Evaluation of in vivo potencies performs an necessary function in drug discovery. Traditionally, the mobile exercise and p.c of plasma protein binding of a take a look at agent are evaluated individually, with the plasma protein binding-adjusted mobile efficiency computation used to estimate in vivo efficiency. This course of is expensive, takes weeks to finish, and is more and more unreliable for compounds that bind extensively to plasma proteins. The expression of PD-L1 was considerably elevated on LDGs. Pretreatment of LDGs with anti-PD-L1 antibody considerably counteracted the influence of LDGs on T-SPOT. Treatment of PBMCs with anti-PD-L1 antibody resulted in comparable ISCs with that of LDG removing.
Described in this text is an easy, high-throughput human plasma in-cell Western (ICW) assay that instantly incorporates plasma protein binding right into a mobile pharmacodynamic assay to supply a speedy and correct estimate of in vivo potencies. The assay is flexible and will be readily employed for numerous targets that require quick therapy intervals for displaying maximal organic responses. © 2021 Wiley Periodicals LLC. Basic Protocol: Concentration-dependent human plasma ICW assay to find out take a look at compound IC50 in opposition to the goal of curiosity.
The correlations between the frequencies of LDGs and the outcomes of T-SPOT have been analyzed. T-SPOT with LDG-removed PBMCs and PBMCs with exogenous addition of LDGs have been carried out. The attainable mechanism was explored by detecting the degrees of destructive immune regulatory molecules on LDGs. The influence of programmed loss of life ligand 1 (PD-L1) on T-SPOT was evaluated and confirmed by operate blocking with neutralizing antibody. The optimistic charges of T-SPOT and ISCs in tuberculosis sufferers with low LDGs frequency (n = 22) have been considerably larger than these with excessive LDGs frequency (n = 39). Removal or exogenous addition of LDGs considerably elevated or decreased the ISCs and the optimistic fee of T-SPOT. The frequencies of interferon-γ-producing T cells have been negatively correlated with the frequencies of LDGs.
Analytical and medical efficiency of molecular assay utilized by the Brazilian public laboratory community to detect and discriminate Zika, Dengue and Chikungunya viruses in blood
In response to the Zika epidemics in Brazil, the ZDC molecular assay (Bio-Manguinhos) was developed and registered on the Brazilian Regulatory Agency of Health Surveillance – ANVISA. The circulation of Zika (ZIKV) Dengue (DENV) and Chikungunya (CHIKV) viruses and their medical similarities are challenges to appropriately diagnose these viruses. The simultaneous detection of ZIKV, DENV and CHIKV is a crucial software for analysis and surveillance. Here, we current the analytical and medical efficiency analysis of ZDC molecular assay (Bio-Manguinhos) on the public well being laboratories three years after its registration at ANVISA.
The medical efficiency demonstrates the ZDC molecular assay (Bio-Manguinhos) has 100% sensitivity and 100% specificity to detect and discriminate ZIKV, CHIKV, and DENV from medical plasma samples. The ZDC molecular assay (Bio-Manguinhos) outcomes have been extremely reproducible and no cross-reactivity was seen throughout testing with a panel of different infectious brokers. In conclusion, the ZDC molecular assay (Bio-Manguinhos) is an correct and dependable software to observe Zika, dengue and chikungunya infections in nations like Brazil with simultaneous circulation of the three viruses.
Cell entry of the pandemic virus SARS-CoV-2 is mediated by its spike protein S. As most important antigenic determinant, S protein is in focus of assorted therapeutic methods. Besides particle-cell fusion, S mediates fusion between contaminated and uninfected cells ensuing in syncytia formation. Here we current delicate assay techniques with a excessive dynamic vary and excessive signal-to-noise ratios overlaying not solely particle-cell and cell-cell fusion, but additionally fusion-from-without (FFWO).
In FFWO, S-containing viral particles induce syncytia independently of de novo synthesis of S. Neutralizing antibodies in addition to sera from convalescent sufferers inhibited particle-cell fusion with excessive effectivity. Cell-cell fusion, in distinction, was solely reasonably inhibited regardless of requiring ranges of S protein under the detection restrict of movement cytometry and Western blot. The information point out that syncytia formation as pathological consequence throughout Covid-19 can proceed at low ranges of S protein and is probably not successfully prevented by antibodies. However, reference ranges with VerifyNow have been reported primarily for clopidogrel and there has not but been any research particularly carried out to supply the anticipated on therapy reference ranges following administration of prasugrel and ticagrelor.
Use of the VerifyNow level of care assay to evaluate the pharmacodynamic results of loading and upkeep dose regimens of prasugrel and ticagrelor
Prasugrel and ticagrelor are potent oral platelet P2Y12 inhibitors and are really useful over clopidogrel in sufferers with acute coronary syndrome (ACS). Oral platelet P2Y12 inhibitors are characterised by various levels of pharmacodynamic response profiles as assessed by quite a lot of commercially accessible assays. Because of its ease of use, speedy turnaround occasions and talent to supply outcomes particular to P2Y12 inhibitory results, VerifyNow has emerged as one of the vital generally utilized platelet operate assays.
 Nitric Oxide Colorimetric Assay Kit |
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| 55R-1352 | Fitzgerald | 200 assays | EUR 689 |
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Description: Assay Kit for detection of Nitric Oxide activity in the research laboratory |
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 Nitric Oxide Fluorometric Assay Kit |
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| K252-200 | Biovision | EUR 490 | |
Nitric Oxide Fluorometric Assay Kit |
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| K2099-200 | ApexBio | 200 assays | EUR 502 |
 Nitric Oxide Assay Kit |
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| Z5030031 | Biochain | 100 assays | EUR 743 |
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Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
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Nitric Oxide Assay Kit |
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| 55R-1344 | Fitzgerald | 200 assays | EUR 673 |
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Description: Assay Kit for activity of Nitric Oxide in the research laboratory |
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Nitric Oxide Assay Kit |
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| abx298829-100Assays | Abbexa | 100 Assays | EUR 410 |
 Activity Assay Kit (Colorimetric)) Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric) |
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| K205-100 | Biovision | EUR 512 | |
) OxiSelect AOPP Assay Kit (200 assays) |
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| STA-318 | Cell Biolabs | 200 assays | EUR 635 |
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Description: Advanced Oxidation Protein Products (AOPP) are uremic toxins created during oxidative stress through the reaction of plasma proteins with chlorinated oxidants such as chloramines or hypochlorous acid. AOPP levels are elevated in patients with renal complications, diabetes mellitus, and atherosclerosis, as well as HIV-positive patients. Our OxiSelect AOPP Assay Kit provides a convenient method to assess oxidative stress. The kit includes a Chloramine standard and an AOPP Human Serum Albumin conjugate for use as a positive control. |
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 QuantiChrom Nitric Oxide Assay Kit |
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| D2NO-100 | BioAssay Systems | 100 | EUR 329 |
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Description: Quantitative determination of nitric oxide by colorimetric (540nm) method. Procedure: 40 min. Kit size: 100 tests. Detection limit: 0.6 µM. Shelf life: 6 months. Shipping: ambient temp; storage: 4°C. |
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 RealQuant Nitric Oxide Assay Kit |
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| N0100-025 | GenDepot | 250 Assays | EUR 928 |
 Assay Kit (Colorimetric)) OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit (Colorimetric) |
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| STA-802 | Cell Biolabs | 100 assays | EUR 432 |
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Description: Cell Biolabs? OxiSelect Nitric Oxide (Nitrite/Nitrate) Assay Kit is a simple, colorimetric assay that quantitatively measures NO in various samples by NO2-/NO3- determination. First, the nitrate (NO3-) in the sample is converted to nitrite (NO2-) by nitrate reductase enzyme. Next, total nitrite is detected with Griess Reagents as a colored azo dye product (absorbance 540 nm). Each kit provides sufficient reagents to perform up to 100 assays using a 96-well microtiter plate format, including blanks, standards and unknown samples. The kit is suitable for serum, plasma, urine, saliva, lysates, and media (see Preparation of Samples) with detection sensitivity limit of ~ 2 µM (in 50 µL sample volume). |
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OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit (Colorimetric) |
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| STA-802-5 | Cell Biolabs | 5 x 100 assays | EUR 1726 |
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Description: Cell Biolabs? OxiSelect Nitric Oxide (Nitrite/Nitrate) Assay Kit is a simple, colorimetric assay that quantitatively measures NO in various samples by NO2-/NO3- determination. First, the nitrate (NO3-) in the sample is converted to nitrite (NO2-) by nitrate reductase enzyme. Next, total nitrite is detected with Griess Reagents as a colored azo dye product (absorbance 540 nm). Each kit provides sufficient reagents to perform up to 100 assays using a 96-well microtiter plate format, including blanks, standards and unknown samples. The kit is suitable for serum, plasma, urine, saliva, lysates, and media (see Preparation of Samples) with detection sensitivity limit of ~ 2 µM (in 50 µL sample volume). |
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 Hemoglobin Colorimetric Assay Kit |
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| K219-200 | Biovision | EUR 381 | |
 EnzyChrom Nitric Oxide Synthase Assay Kit |
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| ENOS-100 | BioAssay Systems | 100 | EUR 448 |
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Description: Quantitative determination of nitric oxide synthase activity by colorimetric (540nm) method. Procedure: 40 min. Kit size: 100 tests. Detection limit: 0.25 U/L. Shelf life: 3 months. Shipping: on ice; storage: -20 & 4°C. |
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 enzyme assay, colorimetric micro assay kit, 96 test, Quantitative) Nitric Oxide Synthase (NOS) enzyme assay, colorimetric micro assay kit, 96 test, Quantitative |
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| 1290-NOS-1 | Alpha Diagnostics | 1 kit | EUR 651 |
) Phenolic Compounds Assay Kit (Colorimetric) |
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| K527-200 | Biovision | EUR 550 | |
 Caspase-8 Colorimetric Assay Kit |
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| K113-200 | Biovision | EUR 620 | |
 Caspase-6 Colorimetric Assay Kit |
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| K115-200 | Biovision | EUR 615 | |
 Caspase-2 Colorimetric Assay Kit |
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| K117-200 | Biovision | EUR 615 | |
 Caspase-3 Colorimetric Assay Kit |
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| K106-200 | Biovision | EUR 631 | |
 Caspase-5 Colorimetric Assay Kit |
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| K123-200 | Biovision | EUR 615 | |
 Caspase-10 Colorimetric Assay Kit |
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| K125-200 | Biovision | EUR 615 | |
 Caspase-4 Colorimetric Assay Kit |
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| K127-200 | Biovision | EUR 615 | |
 Caspase-9 Colorimetric Assay Kit |
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| K119-200 | Biovision | EUR 620 | |
 Caspase-1 Colorimetric Assay Kit |
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| K111-200 | Biovision | EUR 620 | |
Caspase-3 Colorimetric Assay Kit |
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| K2008-200 | ApexBio | 200 assays | EUR 696 |
Caspase-1 Colorimetric Assay Kit |
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| K2011-200 | ApexBio | 200 assays | EUR 696 |
Caspase-8 Colorimetric Assay Kit |
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| K2013-200 | ApexBio | 200 assays | EUR 696 |
Caspase-6 Colorimetric Assay Kit |
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| K2015-200 | ApexBio | 200 assays | EUR 696 |
Caspase-2 Colorimetric Assay Kit |
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| K2017-200 | ApexBio | 200 assays | EUR 725 |
Caspase-9 Colorimetric Assay Kit |
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| K2019-200 | ApexBio | 200 assays | EUR 696 |
Caspase-5 Colorimetric Assay Kit |
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| K2196-200 | ApexBio | 200 assays | EUR 725 |
Caspase-10 Colorimetric Assay Kit |
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| K2197-200 | ApexBio | 200 assays | EUR 738 |
Caspase-4 Colorimetric Assay Kit |
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| K2199-200 | ApexBio | 200 assays | EUR 738 |
) Nitric Oxide Colorimteric Detection kit (Two Plates) |
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| K023-H1 | Arbor Assays | 2 x 96 well plate | EUR 273 |
 Nitric Oxide Cell-Based HTS Assay Kit |
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| K979-100 | Biovision | EUR 653 | |
 colorimetric micro assay kit, 96 test, Quantitative) Nitric Oxide (NO/nitrate/nitrite) colorimetric micro assay kit, 96 test, Quantitative |
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| 1280-NOX-1 | Alpha Diagnostics | 1 kit | EUR 529 |
) ATP-GloTM Bioluminometric Cell Viability Assay Kit (200 assays) |
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| 30020-1 | Biotium | 1KIT | EUR 190 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 Glutathione Reductase Activity Colorimetric Assay Kit |
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| K761-200 | Biovision | EUR 512 | |
 Hydrogen Peroxide Colorimetric/Fluorometric Assay Kit |
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| K265-200 | Biovision | EUR 381 | |
Glutathione Reductase Activity Colorimetric Assay Kit |
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| K2173-200 | ApexBio | 200 assays | EUR 516 |
) Glutathione Colorimetric Detection kit (200 High Cuvettes) |
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| K006-H1C-H | Arbor Assays | 200 Cuvette | EUR 471 |
) Glutathione Colorimetric Detection kit (200 Low Cuvettes) |
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| K006-H1C-L | Arbor Assays | 200 Cuvette | EUR 471 |
 Activity Assay Kit (Fluorometric)) Nitric Oxide Synthase (NOS) Activity Assay Kit (Fluorometric) |
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| K206-100 | Biovision | EUR 512 | |
 Assay Kit (Fluorometric)) OxiSelect Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric) |
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| STA-800 | Cell Biolabs | 96 assays | EUR 490 |
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Description: The OxiSelect Intracellular Nitric Oxide Assay Kit is a cell-based assay for rapid detection of intracellular NO or NOS activity in cultured cells. In brief, the cell-permeant NO probe passively diffuses into cells and is deacetylated by cellular esterases to a non-fluorescent intermediate. When intracellular nitric oxide encounters this intermediate, it rapidly oxidizes to a highly fluorescent, triazolo-fluorescein analog. The fluorescence intensity is proportional to the NO levels within the cell cytosol (detection limit of ~3 nM). The kit?s provided Nitric Oxide Fluorometric Probe is NO specific, has excellent photostablity and pH stability, and is detected with standard fluorescein filters. The probe is suitable for flow cytometry, fluorescent microscopy, and fluorescent microplate detection. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, standards, and unknown samples. |
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OxiSelect Intracellular Nitric Oxide (NO) Assay Kit (Fluorometric) |
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| STA-800-5 | Cell Biolabs | 5 x 96 assays | EUR 1923 |
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Description: The OxiSelect Intracellular Nitric Oxide Assay Kit is a cell-based assay for rapid detection of intracellular NO or NOS activity in cultured cells. In brief, the cell-permeant NO probe passively diffuses into cells and is deacetylated by cellular esterases to a non-fluorescent intermediate. When intracellular nitric oxide encounters this intermediate, it rapidly oxidizes to a highly fluorescent, triazolo-fluorescein analog. The fluorescence intensity is proportional to the NO levels within the cell cytosol (detection limit of ~3 nM). The kit?s provided Nitric Oxide Fluorometric Probe is NO specific, has excellent photostablity and pH stability, and is detected with standard fluorescein filters. The probe is suitable for flow cytometry, fluorescent microscopy, and fluorescent microplate detection. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, standards, and unknown samples. |
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 Assay Kit (Colorimetric)) Ferric Reducing Antioxidant Power (FRAP) Assay Kit (Colorimetric) |
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| K515-200 | Biovision | EUR 550 | |
 hydroxyproline assay kit 96-assays |
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| QZBhypro1 | Quickzyme | 1 plate 96 assays | EUR 323 |
 Nitric Oxide Detection Kit |
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| SKT-212-96 | Stressmarq | 2 plates of 96 wells | EUR 342 |
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Description: Indirect Colorimetric assay used for quantitative measuring Nitrate and Nitrite present in a variety of samples in Serum, Plasma, Urine, Saliva, Water, Buffer, Cell Lysates, Tissue Culture Media samples from all species |
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 Nitric oxide generation kit |
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| 00239 | Biotium | 1SET | EUR 311 |
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Description: Minimum order quantity: 1 unit of 1SET |
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 AccuOrangeâ„¢ Protein Quantitation Kit, 200 assays |
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| 30071-T | Biotium | 1KIT | EUR 101 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 Activity Assay Kit) Amplite™ Fluorimetric Extracellular Nitric Oxide (NO) Activity Assay Kit |
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| 16365 | AAT Bioquest | 100 Tests | EUR 350 |
 Assay Kit (Fluorometric)) OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit (Fluorometric) |
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| STA-801 | Cell Biolabs | 100 assays | EUR 473 |
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Description: Cell Biolabs? OxiSelect Nitric Oxide (Nitrite/Nitrate) Assay Kit is a simple, colorimetric assay that quantitatively measures NO in various samples by NO2-/NO3- determination. First, the nitrate (NO3-) in the sample is converted to nitrite (NO2-) by nitrate reductase enzyme. Next, total nitrite is detected with Griess Reagents as a colored azo dye product (absorbance 540 nm). Each kit provides sufficient reagents to perform up to 100 assays using a 96-well microtiter plate format, including blanks, standards and unknown samples. The kit is suitable for serum, plasma, urine, saliva, lysates, and media (see Preparation of Samples) with detection sensitivity limit of ~ 2 µM (in 50 µL sample volume). |
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OxiSelect In Vitro Nitric Oxide (Nitrite / Nitrate) Assay Kit (Fluorometric) |
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| STA-801-5 | Cell Biolabs | 5 x 100 assays | EUR 1819 |
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Description: Cell Biolabs? OxiSelect Nitric Oxide (Nitrite/Nitrate) Assay Kit is a simple, colorimetric assay that quantitatively measures NO in various samples by NO2-/NO3- determination. First, the nitrate (NO3-) in the sample is converted to nitrite (NO2-) by nitrate reductase enzyme. Next, total nitrite is detected with Griess Reagents as a colored azo dye product (absorbance 540 nm). Each kit provides sufficient reagents to perform up to 100 assays using a 96-well microtiter plate format, including blanks, standards and unknown samples. The kit is suitable for serum, plasma, urine, saliva, lysates, and media (see Preparation of Samples) with detection sensitivity limit of ~ 2 µM (in 50 µL sample volume). |
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) Caspase-3 DEVD-R110 Fluorometric and Colorimetric Assay Kit (100 assays) |
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| 30008-2 | Biotium | 1KIT | EUR 383 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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) Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (25 assays) |
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| 30011-1 | Biotium | 1KIT | EUR 204 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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) Caspase-8 IETD-R110 Fluorometric and Colorimetric Assay Kit (100 assays) |
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| 30011-2 | Biotium | 1KIT | EUR 383 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 Acetylcholinesterase Activity Assay Kit - 100 Assays |
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| AR4001-unit | BosterBio | unit | EUR 317 |
 soluble collagen assay kit 96-assays |
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| QZBcol1-1 | Quickzyme | 1 plate 96 assays | EUR 334 |
 hydroxyproline assay kit 2x 96-assays |
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| QZBhypro2 | Quickzyme | 2 plates 192 assays | EUR 590 |
 total collagen assay kit 96-assays |
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| QZBtotcol1 | Quickzyme | 1 plate 96 assays | EUR 334 |
 total protein assay kit 96-assays |
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| QZBtotprot1 | Quickzyme | 1 plate 96 assays | EUR 288 |
 Live Bacterial Gram Stain Kit - 200 assays |
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| 32000-1 | Biotium | 1kit | EUR 190 |
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Description: Minimum order quantity: 1 unit of 1kit |
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) CASPASE3 DEVDR110 ASSAY (25 ASSAYS) |
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| 30008-1 | Biotium | 1KIT | EUR 204 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 Sulfate Assay Kit |
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| K415-200 | Biovision | EUR 414 | |
 Glutathione Colorimetric Assay Kit |
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| 55R-1351 | Fitzgerald | 100 assays | EUR 706 |
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Description: Assay Kit for detection of Glutathione activity in the research laboratory |
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) Methylglyoxal Assay Kit (Colorimetric) |
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| K500-100 | Biovision | EUR 528 | |
) Salicylate Assay Kit (Colorimetric) |
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| K494-100 | Biovision | EUR 544 | |
 Cobalt Colorimetric Assay Kit |
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| K505-100 | Biovision | EUR 457 | |
) Histamine Assay Kit (Colorimetric) |
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| K506-100 | Biovision | EUR 501 | |
 Nickel Colorimetric Assay Kit |
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| K510-100 | Biovision | EUR 457 | |
 Chloride Colorimetric Assay Kit |
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| K530-100 | Biovision | EUR 359 | |
) Lithium Assay Kit (Colorimetric) |
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| K545-100 | Biovision | EUR 620 | |
 Hydroxyproline Colorimetric Assay Kit |
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| K555-100 | Biovision | EUR 599 | |
 Glutamine Colorimetric Assay Kit |
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| K556-100 | Biovision | EUR 588 | |
 Tyrosine Colorimetric Assay Kit |
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| K573-100 | Biovision | EUR 550 | |
 Formate Colorimetric Assay Kit |
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| K653-100 | Biovision | EUR 582 | |
 Isocitrate Colorimetric Assay Kit |
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| K656-100 | Biovision | EUR 474 | |
 Acetate Colorimetric Assay Kit |
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| K658-100 | Biovision | EUR 512 | |
 Phosphoglucomutase Colorimetric Assay Kit |
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| K774-100 | Biovision | EUR 615 | |
 Hexokinase Colorimetric Assay Kit |
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| K789-100 | Biovision | EUR 599 | |
) Methanol Assay Kit (Colorimetric) |
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| K898-100 | Biovision | EUR 664 | |
) Taurine Assay Kit (Colorimetric) |
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| K988-100 | Biovision | EUR 648 | |
 Chitosan Colorimetric Assay Kit |
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| K995-100 | Biovision | EUR 566 | |
) Asparagine Assay Kit (Colorimetric) |
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| K736-100 | Biovision | EUR 610 | |
) Phenylalanine Assay Kit (Colorimetric) |
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| K481-100 | Biovision | EUR 468 | |
 Acetoacetate Colorimetric Assay Kit |
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| K650-100 | Biovision | EUR 773 | |
 Malate Colorimetric Assay Kit |
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| K637-100 | Biovision | EUR 490 | |
 Glutamate Colorimetric Assay Kit |
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| K629-100 | Biovision | EUR 539 | |
 Fumarate Colorimetric Assay Kit |
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| K633-100 | Biovision | EUR 512 | |
 Heme Colorimetric Assay Kit |
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| K672-100 | Biovision | EUR 490 | |
Glutathione Colorimetric Assay Kit |
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| K261-100 | Biovision | EUR 512 | |
) Fructosamine Assay Kit (Colorimetric) |
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| K450-100 | Biovision | EUR 620 | |
) Fructose Assay Kit (Colorimetric) |
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| K439-100 | Biovision | EUR 501 | |
 Magnesium Colorimetric Assay Kit |
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| K385-100 | Biovision | EUR 479 | |
 Urea Colorimetric Assay Kit |
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| K375-100 | Biovision | EUR 539 | |
 Ammonia Colorimetric Assay Kit |
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| K370-100 | Biovision | EUR 539 | |
 Iron Colorimetric Assay Kit |
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| K390-100 | Biovision | EUR 550 | |
) Sodium Assay Kit (Colorimetric) |
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| K391-100 | Biovision | EUR 773 | |
 Calcium Colorimetric Assay Kit |
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| K380-250 | Biovision | EUR 430 | |
 Zinc Colorimetric Assay Kit |
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| K387-100 | Biovision | EUR 490 | |
 AMP Colorimetric Assay Kit |
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| K229-100 | Biovision | EUR 577 | |
 Phosphate Colorimetric Assay Kit |
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| K410-500 | Biovision | EUR 316 | |
) Lactate Assay Kit (Colorimetric) |
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| MET-5012 | Cell Biolabs | 100 assays | EUR 450 |
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Description: Our Lactate Assay Kit measures L-lactate in biological samples. Lactate is first oxidized by lactate oxidase, yielding pyruvate and hydrogen peroxide. The hydrogen peroxide released from this reaction is specifically detected by either a colorimetric or fluorometric probe in a 1:1 ratio. Lactate levels in unknown samples are determined based on a lactate standard curve. |
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) Glycogen Assay Kit (Colorimetric) |
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| MET-5022 | Cell Biolabs | 100 assays | EUR 479 |
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Description: Our Glycogen Assay Kit (Fluorometric) measures glycogen in serum, plasma, urine, lysates, and cell culture supernatants. First, glycogen is broken down into glucose monomers by amyloglucosidase. Glucose is then oxidized by glucose oxidase, producing D-gluconic acid and hydrogen peroxide. The hydrogen peroxide reacts specifically with the kit?s Fluorometric Probe and is detected at ex. 530-570 nm/em. 590-600 nm. Glycogen levels in unknown samples are determined based on the provided glycogen standard curve. |
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) Starch Assay Kit (Colorimetric) |
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| MET-5025 | Cell Biolabs | 100 assays | EUR 479 |
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Description: Our Starch Assay Kit (Colorimetric) measures starch in food samples. First, starch is broken down into glucose monomers by amyloglucosidase. Glucose is then oxidized by glucose oxidase, producing D-gluconic acid and hydrogen peroxide. The hydrogen peroxide reacts specifically with the kit?s Colorimetric Probe and is detected with a spectrophotometric plate reader at 540-570nm. Starch levels in unknown samples are determined based on the provided starch standard curve. |
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) Choline Assay Kit (Colorimetric) |
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| MET-5043 | Cell Biolabs | 96 assays | EUR 473 |
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Description: Cell Biolabs? Choline Assay Kits are simple assays that measure the amount of choline present in a variety of sample types in a convenient 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, acetylcholine standards and samples. Sample choline concentrations are determined by comparison with a known choline standard and measured on a colorimetric plate reader. |
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) Glutamate Assay Kit (Colorimetric) |
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| MET-5080 | Cell Biolabs | 200 assays | EUR 490 |
) Acetylcholine Assay Kit (Colorimetric) |
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| STA-603 | Cell Biolabs | 96 assays | EUR 473 |
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Description: Cell Biolabs? Acetylcholine Assay Kits are a simple fluorometric or colorimetric assay that measures the amount of acetylcholine present in plasma or serum, tissue homogenates, or cell suspensionsin a 96-well microtiter plate format. Each kit provides sufficient reagents to perform up to 96 assays, including blanks, acetylcholine standards and samples. Sample acetylcholine concentrations are determined by comparison with a known acetylcholine standard. |
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) Alcohol Assay Kit (Colorimetric) |
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| STA-620 | Cell Biolabs | 100 assays | EUR 519 |
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Description: Cell Biolabs? Alcohol Assay Kits measure primary alcohols by an enzymatic, oxidation reaction, producing hydrogen peroxide which reacts with the kit?s probe. These assay kits come in either colorimetric or fluorometric. The colorimetric assay has a detection sensitivity limit of ~30 µM (0.0001 % w/v) and the fluorometric has a detection sensitivity limit of ~15 µM (0.00007 % w/v). They can detect various primary alcohols and are not ethanol specific. |
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) Glucose Assay Kit (Colorimetric) |
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| STA-680 | Cell Biolabs | 500 assays | EUR 537 |
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Description: The Glucose Assay Kit (Colorimetric) measures total glucose present in food or biological samples. Glucose Oxidase first oxidizes glucose, generating hydrogen peroxide that is detected by a colorimetric probe. |
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Calcium Colorimetric Assay Kit |
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| K2067-250 | ApexBio | 250 assays | EUR 432 |
Magnesium Colorimetric Assay Kit |
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| K2068-100 | ApexBio | 100 assays | EUR 502 |
Iron Colorimetric Assay Kit |
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| K2069-100 | ApexBio | 100 assays | EUR 544 |
Phosphate Colorimetric Assay Kit |
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| k2074-500 | ApexBio | 500 assays | EUR 293 |
Hydroxyproline Colorimetric Assay Kit |
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| K2083-100 | ApexBio | 100 assays | EUR 599 |
Glutamine Colorimetric Assay Kit |
|||
| K2084-100 | ApexBio | 100 assays | EUR 641 |
Glutathione Colorimetric Assay Kit |
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| K2106-100 | ApexBio | 100 assays | EUR 529 |
Glutamate Colorimetric Assay Kit |
|||
| K2133-100 | ApexBio | 100 assays | EUR 544 |
Malate Colorimetric Assay Kit |
|||
| K2139-100 | ApexBio | 100 assays | EUR 502 |
Hexokinase Colorimetric Assay Kit |
|||
| K2179-100 | ApexBio | 100 assays | EUR 627 |
Acetoacetate Colorimetric Assay Kit |
|||
| K2204-100 | ApexBio | 100 assays | EUR 795 |
Acetate Colorimetric Assay Kit |
|||
| K2209-100 | ApexBio | 100 assays | EUR 529 |
Heme Colorimetric Assay Kit |
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| K2215-100 | ApexBio | 100 assays | EUR 502 |
 soluble collagen assay kit 2x 96-assays |
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| QZBcol1-2 | Quickzyme | 2 plates 192 assays | EUR 614 |
 soluble collagen assay kit 5x 96-assays |
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| QZBcol1-5 | Quickzyme | 5 plates 480 assays | EUR 1196 |
 sensitive tissue collagen assay kit 96-assays |
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| QZBtiscol1 | Quickzyme | 1 plate 96 assays | EUR 411 |
 total collagen assay kit 2x 96-assays |
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| QZBtotcol2 | Quickzyme | 2 plates 192 assays | EUR 614 |
 total protein assay kit 2x 96-assays |
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| QZBtotprot2 | Quickzyme | 2 plates 192 assays | EUR 527 |
) MTT Cell Viability Assay Kit (1000 assays) |
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| 30006 | Biotium | 1KIT | EUR 204 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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) Resazurin Cell Viability Assay Kit (2500 assays) |
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| 30025-1 | Biotium | 1KIT | EUR 181 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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 Canine nitric oxide synthase ELISA Kit |
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| ELA-E0628d | Lifescience Market | 96 Tests | EUR 928 |
 Rabbit nitric oxide synthase ELISA Kit |
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| ELA-E0628Rb | Lifescience Market | 96 Tests | EUR 928 |
 Human Total Nitric Oxide ELISA kit |
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| E01N0041-192T | BlueGene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Human Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Total Nitric Oxide ELISA kit |
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| E01N0041-48 | BlueGene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Human Total Nitric Oxide in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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This was a potential single heart investigation carried out in 120 sufferers with ACS who have been handled with prasugrel or ticagrelor as per normal of care. Patients who underwent percutaneous coronary interventions (PCI) have been handled with a loading dose of prasugrel (60 mg) or ticagrelor (180 mg), and sufferers who have been on upkeep remedy have been taking prasugrel (10 mg qd or 5 mg qd) or ticagrelor (90 mg bid). Platelet operate testing was carried out utilizing the VerifyNow™ PRUTest™. The total vary of PRUTest values was decrease than that noticed in research of sufferers handled with clopidogrel. The use of a upkeep dose routine had a wider vary of PRUTest values in comparison with using a loading dose for each prasugrel (1-179 vs. 2-128) and ticagrelor (1-196 vs. 1-177).
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