
InSitu HDAC Activity Fluorometric Assay Kit |
K339-100 |
Biovision |
|
EUR 479 |
Neutrophil Elastase Activity Assay kit (Fluorometric) |
K383-100 |
Biovision |
|
EUR 419 |
Beta-Secretase Activity Fluorometric Assay Kit |
K360-100 |
Biovision |
|
EUR 620 |
Factor Xa Activity Fluorometric Assay Kit |
K361-100 |
Biovision |
|
EUR 620 |
?-Secretase (BACE1) Activity Assay Kit (Fluorometric) |
K388-100 |
Biovision |
|
EUR 604 |
Factor IXa Activity Assay Kit (Fluorometric) |
K364-100 |
Biovision |
|
EUR 490 |
Phospholipase A2 Activity Assay Kit (Fluorometric) |
K400-100 |
Biovision |
|
EUR 566 |
Cathepsin E Activity Assay Kit (Fluorometric) |
K165-100 |
Biovision |
|
EUR 637 |
Granzyme B Activity Fluorometric Assay Kit |
K168-100 |
Biovision |
|
EUR 533 |
Cathepsin B Activity Fluorometric Assay Kit |
K140-100 |
Biovision |
|
EUR 512 |
Cathepsin K Activity Fluorometric Assay Kit |
K141-100 |
Biovision |
|
EUR 512 |
Cathepsin L Activity Fluorometric Assay Kit |
K142-100 |
Biovision |
|
EUR 512 |
Cathepsin D Activity Fluorometric Assay Kit |
K143-100 |
Biovision |
|
EUR 512 |
Cathepsin S Activity Fluorometric Assay Kit |
K144-100 |
Biovision |
|
EUR 512 |
Cathepsin H Activity Fluorometric Assay Kit |
K145-100 |
Biovision |
|
EUR 479 |
OxiSelect Catalase Activity Assay Kit, Fluorometric |
STA-339 |
Cell Biolabs |
500 assays |
EUR 757 |
Description: Catalase is a ubiquitous enzyme that destroys hydrogen peroxides formed during oxidative stress. Our OxiSelect Catalase Activity Assay Kit measures catalase activity in less than one hour from a variety of samples including blood, cells and tissues. Each kit provides sufficient reagents to perform up to 500 assays in a 96-well microtiter plate. This includes blanks, catalase standards and unknown samples. The OxiSelect Catalase Activity Assay Kit (Fluorometric) provides a 40-fold increase in sensitivity compared to our colorimetric assay. |
Granzyme B Activity Fluorometric Assay Kit |
K2001-100 |
ApexBio |
100 assays |
EUR 586 |
Beta-Secretase Activity Fluorometric Assay Kit |
K2043-100 |
ApexBio |
100 assays |
EUR 641 |
Lipase Activity Fluorometric Assay Kit III |
K2095-100 |
ApexBio |
100 assays |
EUR 586 |
Cathepsin B Activity Fluorometric Assay Kit |
K2151-100 |
ApexBio |
100 assays |
EUR 516 |
Cathepsin K Activity Fluorometric Assay Kit |
K2152-100 |
ApexBio |
100 assays |
EUR 516 |
Cathepsin L Activity Fluorometric Assay Kit |
K2153-100 |
ApexBio |
100 assays |
EUR 516 |
Cathepsin D Activity Fluorometric Assay Kit |
K2154-100 |
ApexBio |
100 assays |
EUR 516 |
Cathepsin S Activity Fluorometric Assay Kit |
K2155-100 |
ApexBio |
100 assays |
EUR 516 |
Myeloperoxidase (MPO) Fluorometric Activity Assay Kit |
K2169-100 |
ApexBio |
100 assays |
EUR 572 |
Catalase Activity Colorimetric/Fluorometric Assay Kit |
K2177-100 |
ApexBio |
100 assays |
EUR 418 |
StemTAG Alkaline Phosphatase Staining and Activity Assay Kit, Colorimetric |
CBA-302 |
Cell Biolabs |
2 x 100 assays |
EUR 641 |
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Combo Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining as well as in a 96-well plate with either colorimetric or fluorescence detection. |
Acid Phosphatase Activity Assay Kit |
55R-1405 |
Fitzgerald |
500 assays |
EUR 438 |
Description: Assay Kit for detection of Acid Phosphatase in the research laboratory |
Acid Phosphatase Activity Assay Kit |
55R-1866 |
Fitzgerald |
500 assays |
EUR 484 |
Description: Assay Kit for detection of Acid Phosphatase in the research laboratory |
Alkaline Sphingomyelinase Activity Assay Kit (Colorimetric) |
K987-100 |
Biovision |
|
EUR 588 |
Soluble Epoxide Hydrolase Activity Assay Kit (Fluorometric) |
K477-100 |
Biovision |
|
EUR 566 |
PicoProbeTM Tyrosine Aminotransferase Activity Assay Kit (Fluorometric) |
K486-100 |
Biovision |
|
EUR 566 |
Choline Kinase (ChoK) Activity Assay Kit (Fluorometric) |
K476-100 |
Biovision |
|
EUR 947 |
Ethanolamine Kinase (ETNK) Activity Assay Kit (Fluorometric) |
K498-100 |
Biovision |
|
EUR 533 |
Caspase-3 Activity Assay Kit II (Fluorometric) |
K533-100 |
Biovision |
|
EUR 512 |
Leucine Aminopeptidase (LAP) Activity Assay Kit (Fluorometric) |
K534-100 |
Biovision |
|
EUR 533 |
PicoProbe? Malate Dehydrogenase Activity Assay Kit (Fluorometric) |
K538-100 |
Biovision |
|
EUR 566 |
Monoacylglycerol Lipase (MAGL) Activity Assay Kit (Fluorometric) |
K561-100 |
Biovision |
|
EUR 533 |
PicoProbe? 15-PGDH Activity Assay Kit (Fluorometric) |
K562-100 |
Biovision |
|
EUR 533 |
Purine Nucleoside Phosphorylase Activity Assay Kit (Fluorometric) |
K767-100 |
Biovision |
|
EUR 762 |
Glucose Oxidase Activity Colorimetric/Fluorometric Assay Kit |
K788-100 |
Biovision |
|
EUR 539 |
Beta Galactosidase (?-Gal) Activity Assay Kit (Fluorometric) |
K821-100 |
Biovision |
|
EUR 501 |
HIV-1 Protease Activity Assay Kit (Fluorometric) |
K825-100 |
Biovision |
|
EUR 566 |
HIV-2 Protease Activity Assay Kit (Fluorometric) |
K845-100 |
Biovision |
|
EUR 588 |
RNase Activity Detection/Quantification Assay Kit (Fluorometric) |
K934-100 |
Biovision |
|
EUR 479 |
Paraoxonase 1 (PON1) Activity Assay Kit (Fluorometric) |
K999-100 |
Biovision |
|
EUR 620 |
PicoProbe? Aldehyde Dehydrogenase Activity Fluorometric Assay Kit |
K741-100 |
Biovision |
|
EUR 572 |
UGT Activity Assay / Ligand Screening Kit (Fluorometric) |
K692-100 |
Biovision |
|
EUR 490 |
Pyruvate Kinase Activity Colorimetric/Fluorometric Assay Kit |
K709-100 |
Biovision |
|
EUR 610 |
Xanthine Oxidase Activity Colorimetric/Fluorometric Assay Kit |
K710-100 |
Biovision |
|
EUR 408 |
PicoProbeTM Glutaminase (GLS) Activity Assay Kit (Fluorometric) |
K455-100 |